A latex fixation test using British latex and bovine gamma globulin

A Broader Concept of Caplan's Syndrome Related to Rheumatoid Factors

“…A latex fixation test using bovine gamma globulin as the reactant (Payne, 1961) was performed on 241 sera. Agglutination at an initial serum dilution of 1 in 80 or greater was considered positive.…”

A simple latex slide test for rheumatoid arthritis using the gamma globulin in the patient's serum

“…The ability of normal sera to inhibit agglutination was lost at a dilution greater than 1 in 10. Payne (1961) found that when bovine y globulin was added to a latex suspension the particles agglutinated at certain concentrations of y globulin but not at others. It may be that a similar phenomenon takes place when serum is diluted.…”

Evaluation of a New Preserved Latex Antigen for the Sero-Diagnosis of Rheumatoid Arthritis

“…Burby and Behr (1958) found that Dow 0 81 , particles sensitized with bovine globulin were as satisfactory for this test as were those sensitized with human gamma globulin, and the same authors (1959) also reported that a new polystyrene latex particle manufactured in Great Britain gave results equivalent to those obtained with the Dow particles, if used in a different dilution. Payne (1961) described a new procedure using the British latex particles sensitized with bovine gamma globulin in an alkaline buffer. He claimed that his procedure showed good agreement with the sensitized sheep cell agglutination test (Jacobson, Kammerer, Wolf, Epstein, and Heller, 1956) and F-TI L.P. tests, and was technically simpler.…”

“…This antigent is prepared from polystyrene latex particles, similar to those used by Payne (1961), sensitized with bovine gamma globulin. The advantages claimed for this antigen are that it is economical, stable, and simple to use, and an evaluation was therefore conducted to compare this material with other widely used latex procedures in the sero-diagnosis of rheumatoid factor.…”