A lateral flow immunoassay method for the rapid detection of acetochlor and alachlor in vegetable oil by sensitivity enhancement by using dimethyl-β-cyclodextrin

Li, Zepeng; Wu, Yuxiang; Li, Zijing; Yu, Binger; Mao, Xinyi; Shi, Guoqing

doi:10.1039/d3ay01379k

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…Nevertheless, the potential differences among stereoisomers have hardly ever been investigated, as well as antibodies produced by them. Moreover, most immunoassays were reported on the detection of the racemate, such as alachlor, , acelachlor, , and metolachlor. , Neither did these studies focus on the detection of chiral compounds nor did they involve the production of highly stereospecific antibodies. Therefore, it is necessary to study the recognition between the antigen and enantioselective antibody from the perspective of enantiomers.…”

Section: Introductionmentioning

confidence: 99%

Central Chirality and Axial Chirality Recognition of the Enantioselective Antibodies to Herbicide Metolachlor

Shen,

Zhang,

et al. 2024

J. Agric. Food Chem.

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Enantioselective antibodies have emerged as efficient tools in the field of chiral chemical detection and separation. However, it is complicated to obtain a highly stereoselective antibody due to the unclear recognition mechanism. In this study, the hapten of metolachlor was synthesized and enantio-separated. The absolute configuration of the four haptens obtained was identified by the computed and experimental electronic circular dichroism comparison. Five polyclonal antibodies against the Rac-metolachlor and its enantiomers were generated by immunization. The cross-activity of all the 5 antibodies with 44 structural analogues, including metolachlor enantiomers, was tested. It demonstrated that antibodies have higher specificity to recognize central chirality than axial chirality. Especially, αRR-MET-Ab exhibited excellent specificity and stereoselectivity. Accordingly, 3D-QSAR models were constructed and revealed that paired stereoisomers exhibited opposite interactions with the antibodies. It is the first time that the antibodies against four stereoisomers were prepared and analyzed, which will be conducive to the rational design of the stereoselective antibodies.

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Section: Introductionmentioning

confidence: 99%

Central Chirality and Axial Chirality Recognition of the Enantioselective Antibodies to Herbicide Metolachlor

Shen,

Zhang,

et al. 2024

J. Agric. Food Chem.

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Pesticides in the environment: Degradation routes, pesticide transformation products and ecotoxicological considerations

Mitra,

Saran,

Srivastava

et al. 2024

Science of The Total Environment

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A lateral flow immunoassay method for the rapid detection of acetochlor and alachlor in vegetable oil by sensitivity enhancement by using dimethyl-β-cyclodextrin

Abstract: Acetochlor is an endocrine disruptor. The acetochlor residue is strongly lipophilic and can be enriched into products during the manufacturing process. In this study, we found that dimethyl-β-cyclodextrin (DM- β-...

Cited by 2 publications

References 24 publications

Central Chirality and Axial Chirality Recognition of the Enantioselective Antibodies to Herbicide Metolachlor

Central Chirality and Axial Chirality Recognition of the Enantioselective Antibodies to Herbicide Metolachlor

Pesticides in the environment: Degradation routes, pesticide transformation products and ecotoxicological considerations

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