A lateral electrophoretic flow diagnostic assay

Lin, Robert; Skandarajah, Arunan; Gerver, Rachel E.; Neira, Hector D.; Fletcher, Daniel A.; Herr, Amy E.

doi:10.1039/c4lc01370k

Cited by 29 publications

(17 citation statements)

References 57 publications

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“…Capture probes Adsorption - [46] Capture probes Hydrogel Stimulus (temperature) [82] Capture probes Nanocarriers - [51] Detection probes Liquid Pressure [69] Detection probes Liquid Electrophoresis [60] Detection Probes Adsorption Dissolution [77] Detection probes Nanocarriers - [57] Enzymes DNA polymerase Liquid Pressure [18] DNA polymerase lyophilization Dissolution [35] Horseradish peroxidase Adsorption Dissolution [28] Invertase Nanocarriers Stimulus (targets) [87] Nucleic Particles with probes Liquid Centrifugation [20] Particles with probes Adsorption Dissolution [72] Particles with probes Lyophilization Dissolution [31] Other substances NaOH Adsorption Dissolution [86] Glucose Nanocarriers Stimulus (targets) [54] MgOAc Adsorption Dissolution [66] www.advmattechnol.de ( Figure 4D). They use sodium perborate to replace H 2 O 2 as the oxidant of CL reaction and they lyophilize the CL substrate on the glass fiber for the assembly of the CLFA.…”

Section: Antibodiesmentioning

confidence: 99%

“…By immobilizing the probes with an encoding pattern, this microfluidic chip can be used for multiplex detection of microRNAs, which is quite meaningful for microRNA diagnostics. A lateral electrophoretic flow assay was reported for fast confirmatory diagnosis [60] ( Figure 6B). The microchannel is filled with photoactive polyacrylamide hydrogel.…”

Section: Hydrogel For Reagents Storagementioning

confidence: 99%

Section: Manipulation Of Liquid Reagentsmentioning

confidence: 99%

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Advances in Reagents Storage and Release in Self‐Contained Point‐of‐Care Devices

Deng

Jiang

2019

Adv Materials Technologies

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Microfluidics has emerged as a useful material for point‐of‐care testing. However, conventional microfluidic devices rely on multiple steps to complete the detection process, which may not be acceptable for untrained users. The emerging “self‐contained” point‐of‐care devices, with all necessary reagents prestored in, address this limitation. This paper summarizes the recent advances of self‐contained point‐of‐care devices, with emphasis on the approaches for reagents integration and manipulation. Due to the intrinsic similarity and relevance of these approaches in pharmaceutical applications, several examples about drug delivery in vivo are presented. Different approaches are compared and future trends for the development of self‐contained point‐of‐care devices are outlined.

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Section: Antibodiesmentioning

confidence: 99%

Section: Hydrogel For Reagents Storagementioning

confidence: 99%

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Advances in Reagents Storage and Release in Self‐Contained Point‐of‐Care Devices

Deng

Jiang

2019

Adv Materials Technologies

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“…A wide variety of synthetic nanomaterials have been explored as alternatives to colloidal gold, including fluorescent 20 or luminescent 21 nanoparticles, 22 quantum dots 23 , iron oxides, 24 and phosphors. 25, 26 Similarly, alternate methods to modulate the flow of reporters through the strip have been proposed, including electrophoresis, 27 isotachophoresis, 28 and flow delay. 29 Finally, proposed ultrasensitive readouts include volumetric magnetization, 30, 31 near-infrared detection, 32 surface-enhanced Raman spectroscopy, 33 fluorescence, 34, 35 chemiluminescence, 9, 36 phosphorescence 37 , and thermal contrast.…”

Section: Introductionmentioning

confidence: 99%

Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay

Kim

Poling-Skutvik

Trabuco

et al. 2017

Analyst

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Using microscopy and image analysis, we characterize binding of filamentous viral nanoparticles to a fibrous affinity matrix as models for reporter capture in a lateral flow assay (LFA). M13 bacteriophage (M13) displaying an in vivo-biotinylated peptide (AviTag) genetically fused to the M13 tail protein p3 are functionalized with fluorescent labels. We functionalize glass fiber LFA membranes with antibodies to M13, which primarily capture M13 on the major p8 coat proteins, or with avidin, which captures M13 at the biotin-functionalized tail, and compare orientational modes of reporter capture for the side- versus tip-binding recognition interactions. The number of captured M13 is greater for side-binding than for tip-binding, as expected from the number of recognition groups. Whereas two-thirds of side-bound M13 captured by an anti-M13 antibody bind immediately after colliding with the membrane, tip-bound M13 prominently exhibit three additional orientational modes that require M13 to reorient to enable binding. These results are consistent with the idea that the elongated M13 shape couples with the complex flow field in an open and disordered fibrous LFA membrane to enhance capture.

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“…For some cases, microfluidic-based immunoassays encounter long processing time for blocking treatments in the presence of complex device patterns. A lateral hydrogel-deprived chip for a barcode sandwich immunoassay presented a hepatitis C viral (HCV) in 1 h using low power electrophoresis [64]. In an effort to manage global health or understand causes of diseases thoroughly, the influences of environmental contaminants or food hazards must not be neglected.…”

Section: Immunoassaymentioning

confidence: 99%