A laser fluorimeter for direct cardiac metabolism investigation

Renault, G.; Raynal, E.; Sinet, Martine; Berthier, J. P.; Godard, Bruno; Cornillault, J.

doi:10.1016/0030-3992(82)90110-4

Cited by 41 publications

(17 citation statements)

References 16 publications

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“…2. Renault and collaborators used a light guide fluorometer for monitoring heart in vivo (206,207). Rex and collaborators used another type of light guide fluorometer for the brain and other systems (202,208).…”

Section: Monitoring Devices and Technological Aspectsmentioning

confidence: 99%

“…The laser-based fluorimeter developed by Renault (207) was used to monitor NADH redox state in the heart muscle during pharmacological treatments (207), as well as in skeletal muscle (91). Attempts to apply NADH fluorometry in clinical practice (reported in a dozen short publications) did not lead to the development of a proper medical device applicable on a daily basis.…”

Section: Monitoring Human Body Organsmentioning

confidence: 99%

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Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies

Mayevsky¹,

Rogatsky²

2007

American Journal of Physiology-Cell Physiology

327

290

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Mayevsky A, Rogatsky GG. Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies. Am J Physiol Cell Physiol 292: C615-C640, 2007. First published August 30, 2006; doi:10.1152/ajpcell.00249.2006.-Normal mitochondrial function is a critical factor in maintaining cellular homeostasis in various organs of the body. Due to the involvement of mitochondrial dysfunction in many pathological states, the real-time in vivo monitoring of the mitochondrial metabolic state is crucially important. This type of monitoring in animal models as well as in patients provides real-time data that can help interpret experimental results or optimize patient treatment. The goals of the present review are the following: 1) to provide an historical overview of NADH fluorescence monitoring and its physiological significance; 2) to present the solid scientific ground underlying NADH fluorescence measurements based on published materials; 3) to provide the reader with basic information on the methodologies used in the past and the current state of the art fluorometers; and 4) to clarify the various factors affecting monitored signals, including artifacts. The large numbers of publications by different groups testify to the valuable information gathered in various experimental conditions. The monitoring of NADH levels in the tissue provides the most important information on the metabolic state of the mitochondria in terms of energy production and intracellular oxygen levels. Although NADH signals are not calibrated in absolute units, their trend monitoring is important for the interpretation of physiological or pathological situations. To understand tissue function better, the multiparametric approach has been developed where NADH serves as the key parameter. The development of new light sources in UV and visible spectra has led to the development of small compact units applicable in clinical conditions for better diagnosis of patients.real-time tissue viability; tissue spectroscopy; patient monitoring UNDERSTANDING THE MITOCHONDRIAL function has been a challenge for many investigators, including cytologists, biochemists, and physiologists, since its discovery more than 120 years ago. In addition to many books regarding the mitochondria, Ernster and Schatz (79) reviewed the history of mitochondrial structure and function studies. In the past two decades, several studies have reported mitochondrial involvement in pathological processes such as stroke (225) or cytoprotection (77). Most of the information on the mitochondrial function has been accumulated from in vitro studies. A relatively small portion of published papers dealt with the monitoring of mitochondrial function in vivo and in real time. Presently, examination of the involvement of the mitochondrial function in many pathological states, such as sepsis, requires monitoring of patients treated in intensive care units. Unfortunately, real-time monitoring of the mitochondrial function in patients has rarely been performed. The current study pres...

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Section: Monitoring Devices and Technological Aspectsmentioning

confidence: 99%

Section: Monitoring Human Body Organsmentioning

confidence: 99%

Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies

Mayevsky¹,

Rogatsky²

2007

American Journal of Physiology-Cell Physiology

327

290

View full text Add to dashboard Cite

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“…This change can be detected optically because NADH is a fluorescent molecule (excitation wavelength 340 nm, emission wavelength 480 nm), but NAD is not. NADH fluorescence has proven to be a particularly sensitive and specific method of monitoring metabolism [1][2][3][4][5][6][7][8][9][10][11][12]. Using NADH fluorescence, we investigated the effects of an enriched oxygen reperfusion solution on an isolated perfused rat heart model after a period of global ischemia.…”

Section: Introductionmentioning

confidence: 99%

Monitoring myocardial reperfusion injury with NADH fluorometry

et al. 1992

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Using NADH fluorometry to monitor myocardial metabolism, the mechanism of reperfusion injury was investigated after the delivery of an experimental reperfusate. Using an isolated working heart preparation, rat hearts underwent 15 min of global ischemia at 37 degrees C. Following the ischemic insult, an oxygenated enriched reperfusion solution was given for 5 min. The hearts were then returned to a working state and aortic flow recorded to evaluate recovery. NADH levels were monitored throughout the experiment with a fluorometer and glycogen, AMP, ADP, and ATP were measured biochemically pre- and postischemia, after reperfusion and after recovery. In this study, reperfusion injury was best abated by an enriched reperfusate. Our results indicate the mechanism for this amelioration is not high-energy phosphate replenishment. Rather, as indicated by NADH fluorescence, the hearts attain an intermediate level of metabolism that permits glycogen to be restored and functional recovery to be improved.

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“…The in situ laser fluorimeter (Cilas-Alcatel) includes the following: a nitrogen laser which provides the excitation light for NADH (A, = 337 nm; pulse duration = 3 ns, repetition rate = 50 Hz); a dye laser, pumped by the nitrogen laser, which provides the reference light (A2 = 586 nm); an accurate optical system of lens, filters, beam splitters, and mirrors [8]; a single optical fiber, with especially designed endfaces [9] for conduction of optical signals between the optical system and the biological tissue (Medisil 400 from "fibres optiques industries"; length 5 m; core diameter = 0.4 mm); an analog system system for data processing: fluorescence (F) at 480 nm, backward-scattering (I) at 586 nm; the final analog data F and I were displayed on-line on a chart recorder (Gould 4402) and simultaneously entered in an HP 85 (Hewlett-Packard) microcomputer after analog to digital conversion. The digital processing consisted of storage on digital tapes, computation of compensated fluorescence F, according to a mathematical relationship which we had previously demonstrated [ 101 : …”

Section: Methodsmentioning

confidence: 99%

“…Therefore, following the initial works of Pr. Chance and his group, in vivo fluorimetry of NADH was developed for on-line noninvasive studies of living tissue metabolism in various experimental models [ 1-71. We have developed in situ laser fluorimeter [8] to extend this method to the study of in situ normally blood-perfused organs in their usual environment. The final purpose was to achieve a new kind of human clinical investigation.…”

Section: Introductionmentioning

confidence: 99%

In situ monitoring of myocardial metabolism by laser fluorimetry: Relevance of a test of local ischemia

et al. 1985

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We have developed a hypoxia test by local ischemia, to be performed with a special probe adapted to an in situ NADH laser fluorimeter. Local ischemia is produced by pressurization on the organ surface in an area of approximately 0.2 mm2. In order to assess the method on open-chest rat hearts (ten), we used the following protocol: local ischemia tests (three), global anoxia (100% N2 ventilation), superimposition of local ischemia to global anoxia, and local ischemia tests during the period just prior to death and immediately thereafter. Three different responses were observed: large amplitude of compensated fluorescence (Fo) increase, medium amplitude of Fo increase, and no Fo increase. These responses were related to the metabolic state prior to the test (States 3, 4, and 5 of Chance's nomenclature). We have thus demonstrated the possibility of very rapidly determining the in situ NADH degree of reduction, without a destructive assay. Such a parameter may be of great relevance in heart surgery, as it might allow detection of potentially harmful situations, thereby enabling early and appropriate treatment.

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A laser fluorimeter for direct cardiac metabolism investigation

Cited by 41 publications

References 16 publications

Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies

Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies

Monitoring myocardial reperfusion injury with NADH fluorometry

In situ monitoring of myocardial metabolism by laser fluorimetry: Relevance of a test of local ischemia

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