A large-scale purification of paclitaxel from cell cultures of Taxus chinensis

Pyo, Sang‐Hyun; Park, Heung-Bok; Song, Bong-Kyu; Han, Byung-Hee; Kim, Jin-Hyun

doi:10.1016/j.procbio.2003.09.028

Cited by 75 publications

(22 citation statements)

References 16 publications

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“…According to a previous study [9], the optimum ratio of solvent to biomass and extraction temperature in the CSE process was confirmed to be 1:1 (v/w) and room temperature, respectively. First, the effect of extraction time (10, 20, 30 min) and extraction number (1, 2, 3 times) were investigated in the extraction using an ionic liquid under the optimum conditions.…”

Section: Extraction Using An Ionic Liquid As Co-solventmentioning

confidence: 78%

“…The biomass from plant cell cultures was mixed with methanol and stirred at room temperature for 30 min [9]. The mixture was filtered under vacuum in a Buchner funnel through filter paper (150 mm, Whatman), where the methanol was preferably added to biomass at a ratio of 1:1 (mL/g, v/w).…”

Section: Conventional Solvent Extraction (Cse)mentioning

confidence: 99%

“…Most of the paclitaxel produced by plant cell culture is contained in plant cells and debris [6] and it is important to efficiently extract paclitaxel included in cells to increase recovery. In the existing extraction methods, paclitaxel has been primarily recovered from biomass, which is plant cells, using organic solvents [7][8][9]. However, the existing solvent extraction (conventional solvent extraction, CSE) method has drawbacks such as requiring a long extraction time and a large amount of organic solvents, as well as a low extraction efficiency.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of extraction efficiency of paclitaxel from biomass using ionic liquid-methanol co-solvents under acidic conditions

Kim

2015

Process Biochemistry

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Section: Extraction Using An Ionic Liquid As Co-solventmentioning

confidence: 78%

Section: Conventional Solvent Extraction (Cse)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhancement of extraction efficiency of paclitaxel from biomass using ionic liquid-methanol co-solvents under acidic conditions

Kim

2015

Process Biochemistry

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“…Traditional processes of isolation and purification of paclitaxel from yew tree and plant cell cultures involve generally multiple steps of liquid-liquid partitioning, enrichment, purification by liquid chromatography, such as; low-pressure liquid chromatography [21][22][23][24], and preparative high-performance liquid chromatography (HPLC) [25,26], industrial preparative liquid chromatography [27], counter current chromatography [28][29][30], and simulated moving-bed chromatography [31]. In addition, these separation procedures adopted loose decent quantities of paclitaxel and also are bothered by interfering substances such as cephalomannine and 10-deacetyl-7-epi-paclitaxel, which are difficult to separate from paclitaxel [22,29,30].…”

Section: Introductionmentioning

confidence: 99%

Efficient purification of paclitaxel from yews using high-performance displacement chromatography technique

Watchueng

Kamnaing

Gao

et al. 2011

Journal of Chromatography A

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Efficient purification of paclitxel from yews using high-performance displacement chromatography technique Watchueng, J.; Kamnaing, P.; Gao, JM.; Kiyota, T.; Yeboah, F.; Konishi, Y.Journal of Chromatography A, 1218Chromatography A, (2011Chromatography A, ) 2929Chromatography A, -2935 Paclitaxel was purified using high-performance displacement chromatography (HPDC) technique, but not by the mechanism of HPDC. On small scale, paclitaxel was extracted with methanol from dry needles of Taxus canadensis and was enriched by extracting with chloroform after removing water-soluble hydrophilic components and hexane-soluble hydrophobic components. Then, 93-99% purity of paclitaxel was obtained using the HPDC technique. On large scale, taxanes were enriched by solvent partitioning between acetic acid/MeOH/H 2 O and hexane and extracted with CH 2 Cl 2 . Taxanes except paclitaxel were further removed by extracting with methanol-water-trifluoroacetic acid (1.0:98.9:0.1, v/v/v). Applying HPDC technique to water-insoluble substances is problematic as this method requires a highly aqueous solvent system. In order to overcome this incompatibility, a system was set up where paclitaxel, although in low concentration, was extracted by methanol-water-trifluoroacetic acid (10.0:89.9:0.1, v/v/v). Recycling the extracting solvent to ensure minimal volume, the extracted paclitaxel was adsorbed on a C 18 trap column. A C 18 column of 4.6 mm internal diameter was then connected to the trap column. The HPDC technique was thus carried out using an isocratic acetonitrile-water-trifluoroacetic acid (30.0:69.9:0.1, v/v/v) mobile phase consisting of a displacer cetylpyridinium trifluoroacetate (3 mg/mL). Paclitaxel was co-eluted with the displacer and spontaneously crystallized. The crystal (114 mg) showed 99.4% purity and only 10% of paclitaxel in the starting crude extract was lost during the enrichment/purification processes. This large scale purification method was successfully applied to purify paclitaxel from Chinese yew in small scale, suggesting general applicability of the method. This is the first report of purifying a water-insoluble natural product using HPDC technique.Crown

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“…Since the yield of purified paclitaxel from T. brevifolia is very low (about 0.04% of the bark dry weight), and bark-stripping leads to the destruction of scarce plant material [4], many attempts have been made to develop new methods for the reliable production of paclitaxel from renewable resources including semi-synthesis, total synthesis, and plant cell culture strategies [5][6][7][8]. In the search for other bioactive compounds, many other taxane derivatives have been isolated or synthesized chemically for structure-activity relationship studies [4,[9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of paclitaxel rearrangement involving opening of the oxetane ring and migration of acetyl and benzoyl groups

Pyo¹,

Jinsuk²,

Choi³

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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A large-scale purification of paclitaxel from cell cultures of Taxus chinensis

Cited by 75 publications

References 16 publications

Enhancement of extraction efficiency of paclitaxel from biomass using ionic liquid-methanol co-solvents under acidic conditions

Enhancement of extraction efficiency of paclitaxel from biomass using ionic liquid-methanol co-solvents under acidic conditions

Efficient purification of paclitaxel from yews using high-performance displacement chromatography technique

Evaluation of paclitaxel rearrangement involving opening of the oxetane ring and migration of acetyl and benzoyl groups

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