A LAMP-Based Toolbox Developed for Detecting the Major Pathogens Affecting the Production and Quality of the Chinese Medicinal Crop Aconitum carmichaelii

Abstract

Aconitum carmichaelii Debeaux is a traditional Chinese medicinal herb that has been utilized for approximately two thousand years. However, as cultivation has increased, there have been more reports of A. carmichaelii infections caused by four major pathogenic fungal species, Fusarium oxysporum, F. solani, Mucor circinelloides, and Sclerotium rolfsii, resulting in increased disease incidences and limited production and quality. To detect these infections, we developed a LAMP-based toolbox in this study. The cytochrome c oxidase subunit 1 (cox1) gene, translation elongation factor-1α (EF-1α), internal transcribed spacer (ITS) regions of rDNA, and alcohol dehydrogenase 1 (ADH1) gene, respectively, were used to design species-specific LAMP primer sets for F. oxysporum, F. solani, S. rolfsii, and M. circinelloides. The results showed that the LAMP-based toolbox was effective at detecting pathogens in soil and plant materials. We also used this toolbox to investigate pathogen infection in the main planting regions of A. carmichaelii. Before harvesting, F. oxysporum, M. circinelloides, and S. rolfsii, were commonly found in the planting fields and in infected A. carmichaelii plants. Therefore, the toolbox we developed will be useful for tracking these infections, as well as for disease control in A. carmichaelii.

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

“…Due to the mineral oil overlay, LAMP-amplified products were not recovered for further testing, and electrophoresis was not performed. Furthermore, many researchers have reported that colour change results were consistent with electrophoresis results [42,43]. Therefore, LAMP is an alternative nucleotide amplification method that is rapid, simple, highly sensitive, and suitable for analysing pathogens in the field.…”

Virome Analysis of Aconitum carmichaelii Reveals Infection by Eleven Viruses, including Two Potentially New Species

“…Among these regions, the longest period of A. carmichaelii cultivation dates back more than a thousand years in Jiangyou City, Sichuan Province. Since 2015, A. carmichaelii cultivation has expanded to approximately 3000 hectares in Shaanxi and Sichuan Provinces, establishing it as a significant local industry [5]. A. carmichaelii plants are cultivated through seed and by vegetative propagation from lateral roots.…”