A laccase–glucose oxidase biofuel cell prototype operating in a physiological buffer

Barrière, Frédéric; Kavanagh, Paul; Leech, Dónal

doi:10.1016/j.electacta.2006.03.050

Cited by 202 publications

(134 citation statements)

References 36 publications

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“…Glucose/air biofuel cells have been designed using GOx at the anode and laccase at the cathode to reduce air. Redox polymers containing osmium centers have been used as mediators to aid electron transfer in the absence of CNTs [147]. However, addition of CNT to the electrodes along with enzyme incorporation by simple mechanical compaction significantly increased the performance of the glucose/air biofuel cells [146].…”

Section: Energymentioning

confidence: 99%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

188

111

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a b s t r a c tSince the discovery of low-dimensional carbon allotropes, there is increasing interest in using carbon nanomaterials for biomedical applications. Carbon nanomaterials have been utilized in the biomedical field for bioimaging, chemical sensing, targeting, delivery, therapeutics, catalysis, and energy harvesting. Each application requires tailored surface functionalization in order to take advantage of a desired property of the nanoparticles. Herein, we review the surface immobilization of bio-molecules, including proteins, peptides, and enzymes, and present the recent advances in synthesis and applications of these conjugates. The carbon scaffold and the biological moiety form a complex interface which presents a challenge for achieving efficient and robust binding while preserving biological activity. Moreover, some applications require the utilization of the protein-nanocarbon system in a complex environment that may hinder its performance or activity. We analyze different strategies to overcome these challenges when using carbon nanomaterials as protein carriers, explore various immobilization techniques along with characterization methods, and present recent demonstrations of employing these systems for biomedical applications. Finally, we consider the challenges and future directions of this field.

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Section: Energymentioning

confidence: 99%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

188

111

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“…Besides fundamental understanding of how O 2 is bioelectrocatalytically reduced to H 2 O in nature avoiding intermediate formation of reactive oxygen species, applications in biosensors and membrane-less biofuel cells are of increasing importance [10][11][12][13][14][15][16]. Crucial for a membrane-less biofuel cell is that no cross reactivity between the cathode and anode reactions and moreover no reactions between the substrates used as fuels for the anode and cathode side occur.…”

mentioning

confidence: 99%

Design of a bioelectrocatalytic electrode interface for oxygen reduction in biofuel cells based on a specifically adapted Os-complex containing redox polymer with entrapped Trametes hirsuta laccase

Ackermann

Guschin

Eckhard

et al. 2010

Electrochemistry Communications

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The design of the coordination shell of an Os-complex and its integration within an electrodeposition polymer enables fast electron transfer between an electrode and a polymer entrapped high-potential laccase from the basidiomycete Trametes hirsuta. The redox potential of the Os 3+/2+ -centre tethered to the polymer backbone (+ 720 mV vs. NHE) is perfectly matching the potential of the enzyme (+ 780 mV vs. NHE at pH 6.5). The laccase and the Os-complex modified anodic electrodeposition polymer were simultaneously precipitated on the surface of a glassy carbon electrode by means of a pH-shift to 2.5. The modified electrode was investigated with respect to biocatalytic O 2 reduction to H 2 O. The proposed modified electrode has potential applications as biofuel cell cathode.

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“…19,20 Due to the DET process being correlated to enzyme proximity and orientation for electron tunneling between the active site and the current collector, the biocatalytic nature of the enzyme is described as being the limiting process. 21 Laccase has previously been used in many biocathode studies 15,[22][23][24][25] and is one of the most commonly recognized multi-copper oxidoreductases 26,27 consisting of three catalytic copper ions classified spectroscopically as Type 1, Type 2, and Type 3 sites (T1, T2, and T3, respectively). 28 The reduction of O 2 by laccase 16,29 is initially catalyzed by the T1 site which orients the O 2 substrate near a wide, hydrophobic binding pocket rich in π * Electrochemical Society Student Member.…”

mentioning

confidence: 99%

Operational Stability Assays for Bioelectrodes for Biofuel Cells: Effect of Immobilization Matrix on Laccase Biocathode Stability

Shrier

Giroud

Rasmussen

2014

J. Electrochem. Soc.

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One of the main issues of enzymatic biofuel cells is the need to develop stable bioelectrodes. However, there is no standard method to study bioelectrode stability. In this study, laccase and anthracene modified multiwall carbon nanotubes (anth-MWCNTs) were used in conjunction with different immobilization polymers in order to increase the stability of the biocathodes. A series of stability assays were used to understand the operational stability of the biocathode in a biofuel cell environment. The immobilization matrices used in this study were tetrabutyl ammonium bromide modified Nafion (TBAB modified Nafion), octyl modified linear polyethylenimine (C 8 -LPEI), and vapor deposited tetramethyl orthosilicate (TMOS) gels. The decrease in activity of the galvanostatic and potentiostatic measurements over a 24 hour period of the TBAB modified Nafion were 4.0 ± 0.6% and 4.1 ± 0.4%; C 8 -LPEI were 0.7 ± 0.1% and 9.6 ± 0.5%; and TMOS were 4.1 ± 0.1% and 10 ± 2.0%, respectively. This data show that potentiostatic measurements provide a harsher environment for the enzyme and result in lower stability, as well as showing that the TBAB modified Nafion offers a more stabilizing immobilization strategy compared to the C 8 -LPEI or TMOS matrices. The increasing interest in producing electrical energy from renewable resources has caused the field of biofuel cells to flourish. Enzymatic biofuel cell systems are capable of producing energy from renewable fuels. Basically, enzymatic biofuel cells (EBFC) generate electricity from the oxidation of the fuel at the anode and the reduction of the oxidant at the cathode using biological catalysts (enzymes) instead of traditional metal catalysts. More detailed descriptions of such biofuel cells can be found in reviews that discuss the fundamental concepts of this technology.1,2 Applications of EBFCs range from energy sources for small electronic devices, 3 self-powered sensors 4 and more recently as implantable power sources. [5][6][7] To improve this technology, much research has been focused on engineering and modifying the cathode half of these biofuel cells. [8][9][10][11][12][13][14][15][16][17][18] One such engineered bioelectrode system, previously developed by our group, employs anthracene modified multi-walled carbon nanotubes (anth-MWCNTs) for docking of the oxidoreductase enzyme laccase to the current collector. This biocathode uses anthracene covalently modified to the ends of the multi-walled carbon nanotubes (MWCNTs) in order to favorably orient the laccase enzyme to the ends of the carbon nanotubes, since laccase has a hydrophobic binding site for anthracene and the aromatic structure allows for improved conductivity between the active site and the carbon surface. The steric orientation provides increased catalytic reduction of oxygen to water and favorable distances such that the laccase performs direct electron transfer (DET). 8In biofuel cells, DET occurs through the enzyme's ability to oxidize or reduce a substrate while transferring the necessary electrons to or fro...

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A laccase–glucose oxidase biofuel cell prototype operating in a physiological buffer

Cited by 202 publications

References 36 publications

Protein functionalized carbon nanomaterials for biomedical applications

Protein functionalized carbon nanomaterials for biomedical applications

Design of a bioelectrocatalytic electrode interface for oxygen reduction in biofuel cells based on a specifically adapted Os-complex containing redox polymer with entrapped Trametes hirsuta laccase

Operational Stability Assays for Bioelectrodes for Biofuel Cells: Effect of Immobilization Matrix on Laccase Biocathode Stability

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