A Laboratory Study on the Behavior of Thiobacillus ferrooxidans during Pyrite Bioleaching in Percolation Columns

Monroy, Marcos; Dziurla, Marie-Antoinette; Lam, Bach-Tuyet; Berthelin, J.; Marion, Philippe

doi:10.1007/978-94-017-0641-4_71

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Compared to previous quantitative indirect methods using counting of cells remaining in suspension (4,19) and to qualitative methods using a combined immunofluorescence-DNAfluorescence staining procedure (21), the immunoenzymatic ELIFA method performed on filtration membranes and presented in this paper allowed the direct quantification of T. fer- rooxidans cells attached to pyrite particles. This method, performed with microtiter plates, is sensitive (10 4 bacteria were detected per well) and accurate.…”

Section: Discussionmentioning

confidence: 99%

“…The bacteria used in this study are listed in Table 1. T. ferrooxidans strains and isolates were grown in 72 mM ferrous sulfate or 2% (wt/vol) pyrite-M 2 basal salts medium (with compounds at the following concentrations [in grams per liter]: (NH 4 ) 2 SO 4 , 1.0; KH 2 PO 4 , 0.4; MgSO 4 ⅐ 7H 2 O, 0.4), pH 1.8 (19). Various other bacteria were also used to determine the specificity of the antiserum raised against T. ferrooxidans DSM 583.…”

Section: Materials and Methods Bacterial Strains And Preparation Of Cmentioning

confidence: 99%

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Enzyme-Linked Immunofiltration Assay To Estimate Attachment of Thiobacilli to Pyrite

Dziurla

Achouak

Lam

et al. 1998

Appl Environ Microbiol

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An enzyme-linked immunofiltration assay (ELIFA) has been developed in order to estimate directly and specifically Thiobacillus ferrooxidans attachment on sulfide minerals. This method derives from the enzyme-linked immunosorbent assay but is performed on filtration membranes which allow the retention of mineral particles for a subsequent immunoenzymatic reaction in microtiter plates. The polyclonal antiserum used in this study was raised againstT. ferrooxidans DSM 583 and recognized cell surface antigens present on bacteria belonging to the genusThiobacillus. This antiserum and the ELIFA allowed the direct quantification of attached bacteria with high sensitivity (104 bacteria were detected per well of the microtiter plate). The mean value of bacterial attachment has been estimated to be about 105 bacteria mg−1 of pyrite at a particle size of 56 to 65 μm. The geometric coverage ratio of pyrite by T. ferrooxidans ranged from 0.25 to 2.25%. This suggests an attachment of T. ferrooxidans on the pyrite surface to well-defined limited sites with specific electrochemical or surface properties. ELIFA was shown to be compatible with the measurement of variable levels of adhesion. Therefore, this method may be used to establish adhesion isotherms of T. ferrooxidans on various sulfide minerals exhibiting different physicochemical properties in order to understand the mechanisms of bacterial interaction with mineral surfaces.

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Section: Discussionmentioning

confidence: 99%

Section: Materials and Methods Bacterial Strains And Preparation Of Cmentioning

confidence: 99%

Enzyme-Linked Immunofiltration Assay To Estimate Attachment of Thiobacilli to Pyrite

Dziurla

Achouak

Lam

et al. 1998

Appl Environ Microbiol

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Bioleaching model of a copper-sulfide ore bed in heap and dump configurations

Casas

Vargas

Martínez

et al. 1998

Metall Mater Trans B

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A two-dimensional (2-D) model for a heap or dump bioleaching of a copper ore containing mainly chalcocite and pyrite has been developed. The rate of the mineral sulfide dissolution was related to the rate of oxidation by bacteria attached onto the ore surface. The latter was calculated using the model of Michaelis-Menten, where both temperature and dissolved oxygen in the leach solution were taken into account by the kinetic equation. Oxygen transport through the ore bed was associated with natural air convection originating from the decrease in gas density inside the ore bed, which was attributable not only to heating, but also to humidification and decrease in the oxygen concentration. The model was used to estimate air-velocity fields and profiles of temperature and oxygen concentrations as well as mineral conversions during the bioleaching operation for ore beds with different pyrite contents, bacterial populations, widths, heights, and permeabilities. The model provides a useful tool for the design, improvement, and optimization of industrial operating conditions.

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SEM and EDS observations of carrollite bioleaching with a mixed culture of acidophilic bacteria

Nkulu

Gaydardzhiev

Mwema

et al. 2015

Minerals Engineering

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A Laboratory Study on the Behavior of Thiobacillus ferrooxidans during Pyrite Bioleaching in Percolation Columns

Cited by 3 publications

References 6 publications

Enzyme-Linked Immunofiltration Assay To Estimate Attachment of Thiobacilli to Pyrite

Enzyme-Linked Immunofiltration Assay To Estimate Attachment of Thiobacilli to Pyrite

Bioleaching model of a copper-sulfide ore bed in heap and dump configurations

SEM and EDS observations of carrollite bioleaching with a mixed culture of acidophilic bacteria

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