SEM and EDS observations of carrollite bioleaching with a mixed culture of acidophilic bacteria

In this work, ceramic pellets were used as packing material to establish a biotrickling filter (BTF) with acclimated sludge being inoculated on the surface of the packing to purify waste gas containing styrene. A method of toluene-styrene secondary acclimation was applied to achieve rapid formation of biological films. Results showed that the total time of start-up was 48 days and the removal efficiency (RE) of styrene reached up to 95%. The suitable empty bed residence time (EBRT) was obtained that is 57 s for higher RE of styrene with the inlet loading rates of 6.7-271.6 g/m 3 /h. The pH and moisture content showed small effect on styrene removal indicating that the operation of BTF was stable. Biomass accumulation was normal and its rising velocity under the condition of short EBRT was faster than that of long EBRT.

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“…Finally, the bacteria were plated platinum to 10-20 nm by ion sputtering and were observed using S3400 N SEM. [27] 3. Results and discussion…”

Section: Methodsmentioning

confidence: 99%

Toluene-styrene secondary acclimation improved the styrene removal ability of biotrickling filter

Sun

Yang

Wang

et al. 2017

Chemical Speciation & Bioavailability

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“…Following this, the deposits are washed to recover the metal-laden microbes for down-stream processing. As well as microbes, bacterially derived extracellular polymers can also be added to promote growth of microbial cultures [ 11 ], these substances form at the interface between the spent-ore deposits and the bacteria to stimulate biofilm formation and cell-to-cell communication, to encourage an increase in metal recovery [ 12 ].…”

Section: Biominingmentioning

confidence: 99%

Synthetic biology approaches towards the recycling of metals from the environment

Capeness

Horsfall

2020

Biochemical Society Transactions

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Metals are a finite resource and their demand for use within existing and new technologies means metal scarcity is increasingly a global challenge. Conversely, there are areas containing such high levels of metal pollution that they are hazardous to life, and there is loss of material at every stage of the lifecycle of metals and their products. While traditional resource extraction methods are becoming less cost effective, due to a lowering quality of ore, industrial practices have begun turning to newer technologies to tap into metal resources currently locked up in contaminated land or lost in the extraction and manufacturing processes. One such technology uses biology for the remediation of metals, simultaneously extracting resources, decontaminating land, and reducing waste. Using biology for the identification and recovery of metals is considered a much ‘greener’ alternative to that of chemical methods, and this approach is about to undergo a renaissance thanks to synthetic biology. Synthetic biology couples molecular genetics with traditional engineering principles, incorporating a modular and standardised practice into the assembly of genetic parts. This has allowed the use of non-model organisms in place of the normal laboratory strains, as well as the adaption of environmentally sourced genetic material to standardised parts and practices. While synthetic biology is revolutionising the genetic capability of standard model organisms, there has been limited incursion into current practices for the biological recovery of metals from environmental sources. This mini-review will focus on some of the areas that have potential roles to play in these processes.

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“…De acordo comNkulu et al, (2015) foi utilizado na preparação das amostras para a análise no MEV-EDS, glutaraldeído 25 % e foi feito o enxágue com o meio 9K para eliminar o Fe da solução das amostras. A camada condutora utilizado foi de espessura de 20 nm de Pt e com esse procedimento foi possível visualizar o formato dos microrganismos [10].…”

Section: Análise Das Micrografiasunclassified

“…As etapas do processo deve-se à caracterização da amostra com o intuito de determinar os elementos químicos presentes e a inoculação sucessiva com o incremento de resíduos tem como objetivo aumentar a tolerância às concentrações dos metais solubilizados durante o processo de biolixiviação. Outros fatores levados em consideração foram as análises de amostras biológicas com a utilização de um microscópio eletrônico de varredura acoplado a um equipamento de espectroscopia de energia dispersiva de raios-X (MEV-EDS).A preparação das amostras biológicas deve-se à condição natural hidratada, apresenta características específicas durante o seu processamento para os resultados na visualização no MEV-EDS [10], [11]. O objetivo do estudo procura uma alternativa ambiental positiva para a recuperação de metais e uma tentativa de minimizar os resíduos dispostos em aterros.…”

Section: Introductionunclassified

Biohidrometalurgia: Recuperação De Cobre Proveniente De Reee Através Da Lixiviação Bacteriana

Utimura¹,

Botelho²,

Tenório³

et al. 2019

ABM Proceedings

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Resumo As placas de circuito impresso (PCIs) dos resíduos eletroeletrônicos (REEEs) possuem diversos materiais na composição. A recuperação dos metais tem incentivado as pesquisas no seu processo de extração. Como alternativa de recuperação de metais das PCIs de forma sustentável optou-se pela biohidrometalurgia envolvendo a fase extração. O presente estudo avalia a biolixiviação do cobre na presença da bactéria Acidithiobacillusferrooxidans(AF) em PCIs de REEEs provenientes de impressoras obsoletas. As PCIs foram caracterizadas mediante as técnicas analíticas de espectrometria ICP-OES e de fluorescência de Raios X (FRX) para determinar os elementos químicos presentes na amostra inicial. A bactéria utilizadas na lixiviação bacteriana foicultivada em meio de cultura T&K. Os ensaios de biolixiviação foram realizados em incubadora orbital, com velocidade de rotação de 170 rpm, temperatura de 30 o C, volume de inoculo de 10% (v/v) e densidade de polpa de 30g/L. Foi possível determinar que a cepa AFapresentou uma extração de 100% de cobre em 8 dias e foi analisado o MEV-EDS da bactéria AF.

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SEM and EDS observations of carrollite bioleaching with a mixed culture of acidophilic bacteria

Cited by 22 publications

References 21 publications

Toluene-styrene secondary acclimation improved the styrene removal ability of biotrickling filter

Toluene-styrene secondary acclimation improved the styrene removal ability of biotrickling filter

Synthetic biology approaches towards the recycling of metals from the environment

Biohidrometalurgia: Recuperação De Cobre Proveniente De Reee Através Da Lixiviação Bacteriana

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