A labeling chamber for 13C enrichment of plant tissue for decomposition studies

Berg, Jorrit D.J. van den; Hendrix, Paul F.; Cheng, Weixin; Dillard, Ashley

doi:10.1016/0167-8809(91)90125-h

Cited by 22 publications

(20 citation statements)

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“…64-71% of plant N and 83-84% of plant C were in this residue+rhizodeposited fraction. These values are of the same magnitude as those documented for other species with a low harvest index (Berg et al 1991;Stewart and Metherell 1998), and highlight the importance of residue inputs for recycling efficiency and potential provision of N and C to a subsequent crop. Of this fraction, 40-48% of the N, and 30-33% of C was below-ground demonstrating the potential significance of below-ground fractions in a crop Here further studies are required to quantify turnover of N in the below-ground fractions, to determine effects of abiotic factors on rhizodeposition, and to quantify uptake of N by a subsequent non-leguminous crop, before the real benefit of chickpea in rotation can be realised.…”

Section: Potential Benefit Of Chickpea N and C In Rotationmentioning

confidence: 54%

The significance of below-ground fractions when considering N and C partitioning within chickpea (Cicer arietinum L.)

2009

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The aim of this controlled environment experiment was to quantify the distribution of leaffed-15 N and canopy fed-13 C within nodulating, nonnodulating or N fertilized non-nodulating Cicer arietinum L. and in their surrounding rhizosphere soil, excluding soil+root respiration. Nodulating chickpea partitioned 32% of its total N and 27% of its total recoverable C below-ground, of which only 50% of N and 36% of C were in the clean root fraction. Non-nodulating chickpea allocated equal recoverable C but slightly less N (28%) below-ground but lost less C from plant induced below-ground respiration. The importance of this below-ground partitioning for crop systems C and N balances is highlighted by their large (45% and 33%, for N and C, respectively) contribution to the total plant derived residue (recyclable) fraction. Recovered 15 N and 13 C were greater (P<0.05) in the outer-rhizosphere (459µg 15 N and 3.2 mg 13 C core −1 ) than in the inner-rhizosphere soil (detached from roots during freeze-drying; 18µg 15 N and 67µg 13 C core −1 ) in relation with the relative size of these compartments. This highlights the significance of the outerrhizosphere soil when estimating C and N budgets and quantifying rhizodeposition, and the benefit of a double ( 15 N, 13 C) isotope approach to determine this flow against large background soil C and N pools.

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Section: Potential Benefit Of Chickpea N and C In Rotationmentioning

confidence: 54%

The significance of below-ground fractions when considering N and C partitioning within chickpea (Cicer arietinum L.)

2009

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“…In chamber labelling, typically the δ 13 C‐CO 2 value is not monitored, but rather the CO 2 concentration is recorded and kept within certain boundary values, for example, with an infrared gas analyser (e.g. Berg et al). Day‐night and long‐term fluctuations in the ambient air are only detected when atmospheric samples are taken at discrete points in time and analysed with the mass spectrometer.…”

Section: Introductionmentioning

confidence: 99%

Laser spectroscopy steered ¹³C‐labelling of plant material in a walk‐in growth chamber

Slaets

Resch

Mayr

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Carbon‐13 (13C)‐labelled plant material forms the basis for experiments elucidating soil organic carbon dynamics and greenhouse gas emissions. Quantitative field‐scale tracing is only possible if plants are labelled homogeneously in large quantities. By using a laser spectrometer to automatically steer the isotopic ratio in the chamber, it is possible to obtain large amounts of homogeneously labelled plant material. Methods Ninety‐six maize plants were labelled for 25 days until tassel formation in a 15 m3 walk‐in growth chamber with a continuous air δ13C‐CO2 value of 400‰. A Los Gatos Research laser absorption spectrometer controlled the ambient δ13C‐CO2 value in the chamber through steering of the mass flow controllers with 13C‐enriched and natural abundance CO2 gas. Results Laser absorption spectroscopy steering kept the δ13C value of chamber air between 368 and 426‰. The resulting 1 kg dry matter of 13C‐labelled shoots showed an average δ13C value of 384‰ and accuracy of 8‰ (half width of the 95% confidence interval). Only the oldest leaves showed larger heterogeneity. The growth chamber eliminated variability between plants. The δ13C value of the stabile material did not differ significantly from that of bulk material. Conclusions Laser spectroscopy controlled 13C labelling of plants in a walk‐in growth chamber successfully kept the isotopic ratio of the CO2 in the chamber air constant. Therefore, large quantities of material were labelled homogeneously at the inter‐ and intra‐plant level, thus establishing a method to provide high‐quality input for quantitative isotopic tracer studies.

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“…Within-plant variability and withinlabeling chamber variability are effectively pooled together in these analyses (Singh andColeman, 1974;Cheng et al, 1994). This procedure has often been used with continuous or multiple pulse-labeling, and when plant material was labeled for plant physiological or decomposition studies (Berg et al, 1991). In other studies, plants were rotated within the labeling chamber to reduce variability among plants due to position (Thompson, 1996;Rouhieretal., 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The labeling of plant materials with 13 C has been useful in producing large quantities of labeled plant material for decomposition studies (Berg et al, 1991) as well as in studies of plant C allocation during regrowth (Avice et al, 1996;Briske et al, 1996), but the sensitivity needed to trace C flows from plant roots into functional feeding groups of soil mesofauna is better met with 14 C.…”

Section: Introductionmentioning

confidence: 99%

Method for ¹⁴C‐labeling maize field plots and assessment of label uniformity within plots

Kisselle

Garrett

Hendrix

et al. 1999

Communications in Soil Science and Plant Analysis

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There is increasing interest in use of isotopic tracers to study nutrient liberation and transformation in plant tissues and soils. We developed a technique for pulse-labeling plants in the field with 14 C. Spatial distribution of radioactivity was measured in plots of maize (Zea mays L.) plants exposed to 14 CO 2 . Two clear polyvinyl chambers measuring 1 m wide x 2 m long x 1 m high were used to 14 C-label maize plants in conventional tillage and no-tillage treatments. A closed loop in-line with a pump allowed injection of 14 CO 2 and unlabeled CO 2 , and subsampling through an infrared gas analyzer. Cooling and mixing of the air within the chambers was achieved through the use of a free-standing automobile radiator with fan placed in the center of each plot. The specific

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A labeling chamber for 13C enrichment of plant tissue for decomposition studies

Cited by 22 publications

References 1 publication

The significance of below-ground fractions when considering N and C partitioning within chickpea (Cicer arietinum L.)

The significance of below-ground fractions when considering N and C partitioning within chickpea (Cicer arietinum L.)

Laser spectroscopy steered ¹³C‐labelling of plant material in a walk‐in growth chamber

Method for ¹⁴C‐labeling maize field plots and assessment of label uniformity within plots

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A labeling chamber for 13C enrichment of plant tissue for decomposition studies

Cited by 22 publications

References 1 publication

The significance of below-ground fractions when considering N and C partitioning within chickpea (Cicer arietinum L.)

The significance of below-ground fractions when considering N and C partitioning within chickpea (Cicer arietinum L.)

Laser spectroscopy steered 13C‐labelling of plant material in a walk‐in growth chamber

Method for 14C‐labeling maize field plots and assessment of label uniformity within plots

Contact Info

Product

Resources

About

Laser spectroscopy steered ¹³C‐labelling of plant material in a walk‐in growth chamber

Method for ¹⁴C‐labeling maize field plots and assessment of label uniformity within plots