A label-free graphene-based impedimetric biosensor for real-time tracing of the cytokine storm in blood serum; suitable for screening COVID-19 patients

Khayamian, Mohammad Ali; Parizi, Mohammad Salemizadeh; Ghaderinia, Mohammadreza; Abadijoo, Hamed; Vanaei, Shohreh; Simaee, Hossein; Abdolhosseini, Saeed; Shalileh, Shahriar; Faramarzpour, Mahsa; Naeini, Vahid Fadaei; Hoseinpour, Parisa; Shojaeian, Fatemeh; Abbasvandi, Fereshteh; Abdolahad, Mohammad

doi:10.1039/d1ra04298j

Cited by 12 publications

(7 citation statements)

References 55 publications

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“…al. [ 229 ] developed an electrochemical biosensor for cytokine storm tracking in COVID-19 patients using graphene-modified copper electrodes and EIS. It must be clarified that they were not interested in specific biomarker detection (only 50%) but in indicating disease severity with biomarkers of inflammation in blood samples, so they did not use bioreceptors in the detection.…”

Section: Monitoring Signatures Of Biomarkers With Biosensors In the C...mentioning

confidence: 99%

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Vásquez

Orozco

2022

Anal Bioanal Chem

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As a more efficient and effective way to address disease diagnosis and intervention, cutting-edge technologies, devices, therapeutic approaches, and practices have emerged within the personalized medicine concept depending on the particular patient's biology and the molecular basis of the disease. Personalized medicine is expected to play a pivotal role in assessing disease risk or predicting response to treatment, understanding a person's health status, and, therefore, health care decision-making. This work discusses electrochemical biosensors for monitoring multiparametric biomarkers at different molecular levels and their potential to elucidate the health status of an individual in a personalized manner. In particular, and as an illustration, we discuss several aspects of the infection produced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a current health care concern worldwide. This includes SARS-CoV-2 structure, mechanism of infection, biomarkers, and electrochemical biosensors most commonly explored for diagnostics, prognostics, and potentially assessing the risk of complications in patients in the context of personalized medicine. Finally, some concluding remarks and perspectives hint at the use of electrochemical biosensors in the frame of other cutting-edge converging/emerging technologies toward the inauguration of a new paradigm of personalized medicine. Graphical abstract

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Section: Monitoring Signatures Of Biomarkers With Biosensors In the C...mentioning

confidence: 99%

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Vásquez

Orozco

2022

Anal Bioanal Chem

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“…Graphene and its derivatives have been widely used as matrixes for the development of electrochemical biosensors [154][155][156][157][158][159] . Very recently, Khayamian et al 160 reported an impedimetric biosensor based on graphene for real-time monitoring the cytokine storm in serum which is suitable for screening COVID-19 patients. For this purpose, a graphene/copper working electrode on a sensor device was fabricated along with gold reference and gold electrodes.…”

Section: Nanostructuresmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

Robinson

Juska

O’Riordan

2022

Preprint

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Electrochemical Impedance Spectroscopy (EIS) is a surface sensitive technique which examines the impedance of an electrochemical cell over a range of frequencies. By immobilising an antigen or antibody to the electrode surface, EIS shows potential for highly specific and sensitive immunosensor performance. Following an introduction into antibodies and EIS, this review presents immobilisation techniques for an immunosensor’s bioreceptor. It highlights electrode modifications such as self-assembled monolayers (SAMs), polymers and nanostructures for anchoring the bioreceptor to the surface. Stable immobilisation directly to these modifications is not always possible. Immobilisation techniques to these modified surfaces are explored including cross-linking and controlled orientation. EIS allows for label-free detection by monitoring the change in charge-transfer resistance or capacitance caused by changes in the electrode surface. Label-free immunosensors are the focus of this paper as they show potential for point-of-care (POC) devices.

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“…Recently, as the COVID-19 pandemic unfolded, multiple emerging techniques for fast, label-free, noninvasive screening methods were developed. [1][2][3][4][5][6][7] However, given the urgency of the need to screen large numbers of people, more established techniques-including reverse transcription polymerase chain reaction (RT-PCR)-were widely used for viral detection in blood, nasopharyngeal swaps, but also in saliva. [8][9][10] Despite being generally highly sensitive, techniques such as RT-PCR are often timeintensive and require costly chemical reagents.…”

Section: Introductionmentioning

confidence: 99%

“…Human biofluids are commonly used in screening, therapeutic and diagnostic applications as they enable the direct measurement of circulating biomarkers related to a patient's health. Recently, as the COVID‐19 pandemic unfolded, multiple emerging techniques for fast, label‐free, noninvasive screening methods were developed 1–7 . However, given the urgency of the need to screen large numbers of people, more established techniques–including reverse transcription polymerase chain reaction (RT‐PCR)–were widely used for viral detection in blood, nasopharyngeal swaps, but also in saliva 8–10 .…”

Section: Introductionmentioning

confidence: 99%

Spectral effects and enhancement quantification in healthy human saliva with surface‐enhanced Raman spectroscopy using silver nanopillar substrates

Zamani,

Ksantini,

Sheehy

et al. 2023

Lasers Surg Med

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ObjectivesRaman spectroscopy as a diagnostic tool for biofluid applications is limited by low inelastic scattering contributions compared to the fluorescence background from biomolecules. Surface‐enhanced Raman spectroscopy (SERS) can increase Raman scattering signals, thereby offering the potential to reduce imaging times. We aimed to evaluate the enhancement related to the plasmonic effect and quantify the improvements in terms of spectral quality associated with SERS measurements in human saliva.MethodsDried human saliva was characterized using spontaneous Raman spectroscopy and SERS. A fabrication protocol was implemented leading to the production of silver (Ag) nanopillar substrates by glancing angle deposition. Two different imaging systems were used to interrogate saliva from 161 healthy donors: a custom single‐point macroscopic system and a Raman micro‐spectroscopy instrument. Quantitative metrics were established to compare spontaneous RS and SERS measurements: the Raman spectroscopy quality factor (QF), the photonic count rate (PR), the signal‐to‐background ratio (SBR).ResultsSERS measurements acquired with an excitation energy four times smaller than with spontaneous RS resulted in improved QF, PR values an order of magnitude larger and a SBR twice as large. The SERS enhancement reached 100×, depending on which Raman bands were considered.ConclusionsSingle‐point measurement of dried saliva with silver nanopillars substrates led to reproducible SERS measurements, paving the way to real‐time tools of diagnosis in human biofluids.

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A label-free graphene-based impedimetric biosensor for real-time tracing of the cytokine storm in blood serum; suitable for screening COVID-19 patients

Abstract: Rapid and label-free detection of the cytokine storm in COVID-19 patients by an electrochemical impedimetric biosensor with a graphene working electrode.

Cited by 12 publications

References 55 publications

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

Spectral effects and enhancement quantification in healthy human saliva with surface‐enhanced Raman spectroscopy using silver nanopillar substrates

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