A label-free fluorescence strategy for sensitive detection of ATP based on the ligation-triggered super-sandwich

Dual-Target Electrochemical Biosensing Based on DNA Structural Switching on Gold Nanoparticle-Decorated MoS2 Nanosheets

“…As expected, the electrochemical responses change (Δ I = I target – I no target ) of Fc was linear with the increasing ATP concentration in the range from 1 nM to 10 mM with the linear correlation coefficient of 0.999 (Figure A). The detection limit was calculated to be 0.32 nM (Figure B), which was comparable to other reports or better than some previous works. , More importantly, the increased ATP concentration had almost no influence on the electrochemical signal of MB. Similarly, the peak current of MB decreased with thrombin concentration increasing (Figure C).…”

“…The detection limit of ATP and thrombin was 0.74 nM and 0.0012 nM, respectively. A comparison of the present results with other nanomaterials-based aptasensors was listed in Table S1, suggesting that the analytical parameters including linear range and detection limit based on ATPA&TBA/AuNPs–MoS 2 /GCE were comparable to or better than the reported results. ,− All results confirmed that this proposed MoS 2 -based aptasensor could individually and simultaneously determine ATP and thrombin when they coexist in a buffer solution.…”

Fluorometric detection of mutant DNA oligonucleotide based on toehold strand displacement-driving target recycling strategy and exonuclease III-assisted suppression

“…10,000 Â SYBR Green I (SG) was provided by Sangon Biotech Co., Ltd., Shanghai, China. The as-received SG stocking solution was about 19.8 mM, based on the Lambert-Beer measurement (Lin et al, 2015). A 19.8 μM SG dyeing solution was obtained by diluting the stocking solution with dimethyl sulfoxide (DMSO) and stored in darkness at À20°C for use.…”

Labeling-free fluorescent detection of DNA hybridization through FRET from pyrene excimer to DNA intercalator SYBR green I

“…The labeling-free assays using DNA intercalators can exhibit high sensitivity and selectivity with low cost and convenient operation (Aied et al, 2012;Lin et al, 2015;Pu and Liu, 2009;Wang et al, 2011Wang et al, , 2014Xu et al, 2010). SYBR Green I (SG), a DNA intercalating dye, is an asymmetrical cyanine dye and has a significantly enhanced fluorescence in complex with dsDNA due to a dampening of its intra-molecular motions (Dragan et al, 2012), which can be potentially utilized to develop labeling-free sensors for DNA hybridization detection.…”