A label-free fluorescence assay for hydrogen peroxide and glucose based on the bifunctional MIL-53(Fe) nanozyme

Lin, Tianran; Qin, Yuemei; Huang, Yuanlin; Yang, Ruitao; Hou, Li; Ye, Fanggui; Zhao, Shulin

doi:10.1039/c7cc09819g

Cited by 122 publications

(64 citation statements)

References 42 publications

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“…The structure of MIL-53(Fe) was characterized first by XRD. As Figure 2A shows, particles of MIL-53(Fe) are highly crystalline, and the obvious sharp diffraction peaks correspond to the previously reported results as well as the simulated results (Lin et al, 2018 ). In addition, FTIR spectra was utilized for identifying the characteristic functional groups.…”

Section: Resultssupporting

confidence: 88%

Colorimetric Detection of Salicylic Acid in Aspirin Using MIL-53(Fe) Nanozyme

et al. 2020

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The impurity of salicylic acid (SA) in aspirin is a required inspection item for drug quality control. Since free SA is significantly toxic for humans, the content determination of free SA is absolutely necessary to ensure people's health. In this work, a facile colorimetric method was developed for the detection of SA in aspirin by utilizing the MIL-53(Fe) nanozyme. As MIL-53(Fe) possesses enzyme mimicking catalytic activity, 3,3,5,5-tetramethylbenzidine (TMB) can be easily oxidized to blue-oxidized TMB (oxTMB) with the existence of H 2 O 2. Moreover, an inhibition effect on the catalytic activity of the MIL-53(Fe) nanozyme is induced due to the specific complexation between SA and Fe 3+ in the center of MIL-53(Fe), which results in a lighter color in the oxTMB. The color change of oxTMB can be seen easily by the naked eye with the addition of different concentrations of SA. Thus, a simple colorimetric platform was established for effectively monitoring SA. A good linear relationship (R 2 = 0.9990) was obtained in the concentration range of 0.4-28 µmol L −1 , and the detection limit was 0.26 µmol L −1. In particular, the rationally designed system has been well-applied to the detection of SA impurity in aspirin. Satisfyingly, the detection results are highly in accord with those of HPLC. This novel colorimetric platform broadens the application prospects of nanozymes in the field of pharmaceutical analysis.

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Section: Resultssupporting

confidence: 88%

Colorimetric Detection of Salicylic Acid in Aspirin Using MIL-53(Fe) Nanozyme

et al. 2020

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“…For instance, Lin and co-workers synthesized MIL-53(Fe) nanozymes with enzyme-mimicking activity similar to HRP and capable of catalysing the oxidation of terephthalic acid (TA), a fluorescent probe for hydroxyl radicals (Fig. 7 b) [ 200 ]. Further working mechanism studies suggested that, nanozyme-assisted oxidation of TA lead to the production of 2-hydroxyterephthalic acid (TAOH) (a fluorescent product) which is being facilitated by the presence of H 2 O 2 , generated by the hydrolysis of glucose in the presence of GOx [ 200 ].…”

Section: Nanozymes For the Development Of Biosensors And Poc Devices Following Assured Criteriamentioning

confidence: 99%

Nanozymes in Point-of-Care Diagnosis: An Emerging Futuristic Approach for Biosensing

et al. 2021

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Nanomaterial-based artificial enzymes (or nanozymes) have attracted great attention in the past few years owing to their capability not only to mimic functionality but also to overcome the inherent drawbacks of the natural enzymes. Numerous advantages of nanozymes such as diverse enzyme-mimicking activities, low cost, high stability, robustness, unique surface chemistry, and ease of surface tunability and biocompatibility have allowed their integration in a wide range of biosensing applications. Several metal, metal oxide, metal–organic framework-based nanozymes have been exploited for the development of biosensing systems, which present the potential for point-of-care analysis. To highlight recent progress in the field, in this review, more than 260 research articles are discussed systematically with suitable recent examples, elucidating the role of nanozymes to reinforce, miniaturize, and improve the performance of point-of-care diagnostics addressing the ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free and deliverable to the end user) criteria formulated by World Health Organization. The review reveals that many biosensing strategies such as electrochemical, colorimetric, fluorescent, and immunological sensors required to achieve the ASSURED standards can be implemented by using enzyme-mimicking activities of nanomaterials as signal producing components. However, basic system functionality is still lacking. Since the enzyme-mimicking properties of the nanomaterials are dictated by their size, shape, composition, surface charge, surface chemistry as well as external parameters such as pH or temperature, these factors play a crucial role in the design and function of nanozyme-based point-of-care diagnostics. Therefore, it requires a deliberate exertion to integrate various parameters for truly ASSURED solutions to be realized. This review also discusses possible limitations and research gaps to provide readers a brief scenario of the emerging role of nanozymes in state-of-the-art POC diagnosis system development for futuristic biosensing applications.

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“…Lin et al proposed an enzyme based platform for the detection of this carbohydrate. The sensing system was constructed by using the label-free MIL-53(Fe) nanozyme and glucose oxidase [ 104 ]. Occurring enzymatic reactions cause changes in fluorescence intensity, which correspond to particular glucose concentrations.…”

Section: Fluorescent Biosensors For Determination Of Key Metabolitmentioning

confidence: 99%

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

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Drzozga

Baluta

et al. 2018

Sensors

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The clinical applications of sensing tools (i.e., biosensors) for the monitoring of physiologically important analytes are very common. Nowadays, the biosensors are being increasingly used to detect physiologically important analytes in real biological samples (i.e., blood, plasma, urine, and saliva). This review focuses on biosensors that can be applied to continuous, time-resolved measurements with fluorescence. The material presents the fluorescent biosensors for the detection of neurotransmitters, hormones, and other human metabolites as glucose, lactate or uric acid. The construction of microfluidic devices based on fluorescence uses a variety of materials, fluorescent dyes, types of detectors, excitation sources, optical filters, and geometrical systems. Due to their small size, these devices can perform a full analysis. Microfluidics-based technologies have shown promising applications in several of the main laboratory techniques, including blood chemistries, immunoassays, nucleic-acid amplification tests. Of the all technologies that are used to manufacture microfluidic systems, the LTCC technique seems to be an interesting alternative. It allows easy integration of electronic and microfluidic components on a single ceramic substrate. Moreover, the LTCC material is biologically and chemically inert, and is resistant to high temperature and pressure. The combination of all these features makes the LTCC technology particularly useful for implementation of fluorescence-based detection in the ceramic microfluidic systems.

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A label-free fluorescence assay for hydrogen peroxide and glucose based on the bifunctional MIL-53(Fe) nanozyme

Cited by 122 publications

References 42 publications

Colorimetric Detection of Salicylic Acid in Aspirin Using MIL-53(Fe) Nanozyme

Colorimetric Detection of Salicylic Acid in Aspirin Using MIL-53(Fe) Nanozyme

Nanozymes in Point-of-Care Diagnosis: An Emerging Futuristic Approach for Biosensing

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

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