Colorimetric Detection of Salicylic Acid in Aspirin Using MIL-53(Fe) Nanozyme

Abstract

The impurity of salicylic acid (SA) in aspirin is a required inspection item for drug quality control. Since free SA is significantly toxic for humans, the content determination of free SA is absolutely necessary to ensure people's health. In this work, a facile colorimetric method was developed for the detection of SA in aspirin by utilizing the MIL-53(Fe) nanozyme. As MIL-53(Fe) possesses enzyme mimicking catalytic activity, 3,3,5,5-tetramethylbenzidine (TMB) can be easily oxidized to blue-oxidized TMB (oxTMB) with the existence of H 2 O 2. Moreover, an inhibition effect on the catalytic activity of the MIL-53(Fe) nanozyme is induced due to the specific complexation between SA and Fe 3+ in the center of MIL-53(Fe), which results in a lighter color in the oxTMB. The color change of oxTMB can be seen easily by the naked eye with the addition of different concentrations of SA. Thus, a simple colorimetric platform was established for effectively monitoring SA. A good linear relationship (R 2 = 0.9990) was obtained in the concentration range of 0.4-28 µmol L −1 , and the detection limit was 0.26 µmol L −1. In particular, the rationally designed system has been well-applied to the detection of SA impurity in aspirin. Satisfyingly, the detection results are highly in accord with those of HPLC. This novel colorimetric platform broadens the application prospects of nanozymes in the field of pharmaceutical analysis.

Recent advances in colorimetric sensors based on nanozymes with peroxidase-like activity

“…Furthermore, MIL-53(Fe) was chosen as MOFs-based nanozymes, serving to realize the colorimetric detection of salicylic acid (SA) in aspirin. 148 MIL-53(Fe) prepared using a solvothermal method exhibited favorable peroxidase-mimicking activity, which catalyzed the oxidation of TMB in the presence of H 2 O 2 and rendered it blue. Notably, the catalytic activity of MIL-53(Fe) was clearly suppressed after the introduction of SA, which displayed a lighter blue color due to the complexation reaction of SA with Fe 3+ at the center of MIL-53(Fe) (Fig.…”

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

“…The change in the visible color is attributed to the catalytic reaction between enzymes and chromogenic substrates. 3,3 ,5,5 -Tetramethylbenzidine (TMB) is one of the most widely used chromogens [94,95]. It is oxidized by peroxidase in the presence of H 2 O 2 and undergoes color change from colorlessness to blue.…”

Sustainable agriculture with LEAFS: a low-cost electrochemical analyzer of foliage stress

“…Traditionally, the detection and quantification of salicylic acid have been accomplished through methods such as liquid chromatography, 8–10 gas chromatography, 11,12 colorimetry, 13–16 and electrokinetic chromatography. 17 Although they have good reliability, these techniques often require large equipment, complicated sample preparation, and can be time-consuming.…”