A Label-Free Colorimetric Assay Based on Gold Nanoparticles for the Detection of H2O2 and Glucose

Abstract

The significance of sensing hydrogen peroxide (H2O2) is due to its ubiquity, being a potential biomarker as well as an end-product of several oxidation reactions. Herein, based on gold nanoparticles (AuNPs) and coupled with single-stranded DNA (ssDNA) and ceria nanoparticles (CeO2), we developed a novel colorimetric method to detect H2O2 and glucose in NaCl solutions. In the presence of H2O2, ssDNA adsorbed on the surface of CeO2 could be released and subsequently decorated AuNPs, resulting in a distinct color change of the aqueous solution from purple to red, which could be observed by the naked eye. Since H2O2 can be produced in the process of glucose oxidation by glucose oxidase (GOx), this approach can also be employed to detect glucose. By employing this sensing system, the detection limits for H2O2 and glucose are about 0.21 μM and 3.01 µM, respectively. Additionally, monitoring the content of glucose in blood serum samples was successfully achieved by the proposed strategy. This work opens a potential avenue for the quantitative detection of H2O2 and glucose in clinical diagnostics.

A Novel Method for the Pre-Column Derivatization of Saccharides from Polygonatum cyrtonema Hua. by Integrating Lambert–Beer Law and Response Surface Methodology

“…However, the direct test's sensitivity and accuracy are low [9]. By comparison, colorimetric chemosensors have received increased attention due to their excellent sensitivity and operational simplicity [10][11][12].…”

Metalloporphyrin-Based Metal–Organic Framework Nanorods for Peroxidase-Like Catalysis

“…Hydrogen peroxide (H 2 O 2 ) plays a key role in many biological processes, and its concentration reflects the condition of cells, tissues, and even diseases. , H 2 O 2 is also employed as a bleaching agent and stabilizer in the food industry for the treatment and packaging of different food products, including milk and juice; , however, residual H 2 O 2 can damage human cells and cause diseases. , Thus, rapid, accurate, and low-cost determination of H 2 O 2 is highly desired in various fields, such as food safety, biological analyses, and environmental monitoring. Electrochemical techniques, fluorescence spectroscopy, and liquid chromatography exhibit good sensing performances. , In addition, the colorimetric method is a promising alternative for detecting H 2 O 2 because of its low cost, easy operation, and high practicability. , In small-molecule sensing, enzyme-mimicking nanomaterials can overcome the inherent problems of natural enzymes, such as specific storage/use conditions and low stabilities . Nonenzymatic materials, such as noble-metal nanoparticles (Au, Ag, and Pt and their composites), transition-metal oxides and disulfides (Fe 3 O 4 , MoS 2 , and WS 2 ), and single-atom materials (atomically dispersed Co and Fe sites), are used as catalysts for the adsorption/conversion of target molecules, thereby amplifying the sensing response. − However, a few nonenzymatic materials exhibited lower sensing performances than natural enzymes.…”

“…7,8 In addition, the colorimetric method is a promising alternative for detecting H 2 O 2 because of its low cost, easy operation, and high practicability. 9,10 In small-molecule sensing, enzyme-mimicking nanomaterials can overcome the inherent problems of natural enzymes, such as specific storage/use conditions and low stabilities. 11 Nonenzymatic materials, such as noble-metal nanoparticles (Au, Ag, and Pt and their composites), transition-metal oxides and disulfides (Fe 3 O 4 , MoS 2 , and WS 2 ), and single-atom materials (atomically dispersed Co and Fe sites), are used as catalysts for the adsorption/conversion of target molecules, thereby amplifying the sensing response.…”