A label-free and PCR-free electrochemical assay for multiplexed microRNA profiles by ligase chain reaction coupling with quantum dots barcodes

Zhu, Wenyuan; Su, Xingpeng; Gao, Xiaoyu; Dai, Zong; Zou, Xiaoyong

doi:10.1016/j.bios.2013.10.023

Cited by 96 publications

(38 citation statements)

References 31 publications

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“…The detection limit of this method was 0.32 aM with a six-decade dynamic range. Furthermore, Zhu et al [66] developed a label-free and PCR-free electrochemical biosensor for multiplexed assay of miRNAs based on the combination of the high base-mismatch selectivity of ligase chain reaction (LCR) and the remarkable voltammetric signature of electrochemical QDs barcodes. In the work, PbS and CdS QDs were labeled with the report probes of RP1 and RP2, respectively, to form the PbS-RP1 and CdS-RP2 conjugates.…”

Section: Nanomaterials-based Electrochemical Strategies For Mirnasmentioning

confidence: 99%

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Xia

Zhang

2014

Materials

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MicroRNAs (miRNAs) play important functions in post-transcriptional regulation of gene expression. They have been regarded as reliable molecular biomarkers for many diseases including cancer. However, the content of miRNAs in cells can be low down to a few molecules per cell. Thus, highly sensitive analytical methods for miRNAs detection are desired. Recently, electrochemical biosensors have held great promise as devices suitable for point-of-care diagnostics and multiplexed platforms for fast, simple and low-cost nucleic acid analysis. Signal amplification by nanomaterials is one of the most popular strategies for developing ultrasensitive assay methods. This review surveys the latest achievements in the use of nanomaterials to detect miRNAs with a focus on electrochemical techniques.

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Section: Nanomaterials-based Electrochemical Strategies For Mirnasmentioning

confidence: 99%

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Xia

Zhang

2014

Materials

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“…The first one was presented by Wu et al, 2013, employing a conductive composition of Nafion, thionine (Thi) and Pd nanoparticles, displaying electrocatalytic activity for H 2 O 2 , to provide an LOD of ~1900 fM. Zhu et al, 2014, combined high base-mismatch selectivity of ligase chain reaction with reporting probes labeled with two different quantum dots. This elegant and complex approach had an LOD ranging from 12 to 31 fM.…”

Section: Introductionmentioning

confidence: 99%

Novel and simple electrochemical biosensor monitoring attomolar levels of miRNA-155 in breast cancer

Cardoso

Moreira

Fernandes

et al. 2016

Biosensors and Bioelectronics

151

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This work, describes for the first time, a simple biosensing design to yield an ultrasensitive electrochemical biosensor for a cancer biomarker detection, miRNA-155, with linear response down to the attomolar range. MiRNA-155 was selected for being overexpressed in breast cancer. The biosensor was assembled in two stages: (1) the immobilization of the anti-miRNA-155 that was thiol modified on an Au-screen printed electrode (Au-SPE), followed by (2) blocking the areas of non-specific binding with mercaptosuccinic acid. Atomic force microscopy (AFM) and electrochemical techniques including cyclic voltammetry (CV), impedance spectroscopy (EIS) and square wave voltammetry (SWV) confirmed the surface modification of these devices and their ability to hybridize successfully and stably with miRNA-155. The final biosensor provided a sensitive detection of miRNA-155 from 10 aM to 1.0 nM with a low detection limit (LOD) of 5.7 aM in real human serum samples. Good results were obtained in terms of selectivity towards breast cancer antigen CA-15.3 and bovine serum albumin (BSA). Raw fluid extracts from cell-lines of melanoma did not affect the biosensor response (no significant change of the blank), while raw extracts from breast cancer yielded a positive signal against miRNA-155. This simple and sensitive strategy is a promising alternative for simultaneous quantitative analysis of multiple miRNA in physiological fluids for biomedical research and point-of-care (POC) diagnosis.

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“…EXPAR has the intrinsic merits of isothermal nature, high amplification efficiency and rapid amplification kinetics, which can provide 10 6 -10 9 fold amplification within minutes under isothermal conditions. In addition, metal nanoparticles (Sharifi et al, 2014;Wu et al, 2014), quantum dots (Zhu et al, 2014), DNA biobarcode (Xu et al, 2012;Zhou et al, 2014b) and labeled enzymes (Ge et al, 2014) were also the common examples of amplification strategy. However, the multiple assay steps, the addition of many exogenous reagents and the labeling process made them laborious and costly.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive electrochemical detection of avian influenza A (H7N9) virus DNA based on isothermal exponential amplification coupled with hybridization chain reaction of DNAzyme nanowires

Chen

Jian

et al. 2015

Biosensors and Bioelectronics

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A label-free and PCR-free electrochemical assay for multiplexed microRNA profiles by ligase chain reaction coupling with quantum dots barcodes

Cited by 96 publications

References 31 publications

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Novel and simple electrochemical biosensor monitoring attomolar levels of miRNA-155 in breast cancer

Ultrasensitive electrochemical detection of avian influenza A (H7N9) virus DNA based on isothermal exponential amplification coupled with hybridization chain reaction of DNAzyme nanowires

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