A Kinetic Study of Concanavalin A Binding to Glycolipid Monolayers by Using a Quartz-Crystal Microbalance

Ebara, Yasuhito; Okahata, Yoshio

doi:10.1021/ja00104a001

Cited by 163 publications

(106 citation statements)

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“…A QCM is known to provide very sensitive mass measuring devices in gas phase [6][7][8][9][10][11] and in aqueous solution. [15][16][17][18][19][20][21][22][23] Its resonance frequency decreases linearly upon the increase of mass on the QCM electrode at the nanogram level. When ssDNA is immobilized on a QCM, the hybridization behavior could be followed as a frequency decrease (mass increase) responding to the addition of complementary ssDNA in the solution.…”

Section: Introductionmentioning

confidence: 99%

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A Highly Sensitive 27 MHz Quartz-Crystal Microbalance as a Device for Kinetic Measurements of Molecular Recognition on DNA Strands

et al. 2000

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DNA-DNA hybridization and DNA-protein or enzyme interactions are essential steps in biomolecular recognitions. These interactions have been conventionally studied by gel mobility shift assay. This technique gives us only qualitative information, we must label enzymes radioactive or fluorescent molecules, and it takes a relatively long time to analyze the results. Here we introduce a new tool of a highly sensitive 27 MHz quartz-crystal microbalance (QCM), in which the resonance frequency decreases linearly with the increase of mass on the electrode area at the nanogram level. Thus, when a host molecule is immobilized on a QCM, the binding behavior and its kinetics can be monitored from frequency changes due to the guest binding in aqueous solution. The results are also compared with those from a surface plasmon resonance sensor.

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Section: Introductionmentioning

confidence: 99%

“…Therefore, we calibrated our cased 27 MHz QCM in aqueous solution (pH 7.9, 10 mM Tris-HCl, 0.2 M NaCl, at 20˚C), analogously to the calibration of our conventional 9 MHz QCM. [18][19][20][21][22][23] A suitable amount of polymer solution was cast (0.1 -15 mg…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive 27 MHz Quartz-Crystal Microbalance as a Device for Kinetic Measurements of Molecular Recognition on DNA Strands

et al. 2000

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“…When a mass is adsorbed on the resonator there is a shift in resonant frequency, which is related to the adsorbed mass by the Sauerbrey equation [30]. According to this relationship, the change in frequency (ΔF, Hz) is related to total mass of the adsorbed thin film layer (Δm, ng) as Δm = −0.87ΔF.…”

Section: Assembly On Qcm Resonatormentioning

confidence: 99%

“…Thus, we aim to build precise nanoassemblies of protein/polysaccharide multilayers to control the total sample per unit area, by exploiting the multiple binding sites of Con A and the multiple glucose groups of dextran [27,28]. It was hypothesized that this assembly approach would be successful based on the previous work done on multilayer films of Con A/glycogen [29] and Con A/glycolipids [30] built due to affinity between the molecules and not due to the electrostatic attractions.…”

Section: Introductionmentioning

confidence: 99%

Glucose-Sensitive Nanoassemblies Comprising Affinity-Binding Complexes Trapped in Fuzzy Microshells

Chinnayelka

McShane

2004

Journal of Fluorescence

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A new design for glucose monitoring with "smart" materials based on self assembly, competitive binding, and resonance energy transfer (RET) is presented. The basic transduction principle is changing RET efficiency from fluorescein isothiocyanate (FITC) to tetramethylrhodamine isothiocyanate (TRITC), as FITC-dextran is displaced from TRITC-Concanavalin A (Con A) with the addition of glucose. Nanoscale fabrication by self-assembly of Con A/dextran into multilayer films, followed by polymer multilayers. The advantages of this approach include physical localization and separation of sensing molecules from the environment via entrapment of the biosensor elements in a semi-permeable polymeric shell, and only functional molecules are included in the sensors. To realize these nanostructures, dissolvable resin microparticles were coated with FITC-dextran+TRITC-Con A multilayers, followed by polyelectrolyte multilayers, and the core particles were then dissolved to yield hollow capsules. The nanoassembly process was studied using microbalance mass measurements, fluorescence spectroscopy, confocal fluorescence microscopy, and zeta-potential measurements. The key findings are that the specific binding between Con A and dextran can be used to deposit ultrathin multilayer films, and these exhibit changing RET in response to glucose. Fluorescence spectra of a microcapsules exhibited a linear, glucose-specific, 27% increase in the relative fluorescence of FITC over the 0-1800 mg/dL range. These findings demonstrate the feasibility of using self-assembled microcapsules as optical glucose sensors, and serve as a basis for work toward better understanding the properties of these novel materials.

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“…Other methods to map the interactions, closely matching that of two-dimensional cell surfaces, are the Langmuir [6], the surface plasmon resonance (SPR) [7] and quartz crystal microbalance (QCM) methods [8,9] that allow the characterization of kinetic parameters, in addition to deriving equilibrium binding constants. Fluorescence based assays were also used in cases when either a fluorophore or chromophore engages in a reporter role of the recognition events [10].…”

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Detection of sugar-lectin interactions by multivalent dendritic sugar functionalized single-walled carbon nanotubes

et al. 2012

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Abstract:We show that single walled carbon nanotubes (SWNT) decorated with sugar functionalized poly (propyl ether imine) (PETIM) dendrimer is a very sensitive platform to quantitatively detect . The increase in the device conductance observed after adding 10 nM of Con A is same as after adding 20 µM of a non -specific lectin peanut agglutinin, showing the high specificity of the Con A -mannose interactions. The specificity of sugar-lectin interactions was characterized further by observing significant shifts in Raman modes of the SWNT.

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A Kinetic Study of Concanavalin A Binding to Glycolipid Monolayers by Using a Quartz-Crystal Microbalance

Cited by 163 publications

References 0 publications

A Highly Sensitive 27 MHz Quartz-Crystal Microbalance as a Device for Kinetic Measurements of Molecular Recognition on DNA Strands

A Highly Sensitive 27 MHz Quartz-Crystal Microbalance as a Device for Kinetic Measurements of Molecular Recognition on DNA Strands

Glucose-Sensitive Nanoassemblies Comprising Affinity-Binding Complexes Trapped in Fuzzy Microshells

Detection of sugar-lectin interactions by multivalent dendritic sugar functionalized single-walled carbon nanotubes

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