Detection of sugar-lectin interactions by multivalent dendritic sugar functionalized single-walled carbon nanotubes

Vasu, K.I.; Kumar, Naresh; Bagul, Rahul S.; Jayaraman, Narayanaswamy; Sood, Anil K.

doi:10.1063/1.4739793

Cited by 13 publications

(7 citation statements)

References 29 publications

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“…This is probably due to increased carrier scattering and/or charge transfer from the electron donating amino groups of the target CRP analyte upon its binding to anti-CRP on the SWNT walls of FET device, as reported earlier. 34 On the other hand, the exposure to pure PBS did not produce much change in the source-drain current (inset of Fig. 2(d): upper-right side).…”

mentioning

confidence: 92%

High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

Rajesh

Sharma

Puri

et al. 2016

Applied Physics Letters

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We report a single-walled carbon nanotube (SWNT) field-effect transistor (FET) functionalized with Polyamidoamine (PAMAM) dendrimer with 128 carboxyl groups as anchors for site specific biomolecular immobilization of protein antibody for C-reactive protein (CRP) detection. The FET device was characterized by scanning electron microscopy and current-gate voltage (I-Vg) characteristic studies. A concentration-dependent decrease in the source-drain current was observed in the regime of clinical significance, with a detection limit of ∼85 pM and a high sensitivity of 20% change in current (ΔI/I) per decade CRP concentration, showing SWNT being locally gated by the binding of CRP to antibody (anti-CRP) on the FET device. The low value of the dissociation constant (Kd = 0.31 ± 0.13 μg ml−1) indicated a high affinity of the device towards CRP analyte arising due to high anti-CRP loading with a better probe orientation on the 3-dimensional PAMAM structure.

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confidence: 92%

High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

Rajesh

Sharma

Puri

et al. 2016

Applied Physics Letters

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“…Both devices could detect lectins down to low nM level and could provide K D values of their interaction with immobilized glycans 120. CNT-modified FET-device with glycan immobilized via dendrimer was also applied in detection of Con A down to 10 nM 127. Silicon nanowire-based FET glycan device was prepared by immobilization of natural glycan on a surface patterned by amino-oxy functional group 50.…”

Section: Application Of Glycan Biosensorsmentioning

confidence: 99%

Are glycan biosensors an alternative to glycan microarrays?

Hushegyi

Tkac

2014

Anal. Methods

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Complex carbohydrates (glycans) play an important role in nature and study of their interaction with proteins or intact cells can be useful for understanding many physiological and pathological processes. Such interactions have been successfully interrogated in a highly parallel way using glycan microarrays, but this technique has some limitations. Thus, in recent years glycan biosensors in numerous progressive configurations have been developed offering distinct advantages compared to glycan microarrays. Thus, in this review advances achieved in the field of label-free glycan biosensors are discussed.

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“…In recent times, HbA 1c detection using boronic acid and its derivatives has been attracting a lot of attention from the scientific community due to the ability of formation of the boronate ester bond through 1,2‐diol or 1,3‐diol . On the other hand, carbon nanomaterials such as single‐walled carbon nanotubes (SWNTs), graphene and its derivatives are well known for their applications in developing biosensors , particularly in enzymatic and nonenzymatic glucose sensing with high sensitivity due to their excellent optical and electronic transport properties. Carbon nanomaterials have also been used in the development of enzymatic and nonenzymatic glucose sensors with high sensitivity and stability.…”

Section: Introductionmentioning

confidence: 99%

Optical detection of glucose and glycated hemoglobin using etched fiber Bragg gratings coated with functionalized reduced graphene oxide

Sridevi

Vasu

Sampath

et al. 2015

Journal of Biophotonics

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An enhanced optical detection of D-glucose and glycated hemoglobin (HbA1c ) has been established in this study using etched fiber Bragg gratings (eFBG) coated with aminophenylboronic acid (APBA)-functionalized reduced graphene oxide (RGO). The read out, namely the shift in Bragg wavelength (ΔλB ) is highly sensitive to changes that occur due to the adsorption of glucose (or HbA1c ) molecules on the eFBG sensor coated with APBA-RGO complex through a five-membered cyclic ester bond formation between glucose and APBA molecules. A limit of detection of 1 nM is achieved with a linear range of detection from 1 nM to 10 mM in the case of D-glucose detection experiments. For HbA1c , a linear range of detection varying from 86 nM to 0.23 mM is achieved. The observation of only 4 pm (picometer) change in ΔλB even for the 10 mM lactose solution confirms the specificity of the APBA-RGO complex coated eFBG sensors to glucose molecules.

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Detection of sugar-lectin interactions by multivalent dendritic sugar functionalized single-walled carbon nanotubes

Cited by 13 publications

References 29 publications

High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

Are glycan biosensors an alternative to glycan microarrays?

Optical detection of glucose and glycated hemoglobin using etched fiber Bragg gratings coated with functionalized reduced graphene oxide

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