A kinetic model for the quantitative analysis of complement

Pruitt, Kenneth M.; Turner, Malcolm E.; Boackle, Robert J.

doi:10.1016/0022-5193(74)90157-x

Cited by 16 publications

(1 citation statement)

References 12 publications

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“…During an assay there is first a lag phase with an increase of the OD signal due to internal kinetic movements inside the liquid phase that lasts for about 10 s followed by a stabilization period of variable length, then a second sigmoidal phase due to hemolysis [4], Figure 2 shows the OD versus time data dis played on the screen during a typical assay and superimposed is the first derivative curve dOD/dt.…”

Section: Different Modes For Determination Of Half Hemolysismentioning

confidence: 99%

Computer-Assisted Kinetic Assay for Quantification of Total Complement Activity

Abbal

Tkaczuk²,

Praud³

et al. 1991

Complement Inflamm

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Using apparatus available in any laboratory we developed a semiautomated kinetic technique for complement activity assay. Hemolysis of sensitized red blood cells is performed in the thermostated microflow cell of a spectrophotometer connected to a computer. The computer controls, displays on the screen, and analyzes all the different phases of the assay. After definition of optimal operating conditions, we compared the results obtained by this technique and by Kabat and Mayer’s. On 221 patients’ sera the regression coefficient was 0.94. The values for samples deficient in one fraction or after in vitro activation were very similar. The coefficient of variation was close to 1 % for within series studies and better than 3% between series. This technique is very easy to perform even in a routine nonspe- cialized laboratory and up to 30-40 sample/h can be tested.

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Section: Different Modes For Determination Of Half Hemolysismentioning

confidence: 99%

Computer-Assisted Kinetic Assay for Quantification of Total Complement Activity

Abbal

Tkaczuk²,

Praud³

et al. 1991

Complement Inflamm

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The Partial Isolation and Function of Salivary Factors Which Interact with the Complement System: a Possible Role in Mucosal Immunity

Boackle

Caughman

Carsgo

1978

Advances in Experimental Medicine and Biology

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Optimal strategies in immunology III. The IgM-IgG switch

1980

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During a primary immune response generally two classes of antibody are produced, immunoglobulin M (IgM) and immunoglobulin G (IgG). It is currently thought that some lymphocytes which initially produce IgM switch to the production of IgG with the same specificity for antigen. During a secondary immune response IgG is the predominant antibody made throughout the response. In this paper we address the question of why such apparently complicated modes of response should have been adapted by evolution. We construct mathematical models of the immune response to growing antigens which incorporate complement dependent cell lysis. By comparing the times required to eliminate antigen we show that under certain conditions it is advantageous for an animal to switch some of its lymphocytes from IgM to IgG production during a primary response, but yet to secrete only IgG during a secondary response. The sensitivity of such a conclusion to parameter variations is studied and the biological basis and implications of our models are fully discussed.

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A kinetic model for the quantitative analysis of complement

Cited by 16 publications

References 12 publications

Computer-Assisted Kinetic Assay for Quantification of Total Complement Activity

Computer-Assisted Kinetic Assay for Quantification of Total Complement Activity

The Partial Isolation and Function of Salivary Factors Which Interact with the Complement System: a Possible Role in Mucosal Immunity

Optimal strategies in immunology III. The IgM-IgG switch

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