A kinetic model for evaluation of the antioxidant activity of several rosemary extracts

Terpinc, Petra; Bezjak, Miran; Abramovič, Helena

doi:10.1016/j.foodchem.2008.12.033

Cited by 73 publications

(56 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One of them is the kinetic analysis with explicit mathematical equations. In this sense, we have found three groups of alternatives in the literature (Murado and Vázquez 2010;Özilgen and Özilgen 1990;Terpinc, Bezjak, and Abramovič 2009), covering a wide spectrum of profile responses, from potential to sigmoid ones, with and without intercepts. These mathematical tools facilitates the accurate prediction of the asymptotic end point value of the kinetic part of the response for all the doses assessed.…”

Section: Single Value To Assess the Response Of Antioxidantsmentioning

confidence: 97%

An efficient methodology for quantification of synergy and antagonism in single electron transfer antioxidant assays

Prieto

Curran

Gowen

et al. 2015

Food Research International

View full text Add to dashboard Cite

The development of new antioxidant compounds for incorporation in foods is a rapidly growing research area. The resulting interactions between complex antioxidant mixtures is a key issue, however, research in this area is still in its infancy. Experimental antioxidant models based on conventional dose-responses, that can predict joint effects of chemical mixtures, are urgently needed. This paper illustrates a methodological procedure for Single Electron Transfer (SET) antioxidant assays to determine the synergistic and antagonistic effects of combining binary mixtures of antioxidants. Despite the abundance of theories and procedures to describe the synergistic/antagonistic effects in SET assays, they appear to be inadequate. Some features hindering advances in this field include the lack of: (1) experimental design, as a result of the extended use of unambiguous and simplistic procedures to quantify the effects of joint responses, based on singledose values; (2) detailed mathematical hypotheses to quantify dose-response values, which in addition causes the associated difficulties for assessing the statistical consistence of the results; and (3) functional approaches that consider the possibility of interactive effects. This paper proposes solutions for each of these limitations. Established ideas from existing fields are used to replace the current simplistic procedures, in order to quantify the effects of joint responses. One of the common hypothesis (known as concentration addition) for describing the combined effects is established for SET assays. A dose dependent mathematical model representative of this hypothesis, based on probability functions with meaningful parameters, is applied. The interactive effects between antioxidants are introduced into the model with simple auxiliary functions that describe the variations induced by each antioxidant in the parameters that define the effects of the other. Finally, a comprehensive index to summarize the complex parametric responses in one single value is proposed. Although the approach was experimentally demonstrated just in two classical SET assays (DPPH and ABTS), the results could be directly expanded in future to other types of classical SET assays. The methodology proposed is more complex than some relatively common approaches; nevertheless we believe that it is free of the controversial aspects listed above. Statistically consistent responses of null, synergy and antagonism effects were found when characterizing the interactions between several pairs of individual and complex mixtures of chemical antioxidant agents.

show abstract

Section: Single Value To Assess the Response Of Antioxidantsmentioning

confidence: 97%

An efficient methodology for quantification of synergy and antagonism in single electron transfer antioxidant assays

Prieto

Curran

Gowen

et al. 2015

Food Research International

View full text Add to dashboard Cite

show abstract

“…The substances related with its antioxidant activity are phenolic diterpenes such as carnosol, rosmanol, carnosic acid, methyl carnosate, and phenolic acids such as rosmarinic and caffeic acids. Particularly, carnosic acid and carnosol are the most abundant antioxidants present in rosemary extracts [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products

Vázquez

García-Risco

Jaime

et al. 2013

The Journal of Supercritical Fluids

View full text Add to dashboard Cite

Esta es la versión de autor del artículo publicado en: This is an author produced version of a paper published in: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 1 Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of productsErika Vázquez, Mónica R. García-Risco, Laura Jaime, Guillermo Reglero and Tiziana Fornari* Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM).CEI UAM+CSIC. C/Nicolás Cabrera 9, Universidad Autónoma de Madrid, 28049 Madrid, Spain. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 *Corresponding author: CIAL (CSIC-UAM AbstractMixed vegetal extracts are interesting target of new products as nutraceuticals, superior ingredients for the design of functional food, singular ingredients for cosmetics, etc. In this work the extraction of a mixture of spinach and rosemary leaves (50 % weight of each plant)was investigated in terms of its antioxidant activity, and compared with the extraction of the separate species. Phenolic diterpenes of rosemary and carotenoids of spinach were target compounds due their recognized biological activities. Two different extraction techniques were applied, namely pressurized liquid extraction using hexane at two different temperatures (100 and 150C) and supercritical fluid extraction with pure carbon dioxide at 40C and two different pressures (20 and 30 MPa). For each extraction technique and conditions three different raw materials were employed: spinach leaves, rosemary leaves and the mixture 50:50 of spinach and rosemary leaves.The antioxidant activity of the samples produced was studied in terms of the ABTS assay and showed to be enhanced when the species are simultaneously extracted, with TEAC values around 20% higher than the values corresponding to mixing the extracts obtained by separate.A possible synergic effect between carotenoids and phenolic diterpenes was studied, although no specific synergic activity could be observed. However, the enhanced antioxidant activity could be attributed to a definite increase of the concentration of carnosic acid, which was observed in the samples produced by the simultaneous extraction.Keywords: Supercritical fluid extraction; Pressurized liquid extraction; Antioxidants; Phenolic diterpenes; Carotenoids; Spinach; Rosemary . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 3 IntroductionAntioxidants play a very important role in the food, cosmetic and pharmac...

show abstract

“…The main substances associated with the antioxidant activity are the phenolic diterpenes such as carnosol, rosmanol, carnosic acid, and methyl carnosate, and phenolic acids such as rosmarinic and caffeic acid [6][7][8][9][10]. Particularly, carnosic acid has been recognized as the most abundant antioxidant compound present in rosemary extracts.…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fractionation of Rosemary Extracts to Improve the Antioxidant Activity

Vicente¹,

García-Risco²,

Fornari³

et al. 2011

Chem Eng & Technol

View full text Add to dashboard Cite

A supercritical CO 2 pilot plant was employed to extract rosemary (Rosmarinus officinalis L.) leaves and to thoroughly study the effect of time on the yield, composition and antioxidant activity of the different fractions produced. Six extraction assays were carried out, varying the fractionation procedure using a twostage depressurization system coupled to the extractor vessel. The concentration of the main antioxidant compound in rosemary, i.e. carnosic acid, and the DPPH test were employed to evaluate the antioxidant power of the different fractions obtained. The goal was to develop a new fractionation scheme comprising two different periods of time, in which exhaustive extraction of the essential oil from the plant matrix was achieved while recovery of the antioxidant compounds was maximized.

show abstract

A kinetic model for evaluation of the antioxidant activity of several rosemary extracts

Cited by 73 publications

References 20 publications

An efficient methodology for quantification of synergy and antagonism in single electron transfer antioxidant assays

An efficient methodology for quantification of synergy and antagonism in single electron transfer antioxidant assays

Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products

Supercritical Fractionation of Rosemary Extracts to Improve the Antioxidant Activity

Contact Info

Product

Resources

About