A key amino acid determining G3m(b) allotypic markers

Ito, Shigenori; Suzuki, Koichi; Miyazaki, Tokiko; Matsumoto, Hideo

doi:10.1007/bf01876582

Cited by 2 publications

(25 citation statements)

References 26 publications

(24 reference statements)

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“…The G3m 21,28 haplotype is also highly variable at the DNA level, as four distinct sequences are found among 10 G3m 21,28 genes, and the three G3m 21,28 sequences observed in the Selkups are different from each other (S+F, S1, S2). The sequences associated to haplotype G3m 10,11,13,15,16 vary among populations, as that found in Lebanese (L2, n=3) differ from that found in Selkups (S3, n=6) by one substitution. Finally, G3m 5,6,10,11,14 (M8, n=2), G3m 10,11,13,15 (M9, n=4), and G3m 5,6,11,24 (M+T, n=9) sequences are monomorphic.…”

Section: Dna Sequence Variability Of Ighg3 Genes P Dard Et Al 766mentioning

confidence: 83%

“…The sequences associated to haplotype G3m 10,11,13,15,16 vary among populations, as that found in Lebanese (L2, n=3) differ from that found in Selkups (S3, n=6) by one substitution. Finally, G3m 5,6,10,11,14 (M8, n=2), G3m 10,11,13,15 (M9, n=4), and G3m 5,6,11,24 (M+T, n=9) sequences are monomorphic. These haplotypes were only sequenced in the Mandenka except one G3m 5,6,11,24 in a Tunisian, which is identical to the Mandenka sequence (M+T).…”

Section: Dna Sequence Variability Of Ighg3 Genes P Dard Et Al 766mentioning

confidence: 83%

“…Neighbor-Joining (NJ), 30 UPGMA, 31 and Maximum 10,11,13,15 Mandenka Senegal (West Africa) 4 G3m 5,6,11,24 Mandenka Senegal (West Africa) 8…”

Section: Sequence Alignment and Phylogenetic Analysesmentioning

confidence: 99%

“…Sequence clusters are shown to be G3m haplotype-specific, corroborating the Gm molecular model deduced from serology, and showing that populations differentiation is much more recent than G3m haplotypes differentiation. The widely distributed G3m 5,10,11,13,14 haplotype is likely to be ancestral to the other G3m haplotypes presently found at high frequencies in different continental areas. European Journal of Human Genetics (2001) 9, 765 ± 772.…”

mentioning

confidence: 99%

“…1 It is defined serologically by 18 allotypes specific to g1 (G1m 1, 2, 3, 17), g2 (G2m 23) and g3 (G3m 5,6,10,11,13,14,15,16,21,24,26,27,28). The IGHG1, IGHG2 and IGHG3 genes are physically linked on chromosome 14q32.3.…”

Section: Introductionmentioning

confidence: 99%

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DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations

et al. 2001

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The present study investigates the molecular basis of the G3m polymorphism expressed by the heavy constant domains of human immunoglobulins gamma 3 chains. By using a new protocol allowing the specific cloning of IGHG3 genes, a total of 51 full-length IGHG3 genomic sequences (about 2 kb) isolated from African, Siberian, West Asian and European population samples were sequenced. IGHG3 sequences were assigned precise G3m haplotypes on the basis of specific associations between G3m allotypes and IGHG3 RFLPs. Specific DNA substitutions involved in the expression of G3m(5), G3m(6), G3m(15), G3m(16), G3m(21), G3m(24) and G3m(28) allotypes were then deduced, elucidating almost completely the determination of the G3m polymorphism at the DNA level. The molecular evolution of G3m haplotypes was investigated by a maximum likelihood phylogeny of IGHG3 sequences. Sequence clusters are shown to be G3m haplotype-specific, corroborating the Gm molecular model deduced from serology, and showing that populations differentiation is much more recent than G3m haplotypes differentiation. The widely distributed G3m 5,10,11,13,14 haplotype is likely to be ancestral to the other G3m haplotypes presently found at high frequencies in different continental areas. European Journal of Human Genetics (2001) 9, 765 ± 772.

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Section: Dna Sequence Variability Of Ighg3 Genes P Dard Et Al 766mentioning

confidence: 83%

Section: Dna Sequence Variability Of Ighg3 Genes P Dard Et Al 766mentioning

confidence: 83%

“…Neighbor-Joining (NJ), 30 UPGMA, 31 and Maximum 10,11,13,15 Mandenka Senegal (West Africa) 4 G3m 5,6,11,24 Mandenka Senegal (West Africa) 8…”

Section: Sequence Alignment and Phylogenetic Analysesmentioning

confidence: 99%

mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations

et al. 2001

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DNA sequences specific for Caucasian G3m(b) and (g) allotypes: allotyping at the genomic level

et al. 1994

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Assignment of the G3m(g) and (b) correlative amino acid residues was performed at the genomic level by direct sequencing of DNA from nine Caucasian individuals. Two oligonucleotide primers were used for subclass-specific enzymatic amplification of a DNA segment comprising a major portion of the second and third constant region domains (CH2 and CH3) of the human IgG3 heavy chain gene. Comparison of the sequences of amplified DNA from individuals serologically typed as homozygous for G3m(b) or G3m(g) or as heterozygous, G3m(b,g), revealed differences in the codons for the amino acid residues 291, 296, and 384. Proline, phenylalanine, and serine at these positions corresponded to G3m(b), and leucine, tyrosine, and asparagine to G3m(g). Heterozygotic individuals, typed G3m(b,g), displayed both the G3m(b) and G3m(g) codons at these three positions. The polymorphism at each of these three codons could be identified either as the appearance, or the loss, of recognition sites for the two restriction endonucleases, Nsp BII and Rsa I. This allowed the development of a polymerase chain reaction (PCR)-based assay permitting the distinction of G3mb and G3mg alleles by analyzing the electrophoretical mobility of the DNA fragments generated by digestion of the PCR-products with Nsp BII and Rsa I.

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A key amino acid determining G3m(b) allotypic markers

Cited by 2 publications

References 26 publications

DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations

DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations

DNA sequences specific for Caucasian G3m(b) and (g) allotypes: allotyping at the genomic level

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