A Janus-paper PDMS platform for air–liquid interface cell culture applications

Rahimi, Rahim; Ochoa, Manuel; Donaldson, Amy R.; Parupudi, Tejasvi; Dokmeci, Mehmet R.; Khademhosseini, Ali; Ghaemmaghami, Amir M.; Ziaie, Babak

doi:10.1088/0960-1317/25/5/055015

Cited by 16 publications

(15 citation statements)

References 25 publications

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“…GelMA has also found application niches in fundamental research. For example, it has been used to devise chips for the capture, culture, and study of a single neuron (Figure 6B) [40] and to build 3D microfluidic systems (or components of them) for cell culture applications [38, 107]. Recently, Topkaya et al .…”

Section: Biomedical Applications Of Gelma Hydrogelsmentioning

confidence: 99%

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

et al. 2015

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Gelatin methacryloyl (GelMA) hydrogels have been widely used for various biomedical applications due to their suitable biological properties and tunable physical characteristics. Three dimensional (3D) GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM) due to the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs, which allow cells to proliferate and spread in GelMA-based scaffolds. GelMA is also versatile from a processing perspective. It crosslinks when exposed to light irradiation to form hydrogels with tunable mechanical properties which mimic the native ECM. It can also be microfabricated using different methodologies including micromolding, photomasking, bioprinting, self-assembly, and microfluidic techniques to generate constructs with controlled architectures. Hybrid hydrogel systems can also be formed by mixing GelMA with nanoparticles such as carbon nanotubes and graphene oxide, and other polymers to form networks with desired combined properties and characteristics for specific biological applications. Recent research has demonstrated the proficiency of GelMA-based hydrogels in a wide range of applications including engineering of bone, cartilage, cardiac, and vascular tissues, among others. Other applications of GelMA hydrogels, besides tissue engineering, include fundamental single-single cell research, cell signaling, drug and gene delivery, and bio-sensing.

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Section: Biomedical Applications Of Gelma Hydrogelsmentioning

confidence: 99%

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

et al. 2015

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“…At present, various methods including plasma treatments, corona discharge, ultraviolet irradiation, chemical vapor deposition, and coating or grafting hydrophilic polymers onto PDMS surface have been proposed to improve the surface hydrophilicity of PDMS. Although these methods significantly improve the wettability of the PDMS surface, some modification methods, such as plasma treatment, cannot make the surface hydrophilicity last long because of the surface enrichment of the siloxane.…”

Section: Inroductionmentioning

confidence: 99%

Reverse hydrophobic PDMS surface to hydrophilic by 1‐step hydrolysis reaction

Zhang

Kalulu

Wang

et al. 2018

Polymers for Advanced Techs

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Hydrophilic modification on the surface of polymer polydimethylsiloxane (PDMS) material is a key step for its application in biomaterial, bioengineering, and so on. In this article, a novel and effective method was proposed to reverse hydrophobic surface to hydrophilic by 1‐step hydrolysis of Si―O bond to produce hydrophilic hydroxyl group. The hydrophilizing reagent 2‐(trimethylsiloxy) ethyl methacrylate (TMSEMA) was used during the copolymerization of polydimethylsiloxane prepolymer (DMS U21). The prepared PDMS film was subjected to 1‐step surface hydrophilic reversal treatment using KOH solution to produce hydroxyl groups on the surface. The contact angle, attenuated total reflection Fourier transform infrared spectra, and equilibrium water content (EWC) measurements were conducted on PDMS films. The results showed that TMSEMA content had no obvious impact on the contact angle and EWC value of untreated PDMS. After reversal treatment, the contact angle decreased from 94° to 15°, and the EWC value increases to 10% when the TMSEMA content was 15 wt%. The spectrum proved that the reverse reaction produced hydroxyl and carboxylate on the surface. The hydrophilic stability, surface morphology, and protein adsorption properties of PDMS film were also investigated. This study can provide new ideas and further reference for improving the hydrophilicity of PDMS surface.

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“…Paper was also used as a platform for air–liquid interface cell cultures . The air–liquid interface cell culture systems provide a more biomimetic microenvironment for cells compared to trans ‐well assays or other conventional in vitro methods .…”

Section: Paper‐based Cell Culture Platforms To Study Mammalian Cellsmentioning

confidence: 99%

Section: Paper‐based Cell Culture Platforms To Study Mammalian Cellsmentioning

confidence: 99%

Engineered Paper‐Based Cell Culture Platforms

Lantigua

Kelly

Ünal

et al. 2017

Adv Healthcare Materials

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Paper is used in various applications in biomedical research including diagnostics, separations, and cell cultures. Paper can be conveniently engineered due to its tunable and flexible nature, and is amenable to high-throughput sample preparation and analysis. Paper-based platforms are used to culture primary cells, tumor cells, patient biopsies, stem cells, fibroblasts, osteoblasts, immune cells, bacteria, fungi, and plant cells. These platforms are compatible with standard analytical assays that are typically used to monitor cell behavior. Due to its thickness and porous nature, there are no mass transport limitations to/from the cells in paper scaffolds. It is possible to pattern paper in different scales (micrometer to centimeter), generate modular configurations in 3D, fabricate multicellular and compartmentalized tissue mimetics for clinical applications, and recover cells from the scaffolds for further analysis. 3D paper constructs can provide physiologically relevant tissue models for personalized medicine. Layer-by layer strategies to assemble tissue-like structures from low-cost and biocompatible paper-based materials offer unique opportunities that include understanding fundamental biology, developing disease models, and assembling different tissues for organ-on-paper applications. Paper-based platforms can also be used for origami-inspired tissue engineering. This work provides an overview of recent progress in engineered paper-based biomaterials and platforms to culture and analyze cells.

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A Janus-paper PDMS platform for air–liquid interface cell culture applications

Cited by 16 publications

References 25 publications

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

Reverse hydrophobic PDMS surface to hydrophilic by 1‐step hydrolysis reaction

Engineered Paper‐Based Cell Culture Platforms

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