A trnH-psbA barcode genotyping assay for the detection of common apricot (Prunus armeniaca L.) adulteration in almond (Prunus dulcis Mill.)

Uncu, Ayşe Özgür

doi:10.1080/19476337.2020.1727961

Cited by 6 publications

(6 citation statements)

References 48 publications

(69 reference statements)

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“…DNA barcoding efficiency has been widely demonstrated in discriminating spices species such as nutmeg [ 49 ]. Recently, the analysis of trnH-psbA spacer and ITS2 sequence revealed them to be effective in the authentication of ginseng products [ 95 ] and the identification of adulterants in coffee and almond [ 50 , 96 ].…”

Section: Molecular Approaches To Agri-food Analysismentioning

confidence: 99%

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Fanelli

Mascio

Miazzi

et al. 2021

Foods

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In the last decades, the demand for molecular tools for authenticating and tracing agri-food products has significantly increased. Food safety and quality have gained an increased interest for consumers, producers, and retailers, therefore, the availability of analytical methods for the determination of food authenticity and the detection of major adulterations takes on a fundamental role. Among the different molecular approaches, some techniques such as the molecular markers-based methods are well established, while some innovative approaches such as isothermal amplification-based methods and DNA metabarcoding have only recently found application in the agri-food sector. In this review, we provide an overview of the most widely used molecular techniques for fresh and processed agri-food authentication and traceability, showing their recent advances and applications and discussing their main advantages and limitations. The application of these techniques to agri-food traceability and authentication can contribute a great deal to the reassurance of consumers in terms of transparency and food safety and may allow producers and retailers to adequately promote their products.

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Section: Molecular Approaches To Agri-food Analysismentioning

confidence: 99%

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Fanelli

Mascio

Miazzi

et al. 2021

Foods

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“…These regions were latter used by many researchers to investigate the systematics and phylogentic relationships of Prunus species [18,19,25,26]. Meanwhile, Uncu [27] used trnH-psbA region sucessefully to detect the fraud of apricot kernels to the almond valuable oil.…”

Section: Concatenated Sequences-based Phylogenetic Treementioning

confidence: 99%

DNA Barcoding of Prunus Species Collection Conserved in the National Gene Bank of Egypt

et al. 2022

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Two intergenic spacers cpDNA barcoding regions were used to assess the genetic diversity and phylogenetic structure of a collection of 25 Prunus accessions. The trnH-psbA and trnL-trnF intergenic spacers were able to distinguish and identify only four Prunus species. The average aligned length was 316–352 bp and 701–756 bp for trnH-psbA and trnL-trnF, respectively. The overall evolutionary divergence was higher in trnH-psbA than trnL-trnF. The transition/transversion bias (R) recorded as 0.59 in trnL-trnF and 0.89 in trnH-psbA. The number of invariable sites, nucleotide diversity (Pi), and the average number of nucleotide differences (k) was higher in the trnH-psbA region. The trnL-trnF records was above the other region in the number of variable sites, number of singleton variable sites, and the parsimony informative sites. Phylogenetic relationships among the 25 accessions of Prunus species were investigated. Most of the different Prunus species clustered in a homogenized distribution in both regions, except for the plum (P. domestica) accession (African Rose) was assigned with the peach (P. persica) accessions. The two intergenic cpDNA trnH-psbA and trnL-trnF were able to distinguish and identify the four Prunus species accessions.

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“…CE methods have also been reported in the literature for the detection of DNA markers of food adulteration (such as the addition of green pea kernels to pistachio nuts [79], the use of apricot kernels as almond substitutes [80], or the use of Asian black truffles as black Perigord truffles [81]), or for food authentication [82]). In the case of pistachio adulteration, a barcode DNA genotyping approach using the trnL (UAA)‐trnF (GAA) intergenic spacer in the plastid genome was used to identify green pea adulteration in pistachio nuts [79].…”

Section: Dnasmentioning

confidence: 99%

“…The PCR‐CE method allowed the identification of different in‐house admixtures, from 5% to 30% of green pea. A similar approach was used for the identification of apricot kernels in almond products, but in this case, the trnH‐psbA intergenic spacer was selected for the discrimination of almond and apricot [80]. Barcode DNA was also used for the detection of mandarin and grapefruit juice adulteration in orange juice [83].…”

Section: Dnasmentioning

confidence: 99%

“…By using a variant of competitive PCR for fragment amplification, relative quantification of Trait versus Non-Trait amplicons was performed, and the CE method allowed the quantification of 5% increments across the 0-100% range with a mean absolute error of 1.9%. CE methods have also been reported in the literature for the detection of DNA markers of food adulteration (such as the addition of green pea kernels to pistachio nuts [79], the use of apricot kernels as almond substitutes [80], or the use of Asian black truffles as black Perigord truffles [81]), or for food authentication [82]). In the case of pistachio adulteration, a barcode DNA genotyping approach using the trnL (UAA)-trnF (GAA) intergenic spacer in the plastid genome was used to identify green pea adulteration in pistachio nuts [79].…”

Section: Dnasmentioning

confidence: 99%

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Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period February 2019–February 2021

et al. 2021

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This work presents a revision of the main applications of capillary electromigration methods in food analysis and Foodomics. Articles that were published during the period February 2019‐February 2021 are included. The work shows the multiple CE methods that have been developed and applied to analyze different types of molecules in foods. Namely, CE methods have been applied to analyze amino acids, biogenic amines, carbohydrates, chiral compounds, contaminants, DNAs, food additives, heterocyclic amines, lipids, secondary metabolites, peptides, pesticides, phenols, pigments, polyphenols, proteins, residues, toxins, vitamins, small organic and inorganic compounds, as well as other minor compounds. The last results on the use of CE for monitoring food interactions and food processing, including recent microchips developments and new applications of CE in Foodomics, are discussed too. The new procedures of CE to investigate food quality and safety, nutritional value, storage and bioactivity are also included in the present review work.

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A trnH-psbA barcode genotyping assay for the detection of common apricot (Prunus armeniaca L.) adulteration in almond (Prunus dulcis Mill.)

Abstract: To cite this article: Ayse Ozgur Uncu (2020) A trnH-psbA barcode genotyping assay for the detection of common apricot (Prunusarmeniaca L.

Cited by 6 publications

References 48 publications

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

DNA Barcoding of Prunus Species Collection Conserved in the National Gene Bank of Egypt

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period February 2019–February 2021

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