A <i>p</i>‐Menth‐1‐ene‐4,7‐diol (EC‐1) from <i>Eucalyptus camaldulensis</i> Dhnh. Triggers Apoptosis and Cell Cycle Changes in Ehrlich Ascites Carcinoma Cells

Islam, Farhadul; Khanam, Jahan Ara; Khatun, Mahbuba; Zuberi, Natasha; Khatun, Laboni; Kabir, Syed Rashel; Reza, Abu; Mm, Ali; Rabbi, M. Ahasanur; Gopalan, Vinod

doi:10.1002/ptr.5288

Cited by 31 publications

(18 citation statements)

References 33 publications

(37 reference statements)

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“…Morphological observation of EAC cells in both treated (0.3 mg/kg/day) and control groups were carried out using an optical and fluorescence microscope (Olympus IX71, Seoul, Korea) . In short, EAC cells were harvested from the experimental mice and stained with 0.1 μg/ml of Hoechst 33342 at 37°C for 20 min in dark.…”

Section: Methodsmentioning

confidence: 99%

Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes

Zahan

Rahi

Sheikh

et al. 2018

Applied Organom Chemis

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Nickel is a fundamental element for healthy life for human and higher animals. For biological importance, its complexation with bioactive ligand is worth to be studied with the aim to understand its function. Using mouse peritoneal cancer model, MTT colorimetric assay and anticancer activity analysis, we examined the role of nickel(ll) complex in growth inhibition of cancer cells. A novel nickel(ll) complex was synthesized and characterized using physico‐chemical and spectroscopic techniques. The study indicated that both the ligand and complex were capable of inhibiting Ehrlich Ascites Carcinoma (EAC) cells growth by 28.21% and 44.52%, respectively, when administered 0.3 mg/kg/day body weight intraperitoneally for five consecutive days in Swiss Webstar mice. Determination the LD50 of the complex (55 mg/kg) allowed adjusting the dose as 2.75 mg/kg and upon administration, inhibition increased to 69.36%. The ligand and complex have shown an inhibitory effect in the range of 4.86%–67.3% and 6.1%‐ 89.37%, respectively, against EAC cells (concentration range of 31.25–500 μg/ml) in RPMI‐1640 medium as determined by MTT colorimetric assay. Apoptotic cell morphological alteration was determined through optical and fluorescence microscopy. Up regulation of P53, Bax, Cas‐8, Cas‐3 and Fas and down regulation of NF‐kB and Bcl‐2 gene expression were observed in the cells treated with the nickel(ll) complex for five consecutive days. In conclusion, the newly synthesized nickel(ll) complex has shown anti‐proliferative activity and can further be optimized to be used as a lead molecule for anticancer drug.

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Section: Methodsmentioning

confidence: 99%

Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes

Zahan

Rahi

Sheikh

et al. 2018

Applied Organom Chemis

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“…MTT assay showed dose-dependent anticancer activity of EC-1 against EAC cell. Cell cycle analysis revealed that EC-1 treatment caused suppression of EAC cells at S phase [132]. The cytotoxic effect of the crude methanolic extracts of Eucalyptus camaldulensis was investigated against L20B (a genetically engineered mouse cell line) and human rhabdomyo sarcoma (RD) cells showed that the extract of Eucalyptus camaldulensis possessed moderate cytotoxicity [133].…”

Section: Cytotoxic Effectmentioning

confidence: 99%

The pharmacological and therapeutic importance of Eucalyptus species grown in Iraq

Al-Snafi¹

2017

IOSRPHR

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Abstract:-Eucalyptus species grown in Iraq were included Eucalyptus bicolor (Syn: Eucalyptus largiflorens), Eucalyptus griffithsii, Eucalyptus camaldulensis (Syn: Eucalyptus rostrata) Eucalyptus incrassate, Eucalyptus torquata and Eucalyptus microtheca (Syn: Eucalyptus coolabahs). Eucalypts contained volatile oils which occurred in many parts of the plant, depending on the species, but in the leaves that oils were most plentiful. The main constituent of the volatile oil derived from fresh leaves of Eucalyptus species was 1,8-cineole. The reported content of 1,8-cineole varies for 54-95%. The most common constituents co-occurring with 1,8-cineole were limonene, α-terpineol, monoterpenes, sesquiterpenes, globulol and α , β and ϒ-eudesmol, and aromatic constituents. The pharmacological studies revealed that Eucalypts possessed gastrointestinal, antiinflammatory, analgesic, antidiabetic, antioxidant, anticancer, antimicrobial, antiparasitic, insecticidal, repellent, oral and dental, dermatological, nasal and many other effects. The current review highlights the chemical constituents and pharmacological and therapeutic activities of Eucalyptus species grown in Iraq.

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“…FAM134B knockdown and control/non‐transfected wild type cells were fixed with cold 70% ethanol for one hour as previously described . After washing with cold PBS, 5 µl of RNase A (10 mg/ml) was added to the cells and incubated for one hour at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Stage dependent expression and tumor suppressive function ofFAM134B(JK1) in colon cancer

et al. 2016

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The aims of the present study are to investigate sub-cellular location, differential expression in different cancer stages and functional role of FAM134B in colon cancer development. FAM134B expression was studied and quantified at protein and mRNA levels in cell lines using immunocytochemistry, Western blot and real-time PCR. In vitro functional assays and an in vivo xenotransplantation mouse models were used to investigate the molecular role of FAM134B in cancer cell biology in response to FAM134B silencing with shRNA lentiviral particles. FAM134B protein was noted in both cytoplasm and nuclei of cancer cells. In cancer cells derived from stage IV colon cancer, FAM134B expression was remarkably reduced when compared to non-cancer colon cells and cancer cells derived from stage II colon cancer. FAM134B knockdown significantly (P < 0.05) increased the proliferation of colon cancer cells following lentiviral transfection. Furthermore, FAM134B suppression significantly increased (34-52%; P < 0.05) the clonogenic capacity, wound healing potential of and increases the proportion of cells performing DNA synthesis (P < 0.01). Xenotransplantation model showed that larger and higher-grade tumors were formed in mice receiving FAM134B knockdown cells. To conclude, expression analysis, in vitro and in vivo indicated that FAM134B acts as a cancer suppressor gene in colon cancer. © 2016 Wiley Periodicals, Inc.

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A p‐Menth‐1‐ene‐4,7‐diol (EC‐1) from Eucalyptus camaldulensis Dhnh. Triggers Apoptosis and Cell Cycle Changes in Ehrlich Ascites Carcinoma Cells

Cited by 31 publications

References 33 publications

Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes

Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes

The pharmacological and therapeutic importance of Eucalyptus species grown in Iraq

Stage dependent expression and tumor suppressive function ofFAM134B(JK1) in colon cancer

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