A Hyperdry Amniotic Membrane Patch Using a Tissue Adhesive for Corneal Perforations and Bleb Leaks

Kitagawa, Kiyotaka; Yanagisawa, Shuichiro; Watanabe, Kazuhiko; Yunoki, Tatsuya; Hayashi, Atsushi; Okabe, Motonori; Nikaido, Toshio

doi:10.1016/j.ajo.2009.03.030

Cited by 126 publications

(44 citation statements)

References 31 publications

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“…As previously reported, 11 we successfully treated patients with corneal perforations up to 2.5 mm in diameter and bleb leaks up to 1 mm in diameter using HD-AM in combination with tissue adhesive. The healing time for repair of those conditions was within 21 days.…”

Section: Discussionsupporting

confidence: 53%

“…We previously reported that HD-AM histologically recovered a layer structure similar to that of fresh AM when the HD-AM absorbed water. 11 We have also confi rmed that the collagen structures in the HD-AM stroma do not melt, and that collagen IV and laminin can be detected immunohistochemically (unpublished data). Similar to the HD-AM, HDCL-AM in the dry state is easy to handle and cut to the desired size and shape with scissors just before application.…”

Section: Discussionmentioning

confidence: 87%

“…We recently reported on the effi cacy of the HD-AM patch in combination with use of a tissue adhesive in treating corneal perforations or bleb leaks. 11 In the present study, we developed a new HDCL-AM by combining the methods of Spoerl et al 9 and Toda et al 10 to improve both the durability and the convenience of AM in ophthalmic clinical applications. We applied this HDCL-AM patching to cases of corneal perforations and also evaluated the resistance of HDCL-AM to collagenase digestion by performing in vitro digestion testing.…”

Section: Introductionmentioning

confidence: 98%

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Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations

et al. 2011

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 98%

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Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations

et al. 2011

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“…Written informed consent from the donor (participant age range [25][26][27][28][29][30][31][32][33][34][35][36][37] or the next of kin was obtained for sample use in this research project and prepared according to previously published methodology [45]. Patients with a history of antenatal problems e.g.…”

Section: Tissue Procurement and Preparationmentioning

confidence: 99%

“…A number of studies have reported extensive depletion of soluble factors, presumed to be beneficial, from cryopreserved AM [26][27][28]. While frozen preparations of AM account for the majority of procedures, dried preparations have gained popularity as substrates for epithelial growth during ocular surface reconstruction [29,30], to treat corneal perforations and leaks [31] and pterygium [32]. Moreover, as dried preparations can be kept at room temperature, they eliminate the need for a cold chain and are therefore suitable for use in developing countries and in military environments.…”

Section: Introductionmentioning

confidence: 99%

Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

Allen¹,

Clare²,

Stewart³

et al. 2013

Acta Ophthalmologica

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Purpose: Dried amniotic membrane (AM) can be a useful therapeutic adjunct in ophthalmic surgery and possesses logistical advantages over cryopreserved AM. Differences in preservation techniques can significantly influence the biochemical composition and physical properties of AM, potentially affecting clinical efficacy. This study was established to investigate the biochemical and structural effects of drying AM in the absence and presence of saccharide lyoprotectants and its biocompatibility compared to cryopreserved material.Methods: AM was cryopreserved or dried with and without pre-treatment with trehalose or raffinose and the antioxidant epigallocatechin (EGCG). Structural and visual comparisons were assessed using electron microscopy. Localisation, expression and release of AM biological factors were determined using immunoassays and immunofluorescence. The biocompatibility of the AM preparations co-cultured with corneal epithelial cell (CEC) or keratocyte monolayers were assessed using cell proliferation, cytotoxicity, apoptosis and migration assays.Results: Drying devitalised AM epithelium, but less than cryopreservation and cellular damage was reduced in dried AM pre-treated with trehalose or raffinose. Dried AM alone, and with trehalose or raffinose showed greater factor retention efficiencies and bioavailability compared to cryopreserved AM and demonstrated a more sustained biochemical factor time release in vitro. Cellular health assays showed that dried AM with trehalose or raffinose are compatible and superior substrates compared to cryopreserved AM for primary CEC expansion, with increased proliferation and reduced LDH and caspase-3 levels. This concept was supported by improved wound healing in an immortalised human CEC line (hiCEC) cocultured with dried and trehalose or raffinose membranes, compared to cryopreserved and fresh AM.Conclusions: Our modified preservation process and our resultant optimised dried AM has enhanced structural properties and biochemical stability and is a superior substrate to conventional cryopreserved AM. In addition this product is stable and easily transportable allowing it to be globally wide reaching for use in clinical and military sectors.

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Hyperdry human amniotic membrane is useful material for tissue engineering: Physical, morphological properties, and safety as the new biological material

Okabe

Kitagawa

Yoshida

et al. 2013

J Biomedical Materials Res

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Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by γ-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the physical and histological properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dried methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. HD-AM has kept the morphological structure of epithelium and connective tissues. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium.

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A Hyperdry Amniotic Membrane Patch Using a Tissue Adhesive for Corneal Perforations and Bleb Leaks

Cited by 126 publications

References 31 publications

Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations

Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations

Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

Hyperdry human amniotic membrane is useful material for tissue engineering: Physical, morphological properties, and safety as the new biological material

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