A hydroxyl PEG version of PEGylated liposomes and its impact on anti-PEG IgM induction and on the accelerated clearance of PEGylated liposomes

Shimizu, Taro; Lila, Amr S. Abu; Fujita, Risako; Awata, Mizuki; Kawanishi, Munehira; Hashimoto, Yuichi; Okuhira, Keiichiro; Ishima, Yu; Ishida, Tatsuhiro

doi:10.1016/j.ejpb.2018.02.019

Cited by 65 publications

(65 citation statements)

References 41 publications

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“…Thus, to overcome this disadvantage, surface passivation scheme has been used to increase the fluorescence properties of CDs and also to make them appropriate for biological applications [31]. Among the various organic molecules served as a CDs surface passivation, polyethylene glycol (PEG) has been more concerned, due to excellent biocompatibility, non-toxicity and hydrophilic properties [32][33][34][35][36]. Conjugating PEG onto the surface of CDs (PEGylation) would be an ideal approach to improving the biocompatibility and avoid recognition and clearance of the CDs by the reticuloendothelial system (RES) which increases the circulation halftime of CDs [37,38].…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted and one-step synthesis of PEG passivated fluorescent carbon dots from gelatin as an efficient nanocarrier for methotrexate delivery

Arsalani

Nezhad‐Mokhtari

Jabbari

2019

Artificial Cells, Nanomedicine, and Biotechnology

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A green and simple process for preparing the polyethylene glycol passivated fluorescent carbon dots (CDs-PEG) have been studied by a microwave pyrolysis method, using gelatin and PEG as starting materials. This method is very effective for development of carbon-based quantum dots from gelatin with high quantum yield (QY). The synthesized CDs-PEG were found to emit blue photoluminescence (PL) with a maximum QY of 34%. At the following research, we investigated the effect of the presence of PEG on PL intensity, and the result showed that CDs-PEG becomes stronger PL properties than pure CDs from gelatin. The synthesized CDs-PEG were characterized by FTIR, TEM, UV-vis, PL, zeta potential and XRD analyses. The anticancer performance of developed CDs-PEG was evaluated by in vitro tests such as MTT assay and fluorescence microscopy analyses. The examination of CDs-PEG as an anticancer drug nanocarrier for methotrexate (MTX) illustrated a better antitumor efficacy than free MTX due to its enhanced nuclear delivery in vitro, which resulting in highly effective tumour growth inhibition and improving targeted cancer therapy in clinical medicine.

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Section: Introductionmentioning

confidence: 99%

Microwave-assisted and one-step synthesis of PEG passivated fluorescent carbon dots from gelatin as an efficient nanocarrier for methotrexate delivery

Arsalani

Nezhad‐Mokhtari

Jabbari

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Thus, PEG forms a water shell around therapeutic drug molecules and makes a nonspecific steric hindrance barrier preventing their binding to serum proteins (opsonins) [26]. Moreover, PEG has the ability to prevent cellular as well as humoral immunogenicity [27]. PEGylation efficacy is highly related to both the length and the coating density of PEG on liposomes surfaces, as very short PEG (ex: PEG1K or less) lack the ability to prevent the interaction of PEGylated liposomes with serum proteins thereby cannot effectively prolong the circulation time.…”

Section: Pegylation Of Liposomesmentioning

confidence: 99%

“…PEG is commonly used for surface modification of nanocarrier system to sustain the biological half-life of drugs and prevent their clearance by RES because of its poor immunogenic and antigenic properties [50]. A number of studies showed that specific anti-PEG antibodies could be formed toward PEGylated formulations [27,51,52]. It was assumed that eliciting immunogenic response against PEG can take place in humans.…”

Section: Immunogenicity Of Pegmentioning

confidence: 99%

Liposomes and PEGylated liposomes as drug delivery system

Mohamed

Alaaeldin

Hussein

et al. 2020

Journal of advanced Biomedical and Pharmaceutical Sciences

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Generally, it has been thought that PEG-conjugated nanocarriers are non-immunogenic. However, many reports have revealed that unexpected immune responses occur with such PEG-conjugated nanocarriers. The Most important one is the rapid clearance of PEGylated nanocarriers upon repeated administration which is called accelerated blood clearance phenomenon involving the production of antibodies against nanocarrier components, which reduces the safety and effectiveness of the encapsulated therapeutic agent. Such immunogenicity of PEGylated nanocarriers is a potential concern in the evaluation and clinic use of PEGylated therapeutics. Accordingly, screening of the immunogenicity of nanocarriers-based therapeutics is a prerequisite before their adoption into clinical settings to disclose any possible interactions with immune system. This review gives an overview of PEGylated liposomes, immunogenicity of PEG, explanation of accelerated blood clearance (ABC) phenomenon, its mechanism, various factors affecting it and side effects of PEGylated liposomes.

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“…In order to adjust its physicochemical properties and increase its hydrophilicity, PLLA can be copolymerized with hydrophilic polymers so as to create amphiphilic copolymers. At present, many such copolymers based on PLLA have been developed, and their in vivo and in vitro properties have been studied . In fact, PLLA is commonly used as a hydrophobic segment to synthesize amphiphilic block copolymers with hydrophilic segments such as PNIPAAm and PEG.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, PLLA is commonly used as a hydrophobic segment to synthesize amphiphilic block copolymers with hydrophilic segments such as PNIPAAm and PEG. Recently, various PLLA‐PEG and PLLA‐PNIPAAm block copolymers have been reported.…”

Section: Introductionmentioning

confidence: 99%

PLLA‐Grafted Gelatin Amphiphilic Copolymer and Its Self‐Assembled Nano Carrier for Anticancer Drug Delivery

Fan

et al. 2019

Macro Chemistry & Physics

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A series of poly(l‐lactide)‐grafted gelatin (Gel‐g‐PLLA) copolymers are synthesized by coupling the amino groups of gelatin with different molar masses and the carboxyl endgroup of poly(l‐lactide) in the presence of 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide hydrochloride and N‐hydroxysuccinimide. Fourier transform infrared and nuclear magnetic resonance (1H NMR) data confirm the successful bonding between gelatin and PLLA. Self‐assembled micelles of copolymers are prepared by using the direct dissolution method. The size and micellar morphology are determined from dynamic light scattering as well as transmission electron microscopy measurements. Meanwhile, in vitro drug release reveals that Gel‐g‐PLLA micelles show excellent sustained‐release properties when used as the carrier of the anticancer drug paclitaxel. A burst release of about 70% is observed in the first 24 h. All these features, together with the outstanding biocompatibility, make Gel‐g‐PLLA micelles a promising drug carrier for cancer therapy.

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A hydroxyl PEG version of PEGylated liposomes and its impact on anti-PEG IgM induction and on the accelerated clearance of PEGylated liposomes

Cited by 65 publications

References 41 publications

Microwave-assisted and one-step synthesis of PEG passivated fluorescent carbon dots from gelatin as an efficient nanocarrier for methotrexate delivery

Microwave-assisted and one-step synthesis of PEG passivated fluorescent carbon dots from gelatin as an efficient nanocarrier for methotrexate delivery

Liposomes and PEGylated liposomes as drug delivery system

PLLA‐Grafted Gelatin Amphiphilic Copolymer and Its Self‐Assembled Nano Carrier for Anticancer Drug Delivery

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