We report on a simple strategy for the fabrication of gold nanoparticles (AuNPs) on an indium tin oxide substrate using a modified ion implantation method. The morphology, structure and electrochemical features of AuNPs were characterized by atomic force microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode has a large electrochemically active surface and enables strong loading with cytochrome c (Cyt c) proteins. It undergoes enhanced electron transfer at uncompromised electrochemical activity, and also displays good stability and repeatability. The immobilized Cyt c exhibits good electrocatalytic activity towards hydrogen peroxide (H 2 O 2 ), with a linear relationship between the catalytic current during differential pulse voltammetry and the concentration of H 2 O 2 in the 0.05 μM to 0.2 μM range. The detection limit (S/N 0 3) is 0.01 μM.