A Novel Hydrogen Peroxide Biosensor Based on Adsorption of Horseradish Peroxidase onto a Nanobiomaterial Composite Modified Glassy Carbon Electrode

Abstract

In this work, a novel hydrogen peroxide biosensor derived from maize tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The morphology and structure of the products were characterized by SEM, FTIR and UV‐visible spectroscopy. The electrochemical and electrocatalytic performance of the HRP/MT‐MWCNT/GCE was studied using voltammetric and amperometric methods. The amperometric response of the biosensor varied linearly with concentration of H2O2 from 9 µM to 1 mM with detection limit of 4.0 µM (S/N=3). Furthermore, the biosensor exhibited good reproducibility and stability.

Improved electro-oxidation of triclosan at nano-zinc oxide-multiwalled carbon nanotube modified glassy carbon electrode

“…It is well known that CNTs are suitable materials for modification of electrodes and support in sensor applications because of good porosity, large aspect ratio, high electrical conductivity and high chemical stability [27,28]. Consequently, CNTs can be used to immobilize nZnO onto electrodes.…”

Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions

“…The biosensor was prepared following the steps described in our previous work [8]. Briefly, prior to modification, the GC electrode (ɸ = 4 mm) was polished to a mirror finish by use of the BASi polishing kit with 1.0, 0.3 and 0.05 μm diamond slurry in sequence, rinsed thoroughly with ultra-pure water, then ultrasonically rinsed with ethanol and ultra-pure water for 10 min in sequence, in order to remove any adsorbed substances on the electrode surface.…”

“…They possess advantages such as minimum sample pretreatment, low cost and less time of analysis, and sufficient sensitivity and selectivity [6][7][8]. In inhibition-based enzyme biosensors, the concentration of heavy metal ions in an assayed sample is determined through either direct or indirect inhibition methods with lower detection M. MOYO 2 limits.…”

A simple and sensitive hydrogen peroxide detection with horseradish peroxidase immobilized on pyrene modified acid‐treated single‐walled carbon nanotubes

“…used chitosan‐wrapped single‐walled carbon nanotubes (SWCNTs) for direct bioelectrochemical sensing of H 2 O 2 using the HRP enzyme without any mediator . Maize tassel and multiwalled carbon nanotube composite materials were also used as an adsorption matrix for HRP on glassy carbon electrodes by Moyo et al . In another study, Chekin et al .…”

“…Jiang et al used chitosan-wrapped single-walled carbon nanotubes (SWCNTs) for direct bioelectrochemical sensing of H 2 O 2 using the HRP enzyme without any mediator. 14 Maize tassel and multiwalled carbon nanotube composite materials were also used as an adsorption matrix for HRP on glassy carbon electrodes by Moyo et al 15 In another study, Chekin et al recently demonstrated the adsorption of HRP on carboxyl-functionalized SWCNTs. 16 More recently peptide nanotubes were used for the amperometric mediatorless detection of H 2 O 2 using the HRP enzyme as well.…”