A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs

Skardal, Aleksander; Devarasetty, Mahesh; Kang, Hyun Wook; Mead, Ivy; Bishop, Colin E.; Shupe, Thomas; Lee, Sang Jin; Jackson, John D.; Yoo, James J.; Söker, Shay; Atala, Anthony

doi:10.1016/j.actbio.2015.07.030

Cited by 372 publications

(331 citation statements)

References 36 publications

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“…[123] These modifications enable tighter control over both the printability of a gel and its ability to match the properties of the tissue being printed in the pursuit of more biomimetic constructs. Composite hydrogels [124] and decellularized extracellular matrix hydrogels [121,125] further expand the range of material and biochemical properties available for bioprinting. The designs for bioprinted tissues may be a carefully engineered shape, or a personalized 3D reconstruction extracted from a patient’s imaging data (e.g., computed tomography and magnetic resonance imaging).…”

Section: Engineering Organ-specific Tissue and Functional Unitsmentioning

confidence: 99%

Tissue engineering toward organ-specific regeneration and disease modeling

2017

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Tissue engineering has been recognized as a translational approach to replace damaged tissue or whole organs. Engineering tissue, however, faces an outstanding knowledge gap in the challenge to fully recapitulate complex organ-specific features. Major components, such as cells, matrix, and architecture, must each be carefully controlled to engineer tissue-specific structure and function that mimics what is found in vivo. Here we review different methods to engineer tissue, and discuss critical challenges in recapitulating the unique features and functional units in four major organs—the kidney, liver, heart, and lung, which are also the top four candidates for organ transplantation in the USA. We highlight advances in tissue engineering approaches to enable the regeneration of complex tissue and organ substitutes, and provide tissue-specific models for drug testing and disease modeling. We discuss the current challenges and future perspectives toward engineering human tissue models.

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Section: Engineering Organ-specific Tissue and Functional Unitsmentioning

confidence: 99%

Tissue engineering toward organ-specific regeneration and disease modeling

2017

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“…They also serve as potential in vitro model systems for screening drugs, predicting cancer metastasis 158,159 and for elucidation of biological mechanisms.…”

Section: Alternative Bottom-up Approachesmentioning

confidence: 99%

Approaches for building bioactive elements into synthetic scaffolds for bone tissue engineering

Kesireddy

Kasper

2016

J. Mater. Chem. B

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Bone tissue engineering (BTE) is emerging as a possible solution for regeneration of bone in a number of applications. For effective utilization, BTE scaffolds often need modifications to impart biological cues that drive diverse cellular functions such as adhesion, migration, survival, proliferation, differentiation, and biomineralization. This review provides an outline of various approaches for building bioactive elements into synthetic scaffolds for BTE and classifies them broadly under two distinct schemes; namely, the top-down approach and the bottom-up approach. Synthetic and natural routes for top-down approaches to production of bioactive constructs for BTE, such as generation of scaffold-extracellular matrix (ECM) hybrid constructs or decellularized and demineralized scaffolds, are provided. Similarly, traditional scaffold-based bottom-up approaches, including growth factor immobilization or peptide-tethered scaffolds, are provided. Finally, a brief overview of emerging bottom-up approaches for generating biologically active constructs for BTE is given. A discussion of the key areas for further investigation, challenges, and opportunities is also presented.

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“…In situ skin printing is challenging as it requires the development of dedicated bioprinting devices, the integration with imaging systems to obtain data from the wound site, and the design of bioinks capable of instructing printed cells to perform Cell liver spheroids Extrusion Liver [185] their native functions. Binder et al [18,184] developed a device for the in situ skin printing composed of a cartridge delivery system containing a series of inkjet nozzles and a laser scanning system, both mounted on a portable XYZ plotting system.…”

Section: Printed Skin Constructs For Wound Healingmentioning

confidence: 99%

“…In these processes, the material to be printed, known as bioink, is loaded in a reservoir and subsequently deposited onto a receiving substrate through the action of light, pressurized air, vibration, thermal or mechanical effects. Bioinks consist of hydrogel precursor solutions or decellularized extracellular matrix loadable with cells and/or bioactive factors that play a pivotal role on the overall reproducibility of the printing process and quality of the printed construct [100,143,169,185]. Depending on the bioprinting technique, bioinks can be Drawbacks Mechanical stresses generated during the bioink deposition; difficult to produce hierarchical 3D constructs with intricate geometries deposited as small droplets (inkjet and laser-assisted technologies) or continuous strands of material (extrusionbased technologies), resulting in different printing times and constructs with distinct levels of heterogeneity, resolution and accuracy [111,125].…”

Section: Bioprinting Technologiesmentioning

confidence: 99%

“…In this versatile technology, cell-laden polymeric solutions [28,154], decellularized ECM components [73], cell suspensions [68], microcarriers [162] or tissue spheroids [185] are loaded into standard disposable syringes, and printed onto a building platform driven by pneumatic (pressurized air), mechanical (piston or screw) or solenoid (electrical pulses)-based dispensing systems [76,111,125,137]. An important advantage of extrusion bioprinting is the ability to print highly viscous polymer solutions containing a wide range of cell densities (up 10 7 cells mL -1 ) [92,182].…”

Section: Extrusion Bioprintingmentioning

confidence: 99%

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Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs

Cited by 372 publications

References 36 publications

Tissue engineering toward organ-specific regeneration and disease modeling

Tissue engineering toward organ-specific regeneration and disease modeling

Approaches for building bioactive elements into synthetic scaffolds for bone tissue engineering

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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