A human Y-chromosomal DNA sequence expressed in testicular tissue

Arnemann, J.; Epplen, Jörg T.; Cooke, Howard J.; Sauermann, Ulrike; Engel, W. King; Schmidtke, Jörg

doi:10.1093/nar/15.21.8713

Cited by 88 publications

(52 citation statements)

References 32 publications

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“…7,26 In this report, we have described the differential repopulating potential of human CD34 À Lin À and CD34 + Lin À repopulating stem cells in the same recipient using cotransplantation of male and female cells into the NOD/SCID EMV null mouse. 5,7,19 In effect, this strategy provides starting populations of 'Y-chromosome-marked' cells of a given phenotype that can be tracked in vivo in the presence of other phenotypically distinct stem cell subsets. This approach permits the prospective isolation of candidate stem cell populations and explores the interplay of distinct stem cell populations and their contribution to repopulation without the added complications of inefficient gene marking procedures.…”

Section: Discussionmentioning

confidence: 99%

“…The presence or absence of human DNA was confirmed by PCR (Gene Amp s PCR System 9700, PerkinElmer, Norwalk, CT, USA) for an untranslated region of the human-specific CART-1 gene as shown previously. 19 The criterion for human engraftment was the presence of 40.1% human DNA in the BM of transplanted mice by Southern blot and a positive CART-1 PCR. Analysis of human-male cell contribution too was determined by Southern blot and PCR for the human Y-chromosome-specific sequence DYS14.…”

Section: Flow Cytometric Analysis Of Murine Bmmentioning

confidence: 99%

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Coculture and transplant of purified CD34+Lin− and CD34−Lin− cells reveals functional interaction between repopulating hematopoietic stem cells

et al. 2003

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The human hematopoietic stem cell compartment is comprised of repopulating CD34 + and CD34 À cells. The interaction between these subsets with respect to their reconstitution capacity in vivo remains to be characterized. Here, lineagedepleted (Lin À ) human CD34 + and CD34 À hematopoietic cells were isolated from human male and female umbilical cord blood (CB) and transplanted into immune-deficient NOD/SCID EMV null mice, thereby allowing the use of human and Ychromosome-specific DNA sequences to discriminate human reconstitution contributed by CD34 + vs CD34 À repopulating stem cells. Although cultured human CB CD34 À Lin À cells transplanted alone possessed only minimal repopulating capacity, with 15% of mice achieving low levels of engraftment, transplantation of cocultured male CD34 À Lin À cells with female CD34 + Lin À cells demonstrated human repopulation with a contribution from CD34 À Lin À -derived progeny in 80% of the recipients. After coculture and transplantation, male CD34 À Lin À cells gave rise to primitive CD34 + CD38 À cells isolated in vivo, which demonstrated clonogenic progenitor function into multiple lineages. Taken together, our study indicates that the presence of CD34 + Lin À cells in coculture enhanced the low repopulating function of human CD34 À Lin À cells in vivo. We propose that CD34 + Lin and CD34 À Lin cells represent phenotypically distinct, but related cell types that exhibit unique and previously unappreciated functional interaction.

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Section: Discussionmentioning

confidence: 99%

Section: Flow Cytometric Analysis Of Murine Bmmentioning

confidence: 99%

Coculture and transplant of purified CD34+Lin− and CD34−Lin− cells reveals functional interaction between repopulating hematopoietic stem cells

et al. 2003

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“…1 ). The number of copies varies in the population from 35 to 60, including pseudogenes 2 . The majority of copies are situated on Yp11.2.…”

Section: Tspy Genementioning

confidence: 99%

Sequence Recombination in Exon 1 of the Tspy Gene in Men With Impaired Fertility

Svačinová¹,

Vodička²,

Vrtěl³

et al. 2011

BIOMED PAP

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Aim. The aim of this study was to evaluate TSPY (testis specific protein on the Y chromosome) gene and 5'UTR (UnTranslated Region) polymorphisms in men with impaired fertility compared to fertile controls.Methods. We analyzed 72 infertile men and 31 fertile controls usingconventional sequencing analysis to find crucial SNPs (single nucleotide polymorphism) and other changes.Results. The most remarkable changes were found in the 1 st exon only. In one half of the both infertile men and fertile controls, the most frequent finding was 26 SNPs with a similar pattern. In the other half we found highly relevant changes, generating a stop codon in the first third of exon 1. Early termination cut down the protein by 78.5%. This kind of change was not found in the fertile controls. No correlation was found between the spermiogram and the changes leading to the stop codon. The distribution of men with deletions, insertion and higher gene copy number was not statistically different.Conclusion. The changes found in exon 1 in infertile men could fundamentally affect the process of spermatogenesis. These findings could significantly enhance our understanding of the molecular-genetic causes of male infertility.

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“…All the samples analyzed after the third year from BMT were negative, as and 911, of the DYS14 sequence. 4,19 The PCR amplification of DYS14 yields a PCR product of 239 bp in male cells.…”

Section: Sample Collection and Dna Extractionmentioning

confidence: 99%

Long-term persistence of hemopoietic chimerism following sex-mismatched bone marrow transplantation

Mangioni

Balduzzi

Rivolta

et al. 1997

Bone Marrow Transplant

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Summary:of donor origin are detectable, or as mixed chimeras if a mixture of both donor and recipient origin cells are found after BMT. With the use of increasingly sensitive techFifty-eight samples of bone marrow (31), whole peripheral blood (8) and separated fractions of circulating niques, mixed chimerism is frequently observed after allogeneic BMT. [2][3][4][5][6] Several factors related to transplant promononuclear (11) and polymorphonuclear (8) cells from 18 male patients, transplanted for hematological discedures (type of donor, conditioning regimens, T cell depletion, graft-versus-host disease (GVHD) prophylaxis) eases from related (14) or unrelated (4) female donors were analyzed for chimerism at subsequent intervals and patient features (underlying disease, age) have been shown to be associated with an increase in mixed chimer-(range, 1-72 months) following bone marrow transplantation, by means of PCR amplification of the Y-chromism (MC) after sibling BMT. [7][8][9][10][11][12] Residual male cells were shown to persist in patients grafted with T cell non-depleted osome-specific DYS14 sequence, revealed by a radiolabelled hybridization probe (dot blot technique, 0.01% marrow from an HLA-identical female donor, when clinical and hematological conditions indicated a complete sensitivity). Detection of male cells was positive in all but two of 52 samples collected within the third year engraftment and the absence of recurrent disease. 4,5,13 The need to extend the analysis of MC following BMT to a after transplantation and negative in six samples collected from three patients after the third year. In the larger series of prospectively evaluated patients with adequate follow-up, prompted us to investigate the persistfirst year after transplantation, mixed chimerism was found in all patients, apparently with no correlation ence of residual recipient cells in a series of pediatric male patients grafted from a female donor. Our findings on seriwith graft-versus-host disease. Comparable results were found in fractions of mononuclear and polymorphoally collected samples from patients in long-term continuous complete remission (CCR) establish the kinetics of the nuclear cells, when analyzed separately. The persistence of very low levels of recipient cells in patients in conresidual recipient cells, with no apparent correlation with post-transplant events. tinuous complete remission until the third year after transplantation, suggests the persistence of normal host hemopoiesis for a long period of time after the so-called myeloablative regimen. The progressive negativization, Patients and methods occurring in our patients between the second and the fourth year after transplantation, could signify the disPatients appearance of residual host hemopoiesis or its decrease to below the detection level of this highly sensitive Eighteen male patients who received a marrow graft from familial (14) or unrelated (four) female donor at the Pedimethod. Keywords: chimerism; bone marrow transplantation; dot atric Department of ...

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A human Y-chromosomal DNA sequence expressed in testicular tissue

Cited by 88 publications

References 32 publications

Coculture and transplant of purified CD34+Lin− and CD34−Lin− cells reveals functional interaction between repopulating hematopoietic stem cells

Coculture and transplant of purified CD34+Lin− and CD34−Lin− cells reveals functional interaction between repopulating hematopoietic stem cells

Sequence Recombination in Exon 1 of the Tspy Gene in Men With Impaired Fertility

Long-term persistence of hemopoietic chimerism following sex-mismatched bone marrow transplantation

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