A human adenoviral vector with a chimeric fiber from canine adenovirus type 1 results in novel expanded tropism for cancer gene therapy

Stoff-Khalili, Mariam A.; Rivera, Angel A.; Glasgow, Joel N.; Le, Long P.; Stoff, Alexander; Everts, Maaike; Tsuruta, Yuko; Kawakami, Yosuke; Bauerschmitz, Gerd; Mathis, J. Michael; Pereboeva, Larisa; Seigal, G. P.; Dall, P.; Curiel, David T.

doi:10.1038/sj.gt.3302588

Cited by 39 publications

(39 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since OS in dogs is virtually identical to human OS this is a valuable model to increase our understanding of sarcomas translate CRAd effectivety to the human situation and provides a unique immunocompetent context. We have advanced our work in this area by demonstrating that chimeric fiber derived from both Canine Adenovirus Types 1 and 2-display novel tropism (43,137). Furthermore, we have shown that the canine adenovirus can be fluorescently tagged for use in imaging studies (81) and that novel constructs result in infectivity enhancement of canine OS cells (82).…”

Section: Promoter-driven Viral Replicationmentioning

confidence: 87%

Evolving gene therapy approaches for osteosarcoma using viral vectors: Review

Witlox

Lamfers

Wuisman

et al. 2007

Bone

View full text Add to dashboard Cite

Section: Promoter-driven Viral Replicationmentioning

confidence: 87%

Evolving gene therapy approaches for osteosarcoma using viral vectors: Review

Witlox

Lamfers

Wuisman

et al. 2007

Bone

View full text Add to dashboard Cite

“…Studies have shown that canine Ad 2 knob binds to CAR but the presence of recombinant Ad5 knob only partially blocks this binding [19,28]. Also, CK1 appears to display CAR-independent infection [19]. Thus, Ad5 with canine Ad knob domains may not be completely CAR-independent.…”

Section: Resultsmentioning

confidence: 99%

“…Whereas infection with Ad5Luc1-CK1 and Ad5Luc1-CK2 increased infectivity in HUVEC 100 and 200-fold respectively, the exact mechanisms of binding and internalization of these canine Ads are not yet fully characterized. Studies have shown that canine Ad 2 knob binds to CAR but the presence of recombinant Ad5 knob only partially blocks this binding [19,28]. Also, CK1 appears to display CAR-independent infection [19].…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we evaluated Ad5 vectors with the following capsid modifications that have been previously described: incorporation of the integrin-binding RGD peptide (Ad5.RGD) and a poly-lysine (Ad5.pK7) motif into the HI loop of fiber [16,17], human adenovirus serotype 3 knob domain (Ad5/3Luc1) [18], and canine adenovirus serotype 1 or 2 knob domains (Ad5Luc1-CK1 and Ad5Luc1-CK2) [19,20]. The RGD and pK7 Ad5 vectors contain a dual reporter cassette that contains luciferase and green fluorescent protein.…”

Section: Tropism-modified Adenoviral Vectorsmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Gene Delivery to Human Primary Endothelial Cells Using Tropism-Modified Adenovirus Vectors

Preuss¹,

Glasgow²,

Everts³

et al. 2008

TOGTJ

View full text Add to dashboard Cite

Endothelial cells have been noted to have relatively low expression of the native receptor for adenovirus serotype 5 (Ad5), coxsackie and adenovirus receptor (CAR), and are thus refractory to Ad5 infection. In this study, we hypothesize that increases in the infectivity of Ad5 in primary human pulmonary artery (HPAEC), coronary artery (HCAEC) and umbilical vein endothelial cells (HUVEC) can be achieved through genetic capsid modification of Ad5 to bypass CAR-dependent infection. The modifications tested in this study include incorporation of an integrin-binding RGD peptide motif (Ad5.RGD), a poly-lysine motif (Ad5.pK7), a combination of both of these peptide domains (Ad5.RGD.pK7), an adenovirus serotype 3 knob domain (Ad5/3Luc1) and canine adenovirus serotype 1 or 2 knob domains (Ad5Luc1-CK1 and Ad5Luc1-CK2). In HPAEC and HCAEC, the greatest infectivity enhancements were achieved using Ad5/3Luc1 (26-fold and 30-fold respectively). HUVEC was most readily infected by Ad5Luc1-CK1 (213-fold). These results demonstrate that gains in Ad5 infectivity in endothelial cells can be accomplished with genetic capsid modifications.

show abstract

“…[71][72][73] This fiber pseudotyping approach has identified chimeric vectors with superior infectivity to Ad5 in several clinically relevant cell types, including primary ovarian carcinoma cells, [74][75][76] 81 human cardiovascular tissue 82 and others. 83,84 Interestingly, this strategy has been extended to exploit fiber elements from non-human Ads 85,86 and the fiber-like s1 reovirus attachment protein, which targets cells expressing junctional adhesion molecule. 87,88 Direct ligand incorporation into the Ad knob domain without ablating native CAR-binding has resulted in Ad vectors with expanded, rather than restricted, cell recognition.…”

Section: Adenovirus Targeting Via Genetic Modification: Fibermentioning

confidence: 99%