A hot water extract ofCurcuma longainhibits adhesion molecule protein expression and monocyte adhesion to TNF-α-stimulated human endothelial cells

Abstract

The recruitment of arterial leukocytes to endothelial cells is an important step in the progression of various inflammatory diseases. Therefore, its modulation is thought to be a prospective target for the prevention or treatment of such diseases. Adhesion molecules on endothelial cells are induced by proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), and contribute to the recruitment of leukocytes. In the present study, we investigated the effect of hot water extract of Curcuma longa (WEC) on the protein expression of adhesion molecules, monocyte adhesion induced by TNF-α in human umbilical vascular endothelial cells (HUVECs). Treatment of HUVECs with WEC significantly suppressed both TNF-α-induced protein expression of adhesion molecules and monocyte adhesion. WEC also suppressed phosphorylation and degradation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) induced by TNF-α in HUVECs, suggesting that WEC inhibits the NF-κB signaling pathway.

A hot water extract of turmeric (Curcuma longa) suppresses acute ethanol-induced liver injury in mice by inhibiting hepatic oxidative stress and inflammatory cytokine production

“…Our previous study provided evidence that the anti-inflammatory activity of WEC, which inhibits the NF-κB signalling pathway, is partly due to a component called bisacurone ( 22 ) . Bisacurone has been found to suppress elevation of ROS, activation of NF-κB and expression of vascular cell adhesion molecules ( 17 ) .…”

“…WEC was prepared according to the method described previously ( 22 ) . In brief, rhizomes of turmeric ( Curcuma longa Linn.)…”

“…For example, aqueous extracts of turmeric have been reported to exhibit antioxidant activity ( 16 ) and anti-inflammatory activity ( 17 ) , as well as promoting corneal wound healing ( 18 ) , an antidepressant effect ( 19 ) , an anticancer effect ( 20 ) and regulating cytochrome P450 (CYP) activity ( 21 ) . We recently reported that a hot water extract of C. longa (WEC) modulates the adhesive properties of endothelial cells by suppressing TNF-α-induced expression of cell adhesion molecules via inhibition of the NF-κB signalling pathway ( 22 ) . These effects of WEC are at least partly attributable to bisacurone, a component of turmeric that has both antioxidant and anti-inflammatory activities ( 17 , 23 ) .…”

Effects of hot water extract of Curcuma longa on human epidermal keratinocytes in vitro and skin conditions in healthy participants: A randomized, double‐blind, placebo‐controlled trial

“…UVB upregulates inflammatory cytokine expression through activation of nuclear factor kappaB (NF‐κB), a transcription factor . We previously reported that WEC inhibited VCAM‐1 expression by inhibiting NF‐κB activation in human umbilical vein endothelial cells . Therefore, WEC may inhibit UVB‐induced upregulation of TNF‐α and IL‐1β through inhibition of NF‐κB activation.…”

“…Curcuma longa is a widely used spice that possesses various biological activities . For example, aqueous extracts of C longa have been reported to exhibit antioxidant and anti‐inflammatory activities, to promote corneal wound healing, and to have antidepressant and an anticancer effects . We recently reported that a hot water extract of C longa (WEC) suppresses acute ethanol‐induced liver injury by inhibiting hepatic oxidative stress and inflammatory cytokine production .…”