A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France

Menu, Estelle; Mary, Charles; Toga, I.; Raoult, Didier; Ranque, Stéphane; Bittar, Fadi

doi:10.1017/s0950268819000165

Cited by 14 publications

(16 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our survey revealed a high rate of Balantidium coli infection in gorillas from the Republic of the Congo and captive gorillas from France, at 57.9% and 77.8%, respectively ( Table 1 ). B. coli is a cosmopolitan ciliate that colonizes the intestines of many animals, including humans, with pigs serving as reservoir hosts [ 6 , 14 ]. In captive gorillas, infections with B. coli are usually without clinical signs.…”

Section: Discussionmentioning

confidence: 99%

“…DNA extraction was performed using the EZ1 (Qiagen GmbH, Hilden, Germany) automated protocol with pretreatment [ 14 ]. The extraction protocol was adapted for stool processing as follows: 200 mg of stool sample was added to 350 µL of G2 lysis buffer (Qiagen GmbH, Hilden, Germany) in a tube containing glass powder and then disrupted in a FastPrep-24 grinder (MP Biomedicals) at maximum power for 40 s. After 10 min of incubation at 100 °C to allow complete lysis, the tubes were centrifuged at 10,000 g for one minute.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations

Davoust

Mediannikov

et al. 2021

Pathogens

Self Cite

View full text Add to dashboard Cite

Non-human primate populations act as potential reservoirs for human pathogens, including viruses, bacteria and parasites, which can lead to zoonotic infections. Furthermore, intestinal microorganisms may be pathogenic organisms to both non-human primates and humans. It is, therefore, essential to study the prevalence of these infectious agents in captive and wild non-human primates. This study aimed at showing the prevalence of the most frequently encountered human enteric protozoa in non-human primate populations based on qPCR detection. The three populations studied were common chimpanzees (Pan troglodytes) in Senegal and gorillas (Gorilla gorilla) in the Republic of the Congo and in the Beauval Zoo (France). Blastocystis spp. were mainly found, with an occurrence close to 100%, followed by Balantidiumcoli (23.7%), Giardiaintestinalis (7.9%), Encephalitozoonintestinalis (1.3%) and Dientamoebafragilis (0.2%). None of the following protozoa were detected: Entamoebahistolytica, Enterocytozoonbieneusi, Cryptosporidiumparvum, C. hominis, Cyclosporacayetanensis or Cystoisosporabelli. As chimpanzees and gorillas are genetically close to humans, it is important to monitor them frequently against different pathogens to protect these endangered species and to assess potential zoonotic transmissions to humans.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations

Davoust

Mediannikov

et al. 2021

Pathogens

Self Cite

View full text Add to dashboard Cite

show abstract

“…is thus much lower in industrialized countries than in lower-income geographical areas [ 5 ]. For instance, it reaches 10 to 20% in European countries [ 8 , 9 , 10 , 11 ] but can largely exceed 40–50% in various African [ 12 , 13 ], Asian [ 14 , 15 ], and American countries [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Subtype Distribution of Blastocystis sp. in Senegalese School Children

Khaled

Gantois

et al. 2020

Microorganisms

View full text Add to dashboard Cite

Blastocystis sp. is an enteric protozoan that frequently colonizes humans and many animals. Despite impacting on human health, data on the prevalence and subtype (ST) distribution of Blastocystis sp. remain sparse in Africa. Accordingly, we performed the first multicenter and largest epidemiological survey ever conducted on Blastocystis sp. for this continent. A total of 731 stool samples collected from healthy school children living in 10 villages of the northwestern region of Senegal were tested for the presence of Blastocystis sp. by real-time polymerase chain reaction followed by subtyping of positive samples. Considerable variation in prevalence between villages (51.7 to 100%) was evident with the overall prevalence being 80.4%. Mixed infections were identified in 23% of positive individuals. Among 453 school children with a single infection, ST2 was predominant, followed by ST1, ST3, ST7, ST10, and ST14; this is the first report of ST10 and ST14 in humans. Genetic polymorphisms were evident at the intra-ST level with the identification of numerous ST1 to ST3 genotypes. ST1 showed the greatest intra-ST diversity followed by ST2 and ST3. The prevalence and distribution of STs and genotypes varied among target villages, pointing to several potential infection sources, including human-to-human, zoonotic, and waterborne transmission.

show abstract

“…KEYWORDS GEMS, Giardia, MAL-ED, database, diarrhea, microbiome, parasite E nteric parasites, including helminths and protozoa, are among the most prevalent infections in lower-and middle-income countries (LMICs) with an estimated 3.5 billion people affected worldwide (1,2). Infection with eukaryotic pathogens often results in acute, moderate to severe diarrheal disease and/or chronic malnutrition and stunting, which has significant consequences for morbidity and mortality (3)(4)(5).…”

mentioning

confidence: 99%

Natural Infection with Giardia Is Associated with Altered Community Structure of the Human and Canine Gut Microbiome

Berry

Johnson

Martins

et al. 2020

mSphere

View full text Add to dashboard Cite

Enteric parasitic infections are among the most prevalent infections in lower- and middle-income countries (LMICs) and have a profound impact on global public health. While the microbiome is increasingly recognized as a key determinant of gut health and human development, the impact of naturally acquired parasite infections on microbial community structure in the gut, and the extent to which parasite-induced changes in the microbiome may contribute to gastrointestinal symptoms, is poorly understood. Enteric parasites are routinely identified in companion animals in the United States, presenting a unique opportunity to leverage this animal model to investigate the impact of naturally acquired parasite infections on the microbiome. Clinical, parasitological, and microbiome profiling of a cohort of 258 dogs revealed a significant correlation between parasite infection and composition of the bacterial community in the gut. Relative to other enteric parasites, Giardia was associated with a more pronounced perturbation of the microbiome. To compare our findings to large-scale epidemiological studies of enteric diseases in humans, a database mining approach was employed to integrate clinical and microbiome data. Substantial and consistent alterations to microbiome structure were observed in Giardia-infected children. Importantly, infection was associated with a reduction in the relative abundance of potential pathobionts, including Gammaproteobacteria, and an increase in Prevotella—a profile often associated with gut health. Taken together, these data show that widespread Giardia infection in young animals and humans is associated with significant remodeling of the gut microbiome and provide a possible explanation for the high prevalence of asymptomatic Giardia infections observed across host species. IMPORTANCE While enteric parasitic infections are among the most important infections in lower- and middle-income countries, their impact on gut microbiota is poorly understood. We reasoned that clinical symptoms associated with these infections may be influenced by alterations of the microbiome that occur during infection. To explore this notion, we took a two-pronged approach. First, we studied a cohort of dogs naturally infected with various enteric parasites and found a strong association between parasite infection and altered gut microbiota composition. Giardia, one of the most prevalent parasite infections globally, had a particularly large impact on the microbiome. Second, we took a database-driven strategy to integrate microbiome data with clinical data from large human field studies and found that Giardia infection is also associated with marked alteration of the gut microbiome of children, suggesting a possible explanation for why Giardia has been reported to be associated with protection from moderate to severe diarrhea.

show abstract

A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France

Cited by 14 publications

References 44 publications

Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations

Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations

Prevalence and Subtype Distribution of Blastocystis sp. in Senegalese School Children

Natural Infection with Giardia Is Associated with Altered Community Structure of the Human and Canine Gut Microbiome

Contact Info

Product

Resources

About