A Honeycomb Collagen Carrier for Cell Culture as a Tissue Engineering Scaffold

Itoh, Hiroshi; Aso, Yu; Furuse, Masayasu; Noishiki, Yasuharu; Miyata, Teruo

doi:10.1046/j.1525-1594.2001.025003213.x

Cited by 90 publications

(69 citation statements)

References 15 publications

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“…Among the widely used natural biopolymers, collagen has been conventionally applied in the clinic in various forms, such as gels or sponges, since it shows good biocompatibility and absorbability. Atelocollagen molecules do not contain telopeptides, and show extremely low antigenicity when adopted as basic materials for medical use 16) . Schlegel et al 17) reported that transplantation of bovine collagen alone into mini-pig skull defects showed higher degrees of de novo bone formation comparable with that of autogenous bone grafts.…”

Section: Discussionmentioning

confidence: 99%

Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles

et al. 2011

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We evaluated the effects on bone formation of combining synthesized collagen model polypeptides consisting of a Pro-Hyp-Gly [poly(PHG)] sequence and alpha-tricalcium phosphate (-TCP) particles with various median sizes (large: 580.8 µm; small: 136.2 µm; or large and small mixed: 499.3 µm) in a skull defect model in mini-pigs. Quantitative image analyses for the volume density (VD) of new bone revealed that the VD in each -TCP group was significantly higher than that in the poly(PHG) control group, with the mixed group showing the highest VD among all the groups at 4 weeks after implantation. Histological assessments revealed that the small -TCP particles were almost completely degraded at 8 weeks. At 12 weeks, all sizes of -TCP particles were completely degraded and remodeling of the lamellar bone was observed. The present findings suggest that particle size may influence the success of bone formation in defects.

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Section: Discussionmentioning

confidence: 99%

Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles

et al. 2011

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“…[26][27][28][29] . We selected atelocollagen honeycomb scaffold as a carrier, because it offers a good environment for the cells and good mechanical stability, and maintains its original size and shape during cell growth in vitro 17) . Importantly, KUSA/A1 cells on Atelocollagen scaffold were able to proliferate and differentiate into osteoblasts, followed by the degradation of the scaffold.…”

Section: Quantification Of Bone Induction Vessel Formation and Cellumentioning

confidence: 99%

“…It is even used to minimize allergic reactions in cosmetics 16) . In the present study, we selected atelocollagen type I honeycomb scaffold because it has high cell affinity, and maintains its structural integrity, original size and shape during cell growth in vitro 17) .…”

Section: Introductionmentioning

confidence: 99%

Biological Analysis of a Candidate Stem Cell-KUSA/A1 cell-for Bone Tissue Engineering

Rodrı́guez

Tsujigiwa

Borkosky

et al. 2007

J. Hard Tissue Biology.

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Abstract:The basic principle of bone induction for tissue engineering is to use stem cells, growth factors and organic matrix. KUSA/A1 cell is an example of bone marrow stromal stem cell, capable of differentiating into osteoblasts, chondrocytes and myotubes under inducing conditions. It has been reported that mature KUSA/A1 osteoblasts cultured in osteogenic condition, were able to induce a few bone formation in collagen hybridized PLGP sponge in vivo. This may be due to their low proliferation potential thereby not being able to obtain sufficient number of cells to promote large tissue repair.Because of this, in order to use KUSA/A1 cells with high cell proliferation activity to induce large amount of new bone, we evaluated whether KUSA/A1 cells in non-induction condition will maintain their immature stage. The result demonstrated that KUSA/A1 cells cultured in α-MEM maintained their immature stage in vitro. We further examined the osteoblastic differentiation under the influence of the host microenvironment in intraperitoneal diffusion chamber. The results indicated that immature KUSA/A1 cells in vivo cell culture differentiated into osteoblasts and produced mineralized bone-like tissue. Finally, we evaluated the effect of honeycomb scaffold to produce abundant bone formation using KUSA/A1 cells implanted in subcutaneous tissues of SCID mice. 1x10 6 KUSA/A1 cells with honeycomb scaffold showed abundant new bone formation. While, 5x106 KUSA/A1 cells alone showed only few small islands of new bone formation. This study support that KUSA/A1 cell is a good candidate as stem cells for basic research in bone tissue engineering.

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“…Collagen sponges are considered to be a useful scaffold matrix for several tissues. And extra cellular matrix molecules such as laminin and fibronectin can be adsorbed onto the collagen scaffold, in order to aid cell migration and proliferation 9) . Furthermore, Type I collagen is the major component of the bone matrix and is useful as a carrier of osteoblasts 10) .…”

Section: Discussionmentioning

confidence: 99%

.BETA.TCP/Collagen Sponge Composite Enhances the Osteogenic Differentiation of Mesenchymal Stem Cells

Matsuno

Hashimoto

Nakahara

et al. 2005

J. Hard Tissue Biology.

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For the bone regeneration, tissue engineering approach combines cells capable of osteogenic activity with an appropriate scaffold. We developed a biodegradable sponge composite (b-TCP/CS) by combining â-tricalcium phosphate (â-TCP) granules and collagen. Human mesenchymal stem cells (MSCs) were cultured within b-TCP/CS scaffolds and collagen sponge (CS) scaffolds, we investigated the alkaline phosphates (ALP) activity and the expression of osteogenic markers using RT-PCR. Furthermore, MSCs-loaded b-TCP/CS and CS were implanted under the back skin of nude mice for 4 and 12 weeks, and then removed for histological evaluation of bone formation. In vitro studies demonstrated that b-TCP/CS with MSCs of the ALP activity and the expression of osteogenic markers was higher than CS. In vivo, no bone formation was observed in CS, but newly bone formation was observed around â-TCP granules of b-TCP/CS. These results suggest that b-TCP/ CS composites enhance the osteogenic differentiation of MSCs and new bone formation.

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A Honeycomb Collagen Carrier for Cell Culture as a Tissue Engineering Scaffold

Cited by 90 publications

References 15 publications

Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles

Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles

Biological Analysis of a Candidate Stem Cell-KUSA/A1 cell-for Bone Tissue Engineering

.BETA.TCP/Collagen Sponge Composite Enhances the Osteogenic Differentiation of Mesenchymal Stem Cells

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