A Histopathological Study of the Inner Ear after the Administration of Hyaluronan into the Middle Ear of the Guinea Pig

Bjurström, Sivert; Slepecky, Norma; Angelborg, C.

doi:10.3109/00016488709102841

Cited by 28 publications

(26 citation statements)

References 4 publications

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“…Either the substances are taken up into the blood vessels or lymphatics and transported into the perilymph, or they cross the inner epithelial layer to diffuse into the perilymph 9,10 . The passage of substances through this semipermeable membrane is affected by the membrane's morphologic integrity, the presence and the duration of the inflammation, and molecular weight, concentration, configuration, liposolubility, and electrical charge of the substances 10,11 …”

Section: Discussionmentioning

confidence: 99%

Effect Of Intratympanic Dexamethasone on Noise-Induced Temporary Threshold Shift

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Effect Of Intratympanic Dexamethasone on Noise-Induced Temporary Threshold Shift

et al. 2005

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“…Most of the histologic studies in the literature have shown that use of the viscous form of HA in the ME cavity has not caused either new bone or adhesions to form in the ME cavity. 6,7 The study 11 evaluating the "foam" esterified form of HA has reported new bone formation, mucosa thickness, and adhesions, although they were moderate when compared with the adverse effects caused by the use of AGS. The severity of pathologic change for both MeroGel and AGS might be dependent on the severity and extent of ME mucosa wounding as well as the position and amount of packing material applied within the ME cavity.…”

Section: Commentmentioning

confidence: 99%

“…Hyaluronic acid is a naturally occurring extracellular polysaccharide with a molecular weight in the range of 2 to 4 million daltons. 1 Although purified HA has been shown to have a high level of tissue biocompatibility and to be nontoxic to the inner ear, [6][7][8] its viscous physical properties 1 has limited its use as a surgical packing material for the ME cavity. Because MeroGel is an esterified form of HA, its physical properties are more like that of a sponge, which make it an ideal packing material for ME application.…”

Section: Commentmentioning

confidence: 99%

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Evaluation of Esterified Hyaluronic Acid as Middle Ear–Packing Material

Li¹,

Feghali²,

Dinces³

et al. 2001

Arch Otolaryngol Head Neck Surg

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Objective: To evaluate the efficacy of esterified hyaluronic acid (MeroGel) as a middle ear (ME)-packing material.Design: Randomized controlled trial.Material: Twenty-four guinea pigs.Intervention: Group 1, MeroGel-treated animals (n=10), bilateral wounding of ME mucosa with 5 of the animals receiving the MeroGel packing in the left ME and 5 of the animals receiving MeroGel in the right ME; group 2, absorbable gelatin sponge-treated animals (n=10), with the same experimental protocol as in group 1 except that the absorbable gelatin sponge was the packing material; group 3, untreated animals (n=4), unilateral wounding of the left ME mucosa in 2 animals and in 2 animals in the right ME, with no packing material. Auditory brainstem recordings were performed for all groups before the ME operation and 5 days and 6 weeks after the operation.Results: Auditory brainstem response recordings at postoperative day 5 showed that all ears with ME packing had hearing losses in the frequency range of 500 to 4000 Hz. The recovery of hearing acuity at postoperative week 6 was significantly better in group 1 (MeroGel-treated) guinea pigs compared with group 2 (the absorbable gelatin sponge-treated) animals. In group 2 animals, 20% of the packing material remained in the ME cavities and new bone formation was observed, while in group 1 animals, there was less packing material in the ME and no formation of new bone. Conclusions:MeroGel is a nonototoxic packing material with a high level of biocompatibility for ME mucosa; it is an effective supportive material following ME surgery and is easily expelled from the ME cavity.

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“…The passage of substances through the RWM is affected by the membrane's thickness, morphologic integrity, the presence and duration of inflammation, and the physicochemical characteristics of the substances. 13,14 Although the RWM is accessible in the middle-ear space, some potential therapeutic agents are unable to readily cross the RWM to the cochlea due to their properties, 15 which limits the treatment of inner ear disorders. Moreover, the direct contact of some therapeutic agents, such as hydrocortisone, with middle ear structures can elicit morphological changes in the RWM.…”

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confidence: 99%

Enhanced local bioavailability of single or compound drugs delivery to the inner ear through application of PLGA nanoparticles via round window administration

Cai¹,

Wen²,

Wen³

et al. 2014

IJN

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In this paper, the potential of poly( d , l -lactide- co -glycolide acid) (PLGA) nanoparticles (NPs) for carrying single or compound drugs traversing the round window membrane (RWM) was examined after the round window (RW) administration of different NPs to guinea pigs. First, coumarin-6 was incorporated into PLGA NPs as a fluorescent probe to investigate its ability to cross the RWM. Then, PLGA NPs with salvianolic acid B (Sal B), tanshinone IIA (TS IIA), and total panax notoginsenoside (PNS) including notoginsenoside R 1 (R 1 ), ginsenoside Rg 1 (Rg 1 ), and ginsenoside Rb 1 (Rb 1 ) were developed to evaluate whether NPs loaded with compound drugs would pass through the RWM and improve the local bioavailability of these agents. PLGA NPs loaded with single or compound drugs were prepared by the emulsification solvent evaporation method, and their particle size distribution, particle morphology, and encapsulation efficiency were characterized. In vitro release study showed sustained-release profiles of Sal B, TS IIA, and PNS from the NPs. The pharmacokinetic results showed that NPs applied to the RWM significantly improved drug distribution within the inner ear. The AUC 0– t of coumarin-6 in the perilymph (PL) following RW administration of NPs was 4.7-fold higher than that of coumarin-6 solution, and the C max was 10.9-fold higher. Furthermore, the AUC 0– t of R 1 , Rg 1 , and Rb 1 were 4.0-, 3.1-, and 7.1-fold greater, respectively, after the application of NPs compared to the compound solution, and the C max were, respectively, 14.4-, 10.0-, and 16.7-fold higher. These findings suggest that PLGA NPs with unique properties at the nanoscale dimensions have a powerful ability to transport single or compound drugs into the PL through the RWM and remarkably enhance the local bioavailability of the encapsulated drugs in the inner ear. The use of PLGA NPs as nanoscale delivery vehicles to carry drugs across the RWM may be a promising strategy for the treatment of inner ear diseases.

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A Histopathological Study of the Inner Ear after the Administration of Hyaluronan into the Middle Ear of the Guinea Pig

Cited by 28 publications

References 4 publications

Effect Of Intratympanic Dexamethasone on Noise-Induced Temporary Threshold Shift

Effect Of Intratympanic Dexamethasone on Noise-Induced Temporary Threshold Shift

Evaluation of Esterified Hyaluronic Acid as Middle Ear–Packing Material

Enhanced local bioavailability of single or compound drugs delivery to the inner ear through application of PLGA nanoparticles via round window administration

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