A histological study of the effect of saline and povidone-iodine infusions on the equine endometrium

Olsen, L.; Al-Bagdadi, F.; Richardson, Gavin F.; Archbald, L.F.; Braun, W; McCoy, Dorothy; Godke, R.A.; Titkemeyer, C.; Thompson, Donald L.

doi:10.1016/0093-691x(92)90186-u

Cited by 15 publications

(21 citation statements)

References 7 publications

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“…The greatest morphological changes induced by gentamicin in this study were seen in the secretory cells. Similar changes have also been observed in cows 12 , and humans 6,8 . This study indicates that the repeated gentamicin infusions destroyed a considerable number of endometrial apical surfaces.…”

supporting

confidence: 83%

Scanning Electron Microscopy Morphology of the Uterine Epithelium of Mares Infused with Gentamicin

Al-Bagdadi¹,

Eilts

Richardson³

et al. 2002

Microsc Microanal

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Veterinarians treated bacterial endometritis in mares using prostaglandins 1 , infusions of antiseptics 2 , antibiotics 3 and Gentamicin 4 . The effects of repeated intrauterine infusions of povidone iodine and gentamicin on the histopathology of the endometrium have been reported. 4-6 . The scanning electron microscope has been used to study the surface morphology of the uterus in human beings 7,8 , rabbit 9 , mares 10 , and rats 11 . The purpose of this scanning electron microscopic study was to examine, if repeated intrauterine infusion of mares with gentamicin or with saline alone would change the surface morphology of the mares' uterine epithelium.

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supporting

confidence: 83%

Scanning Electron Microscopy Morphology of the Uterine Epithelium of Mares Infused with Gentamicin

Al-Bagdadi¹,

Eilts

Richardson³

et al. 2002

Microsc Microanal

Self Cite

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“…In general, an inflammatory endometrial reaction of healthy oestrous mares to any intrauterine infusion has to be considered physiologic (Olsen et al. ; Palm et al. ; LeBlanc ).…”

Section: Discussionmentioning

confidence: 99%

“…Intrauterine manipulation always results in an endometrial response and possible contamination with bacteria (Olsen et al. ); however, in the present study, intrauterine fluid accumulation was assessed, and uterine cultures were performed to evaluate for any effects of NAC on uterine clearance in comparison with a saline‐induced endometrial reaction.…”

Section: Introductionmentioning

confidence: 94%

Investigations on the Endometrial Response to Intrauterine Administration of N‐Acetylcysteine in Oestrous Mares

Melkus

Witte

Walter

et al. 2012

Reprod Domestic Animals

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In mares, mating-induced persistent endometritis contributes to low fertility. The condition is in part related to delayed clearance of mucus accumulated within the uterine lumen. The objective of this study was to investigate the endometrial response of healthy mares to intrauterine (i.u.) treatment with N-acetylcysteine (NAC). Oestrous mares (n = 12) were randomly assigned to a treatment (TM) or control (C) group and received an i.u. infusion of 5% NAC and saline (total volume 140 ml), respectively. Endometrial biopsies were collected in five of the mares 24 h after treatment, in the remaining seven mares 72 h after treatment. Endometrial biopsies were evaluated for integrity of the luminal epithelium, number of polymorphonuclear neutrophils (PMN), staining for cyclooxygenase 2 (COX2), staining with Kiel 67 antigen (Ki-67), lectins and periodic acid-Schiff (PAS). The integrity of endometrial epithelial cells was not affected by treatment (no statistical differences between groups or times). At 24 h after treatment, the mean number of PMN in endometrial biopsies from NAC- and C-mares did not differ, but at 72 h after treatment, number of PMN was significantly higher (p < 0.05) in C (3.9 ± 0.6 PMN/field) compared with NAC-treated mares (2.3 ± 0.2 PMN/field). At 72 h after treatment, the intensity of staining for COX2 was significantly higher after saline than after NAC treatment (p < 0.05). In the epithelium, no differences in staining for the proliferation marker Ki-67 were seen with respect to time and treatment. Score for the lectin wheat germ agglutinin (WGA) was slightly higher in NAC-treated mares than in C-mares 72 h after treatment (p < 0.05). Score for PAS staining of mucus in deep uterine glands differed significantly between groups at 24 h after treatment (p < 0.05). The present study demonstrates that NAC does not adversely affect the endometrial function. Moreover, an anti-inflammatory effect on the equine endometrium was observed.

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“…Normal saline has been shown to initiate a mild endometritis [141,142]. Resultant prostaglandin production stimulates uterine myometrial activity and evacuation of endometrial glands.…”

Section: Counterirritant Therapymentioning

confidence: 99%

“…Infusion of iodine preparations resulted in an acute inflammatory reaction in the superficial epithelial layer of the endometrium characterized by neutrophilia that lasted for up to 10 days and continuing as a chronic predominantly mononuclear cell reaction for up to 30 days [141]. Fibrosis may actually be increased after treatment [145].…”

Section: Counterirritant Therapymentioning

confidence: 99%

Reproductive Tract Infections in Horses

Lu¹,

Morresey²

2006

Veterinary Clinics of North America: Equine Practice

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A histological study of the effect of saline and povidone-iodine infusions on the equine endometrium

Cited by 15 publications

References 7 publications

Scanning Electron Microscopy Morphology of the Uterine Epithelium of Mares Infused with Gentamicin

Scanning Electron Microscopy Morphology of the Uterine Epithelium of Mares Infused with Gentamicin

Investigations on the Endometrial Response to Intrauterine Administration of N‐Acetylcysteine in Oestrous Mares

Reproductive Tract Infections in Horses

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