A histochemical study of ultraviolet B irradiation andOriganum hypericifoliumoil applied to the skin of mice

Abstract

Ultraviolet (UV) rays cause skin damage. Chronic exposure to UV irradiation causes decreased collagen synthesis, degenerative changes in collagen bundles, accumulation of elastotic material and increased epidermal thickness. Origanum hypericifolium, an endemic Turkish plant, belongs to Lamiaceae family. The main constituents of its oil are monoterpenes including cymene, carvacrol, thymol and γ-terpinene. The effects of undiluted O. hypericifolium oil on UVB irradiated skin of mice were investigated histochemically. Four groups of female BALB/c mice, whose dorsal hair was shaved, were allocated as follows: non-UVB irradiated (Group 1), UVB-irradiated (Group 2), O. hypericifolium oil treated (Group 3), and O. hypericifolium oil treated and UVB irradiated (Group 4). Sections of dorsal skin samples were stained with Mallory's phosphotungstic acid hematoxylin for collagen fibers and Taenzer-Unna orcein for elastic fibers. Sections also were stained with hematoxylin and eosin to measure epidermal thickness. We observed intense staining of collagen and homogeneous, scattered thin elastic fibers in Group 1; scattered and weakly stained collagen and curled, amorphous, accumulate elastic fibers in Group 2; and intense staining of collagen in Groups 3 and 4. Accumulation of elastic fibers in the dermis was unremarkable in Groups 3 and 4. In Groups 3 and 4, O. hypericifolium oil treatment thickened the epidermis. Epidermal thickness was greatest in Group 4. We suggest that O. hypericifolium oil may block UVB induced alterations of collagen and elastic fibers, and increase epidermal thickness.

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

“…The mixture of primrose oils was found to inhibit UV-induced wrinkle formation via the regulations of MMP, MAP kinase, AP-1 transcription factor, and TGF-β2 [5], and the essential oils of Cnidum officinale makino and Ligusticum chuanxiong hort significantly inhibited UV-induced DNA damage and apoptosis in NIH 3T3 cells [6]. In addition, the topical application of almond oil reduced UV-induced histopathological alterations in mice skin [7], and patchouli and Origanum hypericifolium oils were reported to suppress UV-induced wrinkle formation, skin elasticity loss, and collagen and epidermal thickness increases in mice [8,9]. Furthermore, in hairless mice, Angelica pubescens oil improved the condition of UV-induced damaged skins [10].…”

Carvacrol attenuates amikacin-induced nephrotoxicity in the rat

“…CAR is a monoterpenic phenol found in the essential oil of Labitae, including Origanum, Satureja, Thymbra, Thymus, and Corydothymus (Ili and Keskin 2013;Shahrokhi Raeini et al 2020). CAR has lipophilic properties and numerous biological and pharmacological activities, including antioxidant, anticancer, antibacterial, antifungal, hepatoprotective both in vitro and in vivo ( Fig.…”

“…In recent years, numerous studies have been conducted to nd effective renoprotective compounds that can be used in clinical practice (Rehman et al 2014). CAR is a monoterpenic phenol found in the essential oil of Labitae, including Origanum, Satureja, Thymbra, Thymus, and Corydothymus (Ili and Keskin 2013;Melo et al 2011). It is widely used as an additive in the food industry (Ultee et al 1999).…”

“…It is widely used as an additive in the food industry (Ultee et al 1999). CAR has numerous biological and pharmacological activities, including antioxidant, anticancer, antibacterial, antifungal, hepatoprotective both in vitro and in vivo (Ili and Keskin 2013;Potočnjak and Domitrović 2016;Suntres et al 2015). CAR has an anti-in ammatory effect by increasing the synthesis of IL-10 and decreasing the production of proin ammatory mediators such as IL-1β (da Silva Lima et al 2013).…”