A histochemical and quantitative study of phosphatase in the placenta and fetal membranes of the guinea pig

Hard, Walter L.

doi:10.1002/aja.1000780103

Cited by 40 publications

(1 citation statement)

References 19 publications

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“…In a preliminary report of his studies, Corner (1944) suggested that in tissues in which it was not associated with the storage of inorganic phosphate as in bone, the enzyme may be involved in lipid metabolism. The same suggestion was made by Emmel (1945) and Hard (1946) in their studies in small intestine and placenta, respectively.…”

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confidence: 67%

Alkaline Phosphatase Activity in the Theca of Ovarian Follicles of the Hen Throughout Follicular Development

et al. 1996

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Within the hen's ovary, the theca tissue of small follicles that have not yet entered the preovulatory hierarchy is a major contributor to ovarian steroidogenesis. Relatively little is known about developmental stages in the theca layer within this pool of follicles and of the histological properties of this tissue. In pigs and sheep, alkaline phosphatase activity has been identified in the theca of preovulatory follicles and in theca-derived cells in the corpus luteum. The objectives of this study were to document morphological changes in the theca layer through pre- and postovulatory follicular development, and to assess alkaline phosphatase activity at these developmental stages. Ovarian tissue containing small white, large white, small yellow, large yellow, and postovulatory follicles (POF) was obtained from mature White Leghorn hens and embedded in either methacrylate for morphological assessment or paraffin for alkaline phosphatase histochemistry. Alkaline phosphatase activity was found to be specific for cells of the theca interna in preovulatory follicles. Activity was first detectable in very small white follicles, the earliest stage in which the theca interna could be distinguished from the theca externa, and highest in the yellow yolky follicles. After ovulation, activity decreased to undetectable levels by the POF4 to 5 stages in POF within 4 to 5 d after ovulation. This study has shown that in the domestic hen, alkaline phosphatase activity is present in cells adjacent to the basal lamina of the theca interna of preovulatory follicles and POF up to 4 to 5 d after ovulation. Identification of the functional significance of this histological reaction will provide new information on ovarian function in the hen.

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Section: Discussionsupporting

confidence: 67%

Alkaline Phosphatase Activity in the Theca of Ovarian Follicles of the Hen Throughout Follicular Development

et al. 1996

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The histochemistry of the rodent's placenta

1946

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Further observations on the distribution of phosphatases in mammalian placentas

Dempsey

Wislocki

1947

Am. J. Anat.

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TWENTY-FOUR FIGURESDuring the last few years we have investigated the occurrence and distribution of a number of substances in mammalian placentas, using a variety of cytochemical procedures. Previous papers from this laboratory have dealt with various aspects of the human placenta (Dempsey and Wislocki, '44, '45; Wislocki and Dempsey, '45, '46a) as well as with the placentas of cats (IVislocki and Dempsey, '46b), pigs (Wislocki and Dempsey, '46c) and rodents (Wislocki, Deane and Dempsey, '46). The procedures f o r demonstrating histochemically the distribution of acid and alkaline glycerophosphatases (Comori, '41a, '41b) have figured prominently in these investigations. Since completing the studies listed above, we have found that histochemically demonstrable phosphatases can react with several phosphorylated substrates other than glycerophosphate (Dempsey and Deane, '46), and that the histological localization characteristic for one substrate may differ from that observed when another phosphate is used. This information has prompted us to re-examine the localization of phosphatases in mammalian placentas, using several substrates rather than the single one previously employed. We have also had occasion to apply

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A histochemical and quantitative study of phosphatase in the placenta and fetal membranes of the guinea pig

Cited by 40 publications

References 19 publications

Alkaline Phosphatase Activity in the Theca of Ovarian Follicles of the Hen Throughout Follicular Development

Alkaline Phosphatase Activity in the Theca of Ovarian Follicles of the Hen Throughout Follicular Development

The histochemistry of the rodent's placenta

Further observations on the distribution of phosphatases in mammalian placentas

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