A highly stable, sensitive, regenerable and rapid immunoassay for detecting aflatoxin B1 in corn incorporating covalent AFB1 immobilization and a recombinant Fab antibody

Rapid analysis of aflatoxins B1, B2, and ochratoxin A in rice samples using dispersive liquid-liquid microextraction combined with HPLC

“…The accurate and sensitive determination of mycotoxins is currently an important requirement to meet food safety concerns and new official legislated regulations. Different analytical techniques have been used for the determination of mycotoxins in cereals or cereal products, including HPLC with fluorescence detection (FLD) , GC–MS , and ELISA . In recent years, research has focused on methods capable of measuring multiple mycotoxins in a single analytical procedure.…”

Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection

“…These are mainly based on solutions at low pH (HCl, Glycine-HCl), or at high pH (NaOH) or at high ionic strength (MgCl2) [ 18 ]. From five up to 39 regeneration cycles are reported for the whole antibody molecule [ 18 , 19 ], while for Fab′ molecules, a regeneration of four cycles has been found [ 20 ]. Different solutions were tested on our Fab′-based platform.…”

Métodos imunológicos para a detecção de fungos toxigênicos e micotoxinas em grãos, alimentos e rações

“…Em cereais, este limite é de 5,0 µg/Kg; em castanha-do-pará varia de 10,0 µg/Kg a 20,0 µg/Kg; e em amendoim e em milho, incluindo farinha e semolina, é de 20,0 µg/Kg (Tabela 24.1). Diversos métodos imunológicos podem ser utilizados para detecção de aflatoxinas, incluindo o ELISA 17,[113][114][115][116][117][118][119][120][121] , imunocromatografia 118,[121][122][123][124][125][126] , dipstick 30,127 e biossensores 128-134 .…”