A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections

Lu, Chi-Jen; Chang, Luan‐Yin; Chen, Pei‐Jer; Xia, Ningshao; Shao, Pei-Lan; Huang, Li‐Min

doi:10.1016/j.jfma.2011.11.029

Cited by 13 publications

(10 citation statements)

References 12 publications

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“…New tests or techniques for specific microorganisms may help us find the etiology of KD. 48,49 In conclusion, we found that common respiratory viruses, such as enteroviruses, adenoviruses, rhinoviruses, and coronaviruses, were associated with KD. Heterogeneous infectious etiologies may be responsible for KD in different countries as well as during different seasons.…”

Section: Discussionmentioning

confidence: 65%

Viral infections associated with Kawasaki disease

Chang

Shao

et al. 2014

Journal of the Formosan Medical Association

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Section: Discussionmentioning

confidence: 65%

Viral infections associated with Kawasaki disease

Chang

Shao

et al. 2014

Journal of the Formosan Medical Association

Self Cite

206

182

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show abstract

“…ELISA sensitivity decreases after 10 5 (log 10/ml) viral load but QuickVue Influenza A+B test sensitivity decreases after 10 7 (log 10/ml) viral load [51]. In a different study, 1086 sera were analysed from 43 swine herds for different reference strains (H1N1, H3N2, H1N2, H1N1pdm) using ELISA with HI test and it was found that ELISA sensitivity and specificity were higher than HI that were 72.65% and 63.01%, respectively, for the detection of these strains [52,53]. According to these studies, ELISA cannot detect infection at an early stage.…”

Section: Enzyme Linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

et al. 2020

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H1N1 (Swine flu) is caused by influenza A virus, which is a member of Orthomyxoviridae family. Transmission of H1N1 occurs from human to human through air or sometimes from pigs to humans. The influenza virus has different RNA segments, which can reassert to make new virus strain with the possibility to create an outbreak in unimmunized people. Gene reassortment is a process through which new strains are emerging in pigs, as it has specific receptors for both human influenza and avian influenza viruses. H1N1 binds specifically with an α-2,6 glycosidic bond, which is present in human respiratory tract cells as well as in pigs. Considering the fact of fast multiplication of viruses inside the living cells, rapid detection methods need an hour. Currently, WHO recommended methods for the detection of swine flu include real-time PCR in specific testing centres that take 3–4 h. More recently, a number of methods such as Antigen–Antibody or RT-LAMP and DNA biosensors have also been developed that are rapid and more sensitive. This review describes the various challenges in the diagnosis of H1N1, and merits and demerits of conventional vis-à-vis latest methods with special emphasis on biosensors.

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“…Rapid techniques for detecting the influenza virus include immunofluorescence and immunoenzymatic assays [ [66] , [67] , [68] ]. For both viruses, real-time RT-PCR represents the gold standard technique [ 69 ]. Rapid immunochromatographic tests will be extremely important for mass screening of individuals in the COVID-19 pandemic [ 70 , 71 ].…”

Section: A Brief Comparison Between Clinical Aspects and Laboratory Dmentioning

confidence: 99%

Comparative epidemiology between the 2009 H1N1 influenza and COVID-19 pandemics

Costa

Saivish

Santos

et al. 2020

Journal of Infection and Public Health

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In a short time, humanity has experienced two pandemics: the influenza A virus pandemic (pH1N1) in 2009 and the coronavirus disease 2019 (COVID-19) pandemic in 2020. Therefore, it is likely that the general population will erroneously seek to compare the two pandemics and adopt similar attitudes in facing them. However, the two pandemics have their intrinsic characteristics that distinguish them considerably; for example, the virulence of the infectious agents and the availability of treatment and vaccine. Consequently, given this knowledge gap between the pH1N1 and COVID-19 pandemics, we conducted this review to clarify and summarize, above all, the epidemiological historical aspects of these two viruses of great importance to global public health.

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A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections

Abstract: The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity.

Cited by 13 publications

References 12 publications

Viral infections associated with Kawasaki disease

Viral infections associated with Kawasaki disease

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

Comparative epidemiology between the 2009 H1N1 influenza and COVID-19 pandemics

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