A highly specific and sensitive ratiometric fluorescent probe for carbon monoxide and its bioimaging applications

Abstract

A simple highly specific and sensitive ratiometric fluorescent probe has been constructed to monitor carbon monoxide levels in living cells.

An Easily Available Ratiometric Reaction-Based AIE Probe for Carbon Monoxide Light-up Imaging

“…The detection limit of CO was thus calculated to be 30.8 nM. All these results verified that ratiometric detection gave a better performance, consistent with our initial design and previous reports. − …”

“…Fluorescent probes − have aroused great interest because of their high specificity and sensitivity, simple operation, noninvasive detection, and imaging capability. − Pioneered by the work of He’s group and Chang’s group, several fluorescent CO probes have been reported (Table S1). − However, most of the current probes suffered from the notorious aggregation-caused quenching (ACQ) effect after they accumulated in cells, making the fluorescence emission much weaker than that in solution . Thus, it is highly desirable to develop anti-ACQ fluorescent probes for CO detection and imaging.…”

“…Furthermore, we examined the response of BTCV-CO (5 μM) in PBS buffer (pH 7.4, containing 5% DMSO) toward CO. A water-soluble CO-releasing molecule, [Ru­(CO) 3 Cl 2 ] 2 (CORM-2), was used as the CO donor. − As shown in Figure S10B, BTCV-CO displayed weak emission in PBS solution. The addition of PdCl 2 (5 μM) has negligible influence on the PL spectra of BTCV-CO.…”

Recent Advances in Fluorescence Light‐Up Endogenous and Exogenous Carbon Monoxide Detection in Biology

“…The allylic ether moiety was removed by the Pd(0) mediated Tsuji-Trost reaction, the stronger ICT process would be recovered and the fluorophore was released, leading to an obvious redshift in the fluorescence emission spectrum, supplemented by cell images. [56] While, a similar phenomenon was reported by Qi et al with a NIR active and colorimetric probe 1, having allyl carbonate as the signalling unit ( Figure 16). It has also been applied to get cell image in MCF-7 cells due to its bright fluorescence.…”

“…reported a 4‐hydroxynaphthalimide based probe Ratio‐CO (Figure ) having allyl ether as the reaction site. The allylic ether moiety was removed by the Pd(0) mediated Tsuji‐Trost reaction, the stronger ICT process would be recovered and the fluorophore was released, leading to an obvious red‐shift in the fluorescence emission spectrum, supplemented by cell images . While, a similar phenomenon was reported by Qi et al.…”