An Easily Available Ratiometric Reaction-Based AIE Probe for Carbon Monoxide Light-up Imaging

Abstract

Carbon monoxide (CO) is a significant gasotransmitter that naturally modulates inflammatory responses. Visualization of CO in situ would help to reveal its physiological/pathological functions. Unfortunately, most existing CO fluorescent probes show aggregation-caused quenching (ACQ) properties. Herein, we report the reaction-based fluorescent probe (BTCV-CO) with aggregation-induced emission (AIE) characteristics for CO detection and imaging. This ratiometric AIE probe showed excellent stability, high sensitivity (detection limit of 30.8 nM), and superior selectivity. More importantly, this CO-responsive AIE probe could be facilely designed and easily obtained by two-step synthesis with high yield, providing an easy-to-handle AIE toolbox for real-time visualization of CO in a living system.

Fluorescent small organic probes for biosensing

“…14 In 2019, Tang et al published an AIE probe for imaging carbon monoxide (CO). 99 Whilst known for its toxicity, CO is also an endogenous gasotransmitter and is involved in a range of disease states and biological pathways. The probe, BTCV-CO, was designed to undergo a Tsuji-Trost reaction in the presence of CO and Pd 2+ (Scheme 25).…”

Recent Advances in Fluorescence Light‐Up Endogenous and Exogenous Carbon Monoxide Detection in Biology

“…The work is unique in providing a new approach to produce reaction based easily accessible AIE probe to monitor the time course of CO visualization for the biological/pathological purpose. [64] Klán and co-workers presented a series of palladacycle based on Nile red derivative, judiciously chosen for improving the sensitivity and water solubility in comparison to previous reports. The palladium centre reacts with CO to make free the highly emissive Nile red compound to detect this small molecule.…”

“…This selective light‐up procedure was efficiently introduced in the in vitro cell imaging showing intense green fluorescence (Figure ) and also in vivo detection of CO in living mice with time dependent fluorescence enhancement. The work is unique in providing a new approach to produce reaction based easily accessible AIE probe to monitor the time course of CO visualization for the biological/pathological purpose …”

Twisted intramolecular motion arrested in aggregated state emission and the nonlinear optical properties of pyrene pyrazoline derivatives

“…Fluorescent organic small molecules have received significant attention in modern technology for their vital application in various research areas such as organic light-emitting diodes, [1] organic lasers, [2] organic field effect transistors, [3] organic dye solar cells, [4] organic ligands for detection of hazardous metal ions, [5,6] fluorescent probes [7] and bio-imaging. [8,9] Conventional organic luminophores have drawbacks of molecular aggregation and strong intermolecular or intramolecular interactions; emission losses in solution or in the solid state along with low intensity of emission are known as aggregation-caused quenching (ACQ). [10] These types of molecules are not effectively used and have difficulties for practical application.…”