A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus

Jung, Il Young; You, Jae Bem; Choi, Bo Ram; Kim, Ji Su; Lee, Hyun Kyung; Jang, Bora; Jeong, Han Saem; Lee, Kyuri; Im, Sung Gap; Lee, Hyukjin

doi:10.1002/adhm.201600334

Cited by 41 publications

(45 citation statements)

References 23 publications

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“…At the optimized concentration of antibodies (10 μg.mL −1 ), we changed the incubation time at different points (5,10,15,20,30,40, and 60 min) and the SWV measurements were recorded in 5 mM [Fe (CN) 6 ] 3−/4solution.…”

Section: Binding Timementioning

confidence: 99%

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An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

2019

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The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL −1 and 0.01 to 10,000 ng.mL −1 were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL −1 for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples.

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Section: Binding Timementioning

confidence: 99%

“…The LOD of this assay was 10 times more sensitive than rRT-PCR method [19]. Another new generation system was developed for the robust and facile diagnosis of MERS-CoV based on isothermal rolling circle amplification (RCA) method [20]. However, these methods are still time consuming and costly which limits their wide applications.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

2019

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“…For instance, Jung et al described a platform in which the introduction of short DNA primers complementary to the DNA products, initiated a DNA polymerization reaction that resulted in a quick shrinkage of the hydrogel construction. By means of this assay, a robust and fast diagnosis of Middle East respiratory syndrome coronavirus (MERS-CoV) became possible with a 100-fold enhancement in sensitivity after 30 min of reaction time [82]. More recently, Na and coworkers presented a system in which the primers were immobilized on microbeads instead of channel surface immobilization ( Fig.…”

Section: Dna Hydrogels For Point-of-care (Poc) Applicationsmentioning

confidence: 99%

“…Schematic of the detection of pathogenic viruses driven by DNA hydrogel formation through RCA of primer immobilized microbeads. Adapted from ref [82]…”

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confidence: 99%

DNA hydrogel-empowered biosensing

Khajouei

Ravan

Ebrahimi

2020

Advances in Colloid and Interface Science

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DNA hydrogels as special members in the DNA nanotechnology have provided crucial prerequisites to create innovative gels owing to their sufficient stability, biocompatibility, biodegradability, and tunable multifunctionality. These properties have tailored DNA hydrogels for various applications in drug delivery, tissue engineering, sensors, and cancer therapy. Recently, DNA-based materials have attracted substantial consideration for the exploration of smart hydrogels, in which their properties can change in response to chemical or physical stimuli. In other words, these gels can undergo switchable gel-to-sol or sol-to-gel transitions upon application of different triggers. Moreover, various functional motifs like i-motif structures, antisense DNAs, DNAzymes, and aptamers can be inserted into the polymer network to offer a molecular recognition capability to the complex. In this manuscript, a comprehensive discussion will be endowed with the recognition capability of different kinds of DNA hydrogels and the alternation in physicochemical behaviors upon target introducing. Finally, we offer a vision into the future landscape of DNA based hydrogels in sensing applications.

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“…Our group previously reported a robust molecular diagnostic platform named “DhITACT: DNA hydrogel formation by isothermal amplification of complementary targets.” We have successfully incorporated this DhITACT system into microfluidic channel for optical detection of infectious viruses such as Ebola and MERS. In the presence of target mRNA, DNA hydrogel can be formed by physical entanglement of amplified DNA products.…”

mentioning

confidence: 99%

Capillary Tube Based Molecular Diagnostic Test for Naked Eye Detection of Antibiotic Resistant Bacteria

Choi

Cho

Kim

et al. 2018

Adv Materials Technologies

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Antibiotic resistance bacteria (ARB) are a rising threat to public health. To prevent the emergence of antibiotic resistance strains of bacteria, selectively prescribing antibiotics is of the utmost importance. Under infection control in hospitals, rapid and accurate diagnosis of antibiotic resistant bacteria is highly critical. This study shows a new diagnostic platform for naked‐eye detection of antibiotics resistant bacteria using a capillary tube based optical detection method. For specific detection of target ARB, DNA hydrogel formation by isothermal amplification of complementary target (DhITACT) system is incorporated with magnetic beads (MBs). To facilitate naked‐eye optical detection, a molecular probe is immobilized on the surface of MBs. After selectively capturing target to the probe, generation of long DNA strands is achieved through isothermal rolling circle amplification (RCA). Physical entanglement of amplified DNA products triggers rapid interparticle aggregation among MBs. Using this phenomenon, a capillary tube‐based naked eye detection test is developed. This system offers facile optical detection of ARB with minimal procedures to support its wide use in a clinical set‐up.

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A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus

Cited by 41 publications

References 23 publications

An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

DNA hydrogel-empowered biosensing

Capillary Tube Based Molecular Diagnostic Test for Naked Eye Detection of Antibiotic Resistant Bacteria

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