A highly sensitive long-wavelength fluorescence probe for nitroreductase and hypoxia: selective detection and quantification

Guo, Ting; Cui, Lei; Shen, Jiaoning; Zhu, Weiping; Xu, Yufang; Qian, Xuhong

doi:10.1039/c3cc45367g

Cited by 124 publications

(63 citation statements)

References 43 publications

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“…The detection is based on the selective reduction of the nitro group to a hydroxylamine or amino group by nitroreductase in the presence of NADPH as an electron donor. It was demonstrated by confocal fluorescence imaging that CyNNO 2 could detect intracellular hypoxia in A549 tumor cells incubated with CyNNO 2 by imaging nitroreductase by dual emission ratiometry, which showed emission channels of 700–770 nm and 780–800 nm on excitation at 635 nm [42]. A “turn-on fluorescent” NIR probe AZO-DCM (13a) was designed for selective and sensitive in vivo detection of cytochrome P450 reductase which is overexpressed in hypoxic tumor cells.…”

Section: Small Molecule and Dye Based Probesmentioning

confidence: 99%

Luminescent Probe Based Techniques for Hypoxia Imaging

Jaworski¹

2017

JNMR

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Hypoxia is a condition of tissue environments wherein a lower than normal level of oxygen is available, and it serves as the root cause and indicator of various diseases. Detection of hypoxia in tumors is imperative for furthering our understanding of the pathological effects and the development of proper treatments, as it is well established that hypoxic tumors are able to impede the cancer treatment process by being resistant to many therapies. It is important therefore to be able to detect hypoxia in tissues and tumors through in vivo imaging methods. A growing area for detection of hypoxia in vivo is the use of fluorescent/luminescent probes which has accelerated in recent years. The continued quest for improvements in selectivity and sensitivity has inspired researchers to pursue new strategies for fluorescence/luminescent probe design. This review will discuss various luminescent probes based on small molecules, dyes, macromolecules, and nanoparticles for sensitive and specific detection of oxygen levels directly or by indirect mechanisms such as the presence of enzymes or related factors that arise in a hypoxic environment. Following the particular mechanism of detection, each probe has specific structural and photophysical properties which permit its selectivity and sensitivity. These probes show promise in terms of low toxicity and high specificity among other merits discussed, and in providing new dimensions for hypoxia detection, these works contribute to future potential methods for clinical diagnosis of hypoxic tissues and tumors.

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Section: Small Molecule and Dye Based Probesmentioning

confidence: 99%

Luminescent Probe Based Techniques for Hypoxia Imaging

Jaworski¹

2017

JNMR

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“…These materials have the advantages of a minimal background signal due to low autoabsorption and autofluorescence low-light scattering and deep light penetration into tissues. Until now, long-wavelength luminescent organic dye (Chen et al, 1999;Umezawa et al, 2009;Guo et al, 2013) and nanoparticles (Tian et al, 2012) have been commonly used as labeling probes and have been widely applied for chemical and biological labeling, sensing, and bioimaging. As an alternative to dye molecules and nanoparticles, lanthanide (Ln)-doped upconversion nanoparticles (Cheng et al, 2012;Ding et al, 2013;Tu and Wang 2013) are considered to be a promising new class of long-wavelength luminescent materials.…”

Section: Introductionmentioning

confidence: 99%

Luminescence energy transfer detection of PSA in red region based on Mn2+-enhanced NaYF4:Yb, Er upconversion nanorods

Zhang

Wang

Gao

et al. 2015

Biosensors and Bioelectronics

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“…There are examples in the literature for fluorogenic phenoxazines. In most of these reports, the fluorogenicity is based either on the polarity‐sensitive nature of the fluorescent core or on the enzymatic removal of N‐acyl substituents –. Recently, an azide‐quenched benzophenoxazine was also reported .…”

Section: Introductionmentioning

confidence: 99%

New Red‐Emitting Tetrazine‐Phenoxazine Fluorogenic Labels for Live‐Cell Intracellular Bioorthogonal Labeling Schemes

Knorr

Kozma

Herner

et al. 2016

Chemistry A European J

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The synthesis of a set of tetrazine-bearing fluorogenic dyes suitable for intracellular labeling of proteins in live cells is presented. The red excitability and emission properties ensure minimal autofluorescence, while through-bond energy-transfer-based fluorogenicity reduces nonspecific background fluorescence of unreacted dyes. The tetrazine motif efficiently quenches fluorescence of the phenoxazine core, which can be selectively turned on chemically upon bioorthogonal inverse-electron-demand Diels-Alder reaction with proteins modified genetically with strained trans-cyclooctenes.

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A highly sensitive long-wavelength fluorescence probe for nitroreductase and hypoxia: selective detection and quantification

Cited by 124 publications

References 43 publications

Luminescent Probe Based Techniques for Hypoxia Imaging

Luminescent Probe Based Techniques for Hypoxia Imaging

Luminescence energy transfer detection of PSA in red region based on Mn2+-enhanced NaYF4:Yb, Er upconversion nanorods

New Red‐Emitting Tetrazine‐Phenoxazine Fluorogenic Labels for Live‐Cell Intracellular Bioorthogonal Labeling Schemes

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