A highly sensitive and stable glucose biosensor using thymine-based polycations into laponite hydrogel films

Zanini, Verónica I. Paz; Gavilán, Maximiliano; Mishima, B. A. López de; Martino, Debora Marcela; Borsarelli, Claudio D.

doi:10.1016/j.talanta.2015.12.046

Cited by 21 publications

(22 citation statements)

References 46 publications

(54 reference statements)

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“…Pza Zanini et al prepared glucose bioelectrodes by glucose oxidase immobilization into laponite hydrogel films containing DNA bioinspired polycations. Results indicated that the electrochemical properties of the laponite hydrogel films were largely improved by the incorporation of thymine‐based polycations, and are even proportional to the positive charge density of the polycation molecule . A biosensor matrix based on UV‐initiated graft copolymerized poly (ethylene glycol) methacrylate and 2‐hydroxyethyl methacrylate has been studied using imaging surface plasmon resonance.…”

Section: Applications Of Gradient Stiffness Hydrogels In Vitromentioning

confidence: 99%

“…Results indicated that the electrochemical properties of the laponite hydrogel films were largely improved by the incorporation of thymine-based polycations, and are even proportional to the positive charge density of the polycation molecule. 107 A biosensor matrix based on UV-initiated graft copolymerized poly (ethylene glycol) methacrylate and 2-hydroxyethyl methacrylate has been studied using imaging surface plasmon resonance. The performance of the hydrogel matrix was studied through an in situ biomolecular interaction study of human serum albumin (HSA).…”

Section: Hydrogel-based Biosensorsmentioning

confidence: 99%

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A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine

Xia

Liu

Yang

2017

J Biomedical Materials Res

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Substrate stiffness is known to impact characteristics including cell differentiation, proliferation, migration and apoptosis. Hydrogels are polymeric materials distinguished by high water content and diverse physical properties. Gradient stiffness hydrogels are designed by the need to develop biologically friendly materials as extracellular matrix (ECM) alternatives to replace the separated and narrow-ranged hydrogel substrates. Important new discoveries in cell behaviors have been realized with model gradient stiffness hydrogel systems from the two-dimensional (2D) to three-dimensional (3D) scale. Basic and clinical applications for gradient stiffness hydrogels in tissue engineering and regenerative medicine continue to drive the development of stiffness and structure varied hydrogels. Given the importance of gradient stiffness hydrogels in basic research and biomedical applications, there is a clear need for systems for gradient stiffness hydrogel design strategies and their applications. This review will highlight past work in the field of gradient stiffness hydrogels fabrication methods, mechanical property test, applications as well as areas for future study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1799-1812, 2017.

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Section: Applications Of Gradient Stiffness Hydrogels In Vitromentioning

confidence: 99%

Section: Hydrogel-based Biosensorsmentioning

confidence: 99%

A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine

Xia

Liu

Yang

2017

J Biomedical Materials Res

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“…Plot of oxidation and reduction current versus square root of the scan rate showed a linear relationship based on Randles-Sevcik equation, i p = (2.69 × 10 5 )n 3/2 AcD 1/2 v 1/2 where i p , n, A, c, D and v refer to peak current (A), number of electrons react in the reaction (1 for K 3 [Fe(CN) 6 ]), electrochemically effective surface area of the working electrode (cm 2 ), concentration of the reactant (mol cm -3 ), diffusion coefficient (7.6 × 10 -6 cm 2 s -1 for K 3 [Fe(CN) 6 ) and scan rate (V s -1 ). The linear relationship between redox current and the square root of the scan rate indicates that the process on the surface of the electrode was diffusioncontrolled (Dai et al 2013;Othman et al 2016;Paz Zanini et al 2016) (Figure 7(c)). Furthermore, the increasing of peak separation from oxidation to reduction potential with the increasing of scan rate shows that the system is quasi-reversible (Tyagi et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Surface Modification of Cellulose Nanomaterial for Urea Biosensor Application

Khalid¹,

Lee²,

Arip³

2018

JSM

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Cellulose nanomaterial with rod-like structure and highly crystalline order, usually formed by elimination of the amorphous region from cellulose during acid hydrolysis. Cellulose nanomaterial with the property of biocompatibility and nontoxicity can be used for enzyme immobilization. In this work, urease enzyme was used as a model enzyme to study the surface modification of cellulose nanomaterial and its potential for biosensor application. The cellulose nanocrystal (CNC) surface was modified using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation to introduce the carboxyl group at C6 primary alcohol. The success of enzyme immobilization and surface modification was confirmed using chemical tests and measured using UV-Visible spectrophotometer. The immobilization strategy was then applied for biosensor application for urea detection. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electroanalytical characterization of the urea biosensor.

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“…Thus, VBT is often polymerized in different ratios with styrene derivative charged monomers, such as anionic vinyl phenyl sulfonate (VPS) salts [7] or cationic vinylbenzyl triethylammonium chloride (VBA) [8][9][10] which allows the copolymer to be soluble in water. Extending the knowledge achieved from the study of the natural phenomena related to thymine reactivity and intermolecular interactions has generated a diapason of potential applications [11][12][13][14] ranging from photoresist material [15,16] and organic-inorganic hybrid devices [2] to antibacterial coatings [17], drug delivery systems [3,[18][19][20], recyclable plastics [21], and biosensors [22,23]. On the other hand, the increasing attention given to the environmental and toxicological dilemmas linked to commercial materials situates the thymine-based polymeric systems as a Green Chemistry platform [24].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of 1,3-Bis(vinylbenzyl)thymine: Copolymerization with Vinylbenzyl Thymine Ammonium Chloride

Chen

Martino³

et al. 2017

Journal of Polymers

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A novel bioinspired molecule, 1,3-bis(vinylbenzyl)thymine (bisVBT), was isolated as a by-product during the synthesis of 1-(4-vinylbenzyl)thymine (VBT) and analyzed with various techniques: NMR, IR, and Single-Crystal X-ray Diffraction. In addition to embodying all the desired characteristics of VBT (i.e., having the ability to undergo a 2 + 2 photodimerization reaction upon UV irradiation, a derivatization site, hydrogen bonding sites, and aromatic stacking ability) the bisVBT monomer has the added benefit of having two vinyl groups for cross-polymerization. Copolymerizing the bisVBT monomer with the charged monomer vinylbenzyl triethylammonium (VBA) chloride, different copolymers/terpolymers/cross-linked network were obtained, as it was shown by the absence of the vinyl resonance in the NMR spectra. Thermal Gravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) showed an indication of materials with low degree of cross-linking. A Gel Permeation Chromatography (GPC) method was improved to better characterize the molecular weight distributions of the cationic structures. Preliminary qualitative cross-linking studies were performed on bisVBT-VBA copolymers, and a comparison with VBT-VBA copolymers is presented.

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A highly sensitive and stable glucose biosensor using thymine-based polycations into laponite hydrogel films

Cited by 21 publications

References 46 publications

A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine

A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine

Surface Modification of Cellulose Nanomaterial for Urea Biosensor Application

Isolation and Characterization of 1,3-Bis(vinylbenzyl)thymine: Copolymerization with Vinylbenzyl Thymine Ammonium Chloride

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