A highly sensitive and dual-readout immunoassay for norfloxacin in milk based on QDs-FM@ALP-SA and click chemistry

Liu, Xiao; Xu, Zehua; Han, Zhenyu; Fan, Longxing; Li, Sha; Han, Yang; Chen, Zongfen; Sun, Tieqiang; Ning, Baoan

doi:10.1016/j.talanta.2021.122703

Cited by 16 publications

(7 citation statements)

References 37 publications

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“…The IC50 of the developed fluorescence ELISA was 2.43 μg L -1 , and the IC15 of the developed fluorescent ELISA was 0.20 μg L -1 , which was about 12-fold lower than the IC15 of the conventional ELISA (2.50 μg L -1 ), with an IC50 of 21.98 μg L -1 . As shown in Table 1, the analytical feature of our established method was compared to several immunoassays for NOR determination (Cheng et al, 2022;Han et al, 2021;Han et al, 2022;Hu, Gao, Han, & Yang, 2020;Jin et al, 2022;Liu et al, 2021;Wu et al, 2017;Yu et al, 2018;Zong et al, 2019). The developed method showed ideal LOD and detection time.…”

Section: Construction Of Qds-based Fluorescent Quenching Elisamentioning

confidence: 99%

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

et al. 2023

Preprint

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A highly sensitive and reliable fluorescence immunoassay based on the inner-filter effects (IFE) of quantum dots (QDs) was developed for the sensitive detection of norfloxacin (NOR) in animal-origin foods. Briefly, the enzymatic formation of horseradish peroxidase in conventional ELISA effectively quenched the fluorescence of QDs through IFE, and then the absorption signal of conventional ELISA was transformed to fluorescent signals, resulting in a higher sensitivity for NOR detection in animal-origin foods.The detection limit of NOR in this developed method was 0.20 µg L− 1 in standard solution, which was 12-fold more sensitive than conventional ELISA (2.50 µg L− 1). The results of specificity analysis revealed that the proposed method could not only be used for the detection of NOR, but also for the other 9 structural analogues. The recovery rates of NOR-spiked animal-origin foods ranged from 88.11–108.16%, with CV values of 1.95%-18.03%. In addition, the established method had good consistency with the commercial ELISA kits and HPLC, indicating the accuracy of the developed method for the detection of NOR in real samples. In conclusion, the established fluorescence immunoassay was sensitive and facile, and could be alternatively used for the determination of NOR residues in animal-origin foods.

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Section: Construction Of Qds-based Fluorescent Quenching Elisamentioning

confidence: 99%

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

et al. 2023

Preprint

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“…Fluoroquinolones (FQs) comprise a group of chemical synthetic antibiotics with efficient antibacterial properties against both Gram-negative and Gram-positive bacteria, blocking bacterial DNA replication by inhibiting the activity of topoisomerase IV and DNA gyrase. 1–3 In recent decades, although FQs have played a crucial role in the treatment of various diseases in humans and animals, 4 the abuse of FQs for the purpose of growth promotion and disease prevention has posed great risk to the ecological environment and human health. 5 Some farmers have been nonchalant concerning the over-dose and over-range use of FQs in pursuit of economic benefits, which has resulted in the presence of drug residues in animal-origin food because they are incompletely metabolized in a short period of time.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent strip sensor for rapid and ultrasensitive determination of fluoroquinolones in fish and milk

Lei

Guo

et al. 2023

Analyst

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“…25 Besides, FAs such as lomefloxacin, ofloxacin, enrofloxacin, norfloxacin, and ciprofloxacin may coexist in the environment making detection more difficult. Nowadays, various techniques have been applied for FA detection, such as high-performance liquid chromatography, 28 immunoassays, 29 capillary electrophoresis, 26 and enzymelinked immunosorbent assays. 30 Nevertheless, some drawbacks including complicated pretreatment and being time-consuming and expensive limit the application of these methods.…”

Section: Introductionmentioning

confidence: 99%

A Direct Z-Scheme AgBr/CuBi₂O₄ Photocathode for Ultrasensitive Detection of Ciprofloxacin and Ofloxacin by Controlling the Release of Luminol in Self-Powered Microfluidic Photoelectrochemical Aptasensors

Dai

et al. 2022

Anal. Chem.

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An innovative self-powered microfluidic photoelectrochemical (PEC) aptasensor was developed that uses photoactive AgBr/CuBi2O4 (ACO) composites as the photocathode matrix for ultrasensitive detection of ciprofloxacin (CIP) and ofloxacin (OFL). The formation of direct Z-scheme heterojunctions in ACO composites greatly aided electron/hole pair separation. Meanwhile, ZnIn2S4-decorated CdS nanorod arrays (CZIS) as the photoanode were used instead of a platinum counter electrode to provide electrons. The “signal-off” CIP detection was accomplished through the steric hindrance effect in the photoanode due to the combination of aptamer(CIP) and CIP. To increase the cathodic photocurrent intensity for OFL determination, controlled release of luminol was first used. Luminol molecules were successfully embedded in the porous structure of silicon dioxide nanospheres (PSiO2) by the electrostatic adsorption between PSiO2 and aptamer(OFL). The luminol released by specific recognition between OFL and aptamer(OFL) could not only react with •O2 – but also produce chemiluminescence emission, resulting in the “signal-on” state. Because of the signal “on–off–on”, the proposed aptasensor exhibited wide linear ranges for CIP (0.001–100 ng/mL) and OFL (0.0005–100 ng/mL) detection. Furthermore, the low detection limits of CIP (0.06 pg/mL) and OFL (0.022 pg/mL) could achieve the ultrasensitive analysis.

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A highly sensitive and dual-readout immunoassay for norfloxacin in milk based on QDs-FM@ALP-SA and click chemistry

Cited by 16 publications

References 37 publications

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

Fluorescent strip sensor for rapid and ultrasensitive determination of fluoroquinolones in fish and milk

A Direct Z-Scheme AgBr/CuBi₂O₄ Photocathode for Ultrasensitive Detection of Ciprofloxacin and Ofloxacin by Controlling the Release of Luminol in Self-Powered Microfluidic Photoelectrochemical Aptasensors

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A highly sensitive and dual-readout immunoassay for norfloxacin in milk based on QDs-FM@ALP-SA and click chemistry

Cited by 16 publications

References 37 publications

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

Fluorescent strip sensor for rapid and ultrasensitive determination of fluoroquinolones in fish and milk

A Direct Z-Scheme AgBr/CuBi2O4 Photocathode for Ultrasensitive Detection of Ciprofloxacin and Ofloxacin by Controlling the Release of Luminol in Self-Powered Microfluidic Photoelectrochemical Aptasensors

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A Direct Z-Scheme AgBr/CuBi₂O₄ Photocathode for Ultrasensitive Detection of Ciprofloxacin and Ofloxacin by Controlling the Release of Luminol in Self-Powered Microfluidic Photoelectrochemical Aptasensors