A highly phagocytic cell line TO from Atlantic salmon is CD83 positive and M-CSFR negative, indicating a dendritic-like cell type

Pettersen, Eirin Fausa; Ingerslev, Hans-Christian; Stavang, Vibeke; Egenberg, Marie; Wergeland, Heidrun I.

doi:10.1016/j.fsi.2008.08.014

Cited by 44 publications

(34 citation statements)

References 59 publications

(87 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…6C, 6D). These results are consistent with reports that salmonid monocytes/macrophages are positive for BG and NCAE stains while neutrophils are positively stained with MPO and SBB stains (47, 48). The distribution of CD4-1 + myeloid cells was determined by flow cytometry and revealed that significant percentages of CD4-1 + myeloid cells were found in the myeloid cell population of blood (~14%) and HK (~14.9%), whereas relatively low percentages of these cells were observed in spleen (~6.4%) and thymus (~2.8%) (Fig.…”

Section: Resultssupporting

confidence: 93%

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

Takizawa

Magadán

Parra

et al. 2016

The Journal of Immunology

104

View full text Add to dashboard Cite

Tetrapods contain a single CD4 co-receptor with four immunoglobulin domains that likely arose from a primordial two-domain ancestor. Notably, teleost fish contain two CD4 genes. Like tetrapod CD4, CD4-1 of rainbow trout includes four immunoglobulin domains while CD4-2 contains only two. Since CD4-2 is reminiscent of the prototypic two-domain CD4 co-receptor, we hypothesized that by characterizing the cell types bearing CD4-1 and CD4-2, we would shed light into the evolution and primordial roles of CD4-bearing cells. Using newly established monoclonal antibodies against CD4-1 and CD4-2, we identified two bona fide CD4+ T-cell populations, a predominant lymphocyte population co-expressing surface CD4-1 and CD4-2 (CD4 DP), and a minor subset expressing only CD4-2 (CD4-2 SP). While both subsets produced equivalent levels of Th1, Th17, and Treg cytokines upon bacterial infection, CD4-2 SP lymphocytes were less proliferative and displayed a more restricted TCRβ repertoire. These data suggest that CD4-2 SP cells represent a functionally distinct population and may embody a vestigial CD4+ T cell subset, the roles of which reflect those of primeval CD4+ T cells. Importantly, we also describe the first CD4+ monocyte/macrophage population in a non-mammalian species. Of all myeloid subsets, we found the CD4+ population to be the most phagocytic, while CD4+ lymphocytes lacked this capacity. This study fills in an important gap in the knowledge of teleost CD4-bearing leukocytes thus revealing critical insights into the evolutionary origins and primordial roles of CD4+ lymphocytes and CD4+ monocyte/macrophages.

show abstract

Section: Resultssupporting

confidence: 93%

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

Takizawa

Magadán

Parra

et al. 2016

The Journal of Immunology

104

View full text Add to dashboard Cite

show abstract

“…Previously MH II transcripts have been detected in RTS11 and in the highly phagocytic cell line TO from Atlantic salmon [29]. For fibroblast cell lines, MH IIb transcripts have been shown to be absent in the catfish GF5 (10) but present the rainbow trout RTG-2 [27], as confirmed here.…”

Section: Mh Iibsupporting

confidence: 84%

A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA

Kawano

Kales

Fujiki

et al. 2010

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…In the present study, RGAs were also performed with TO cells, a cell line derived from salmon head kidney that possesses some characteristics of dendritic cells (46). Surprisingly, although the synergy between SsMyD88 and SsIRF3 was confirmed, SsMyD88 overexpression suppressed the superior IRF7A/B-induced activation of the Mx1 and the ISRE reporters in TO cells (Fig.…”

Section: Discussionmentioning

confidence: 57%

MyD88 Interacts with Interferon Regulatory Factor (IRF) 3 and IRF7 in Atlantic Salmon (Salmo salar)

Iliev

Sobhkhez

Fremmerlid

et al. 2011

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: MyD88 directly interacts and affects IRF activation in mammals, but no information has been available about lower vertebrates. Results: Transgenic MyD88 interacts with IRF3 and IRF7A/B and modulates the IRF-induced IFN response and accumulates in aggresomes in Atlantic salmon. Conclusion: MyD88 is involved in the regulation of the IRF-induced IFN response in Atlantic salmon. Significance: The results shed light on the evolution of the innate immune response in vertebrates.

show abstract

A highly phagocytic cell line TO from Atlantic salmon is CD83 positive and M-CSFR negative, indicating a dendritic-like cell type

Cited by 44 publications

References 59 publications

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA

MyD88 Interacts with Interferon Regulatory Factor (IRF) 3 and IRF7 in Atlantic Salmon (Salmo salar)

Contact Info

Product

Resources

About