A high-yield method for the extraction and purification of capsaicin

Sass, Neil L.; Rounsavill, Mark; Combs, Harold F.

doi:10.1021/jf60214a035

Cited by 12 publications

(4 citation statements)

References 3 publications

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“…A molecule of capsaicin contains a phenolic hydroxyl group and an amide bond. Capsaicin is a weak polar acidic compound soluble in alkaline aqueous solutions (Sass et al 1977). Inorganic salts should be distinguished from alkaline salts, such as sodium carbonate, sodium hydroxide, and potassium hydrogen phosphate.…”

Section: Resultsmentioning

confidence: 99%

Three-liquid-phase extraction and separation of capsanthin and capsaicin from Capsicum annum L.

Dang¹,

Zhang²,

Xiu³

2014

Czech J. Food Sci.

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The extraction of capsanthin and capsaicin from red pepper (Capsicum annum L.) was studied using a three-liquid-phase system (TLPS) of acetone/K2HPO4/n-hexane. When the system consisted of 22% (w/w) acetone/20% (w/w) K2HPO4/10% (w/w) n-hexane, capsanthin was extracted into the top n-hexane-rich phase, yielding a recovery of 98.15% at a temperature of 25°C. Meanwhile, capsaicin was mainly distributed in the middle acetone-rich phase, less than 0.01% in the top phase, and undetectable in the bottom salt-rich phase. The yields of capsanthin and capsaicin were 105 and 88% of those of the conventional solvent extraction, respectively. Thus, capsanthin and capsaicin were separated through a single step at a low cost.

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Section: Resultsmentioning

confidence: 99%

Three-liquid-phase extraction and separation of capsanthin and capsaicin from Capsicum annum L.

Dang¹,

Zhang²,

Xiu³

2014

Czech J. Food Sci.

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“…Capsaicin was isolated from red pepper of 'Kanthari' chilli according to the method developed by Neil L Sass et al 1977 with slight modification [40]. Briefly, 2 kg of fresh, riped chilli peppers were washed thoroughly with water.…”

Section: Isolation and Characterization Of Capsaicinmentioning

confidence: 99%

Capsaicin-loaded solid lipid nanoparticles: design, biodistribution, in silico modeling and in vitro cytotoxicity evaluation

Kunjiappan

Murugesan²,

Kumar³

et al. 2020

Nanotechnology

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Lower doses of capsaicin (8-methyl-N-vanillyl-6-nonenamide) have the potential to serve as an anticancer drug, however, due to its pungency, irritant effect, poor water solubility and high distribution volume often linked to various off-target effects, its therapeutic use is limited. This study aimed to determine the biodistribution and anticancer efficacy of capsaicin loaded solid lipid nanoparticles (SLNs) in human hepatocellular carcinoma in vitro. In this study, SLNs of stearic acid loaded with capsaicin was formulated by the solvent evaporation-emulsification technique and were instantly characterized for their encapsulation efficiency, morphology, loading capacity, stability, particle size, charge and in vitro drug release profile. Synthesized SLNs were predominantly spherical, 80 nm diameter particles that proved to be biocompatible with good stability in aqueous conditions. In vivo biodistribution studies of the formulated SLNs showed that 48 h after injection in the lateral tail vein, up to 15% of the cells in the liver, 1.04% of the cells in the spleen, 3.05% of the cells in the kidneys, 3.76% of the cells in the heart, 1.31% of the cells in the lungs and 0% of the cells in the brain of rats were determined. Molecular docking studies against the identified targets in HepG2 cells showed that the capsaicin is able to bind Abelson tyrosine-protein kinase, c-Src kinase, p38 MAP kinase and VEGF-receptor. Molecular dynamic simulation showed that capsaicin-VEGF receptor complex is highly stable at 50 nano seconds. The IC50 of capsaicin loaded SLNs in HepG2 cells in vitro was 21.36 μg × ml−1. These findings suggest that capsaicin loaded SLNs are stable in circulation for a period up to 3 d, providing a controlled release of loaded capsaicin and enhanced anticancer activity.

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“…However, the pepper oils are non-saponifiable lipids implying that they are non-saturated fatty acids and so can be persevered for some time without spoilage or peroxidation if there is a successful separation of water or an extraction done using acetone. 20,21 This way they may be useful for industrial purposes such as in the production of cosmetics, insecticides, flavoring, beverages, candies and drinks.…”

Section: Essential Oil Of Capsicum Peppersmentioning

confidence: 99%

Potential health benefits of conventional nutrients and phytochemicals of Capsicum peppers

R¹

2018

PPIJ

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Capsicum peppers are great herbal medicine bestowed with natural pharmacological bioactive compounds majorly Phytochemicals, capsaicin, essential oils and a rich mix of organic micronutrients with potential health benefits. They play nutritional roles in diets and possess medicinal properties. These bioactive agents manifest their usefulness over mono-therapy by working in consortium and exert synergistic, polyvalent and pharmacological effects in humans as nutraceutical that serve as functional foods; as crude drugs for health benefits including prevention and treatments of chronic metabolic diseases such as coronary heart disease, diabetes and obesity. The synergistic effects of the phyto-nutrients of Capsicum peppers are thought to make them potentially effective anti-microbial, anti-inflammatory, anticancerous, anti-tumor, anti-mutagenic and possess good antioxidants with scavenging ability for singlet oxygen, free radical inhibitors, peroxide decomposers and metal chelating agents which are implicated in favoring of the onset of degenerative diseases including mucosa degeneration. While capsaicin imparts the ability for Capsicum peppers to function as topical agents, for the relief of various forms of pains as pain medicine; its activity as neurotoxic and cytotoxic agent is suggested to give various potential environmental health benefits and also benefiting man as a potential effective anesthesia. The presence of Phytochemicals in Capsicum pepper makes them not only useful and much cheaper alternatives to synthetic drugs but the Phytochemicals can be harnessed as crude drugs for the production of novel drugs. However, clinical studies on the consumption of Capsicum peppers are insufficient and needed to confirm some of the findings from animal studies.

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A high-yield method for the extraction and purification of capsaicin

Cited by 12 publications

References 3 publications

Three-liquid-phase extraction and separation of capsanthin and capsaicin from Capsicum annum L.

Three-liquid-phase extraction and separation of capsanthin and capsaicin from Capsicum annum L.

Capsaicin-loaded solid lipid nanoparticles: design, biodistribution, in silico modeling and in vitro cytotoxicity evaluation

Potential health benefits of conventional nutrients and phytochemicals of Capsicum peppers

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