A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics

Jenkins, Christopher W.; Jenkins, Rhys; Pryse, Meleri M; Welsby, Kathryn A.; Jitsumura, Maki; Thornton, Catherine A.; Dunstan, P. R.; Harris, Dean

doi:10.1039/c8an01323c

Cited by 45 publications

(44 citation statements)

References 25 publications

(28 reference statements)

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“…[85] fitted a seventh-order polynomial function over the 400-1800 cm -1 region and subtracted from the raw serum spectrum to remove the unwanted background, providing an effective baseline correction, and spectra were normalised by the area under the curve prior to data analysis. Least [29]. The phenyl alanine peak was chosen for normalisation as it is the sharpest and most intense peak within the serum spectra and it is observed that normalisation to this peak produced better diagnostic discriminatory results when compared to other normalisation methods such as vector normalisation.…”

Section: Data Preprocessingmentioning

confidence: 99%

“…The adapted EMSC algorithm can also be employed to remove known spectral interferents. In several studies of human serum, β-carotene has been observed as a string contributor to the spectrum, particularly when using 532nm or lower as source, as the scattering from the conjugated antioxidant species are resonantly enhanced [29,50,83]. The LMWF species are not easily removed by centrifugal separation [69], but their contributions can be effectively "digitally" removed using the adapted EMSC protocol.…”

Section: S~ [ × ] + [ × ] + [∑ ]mentioning

confidence: 99%

“…Raman microspectroscopy potentially lends itself naturally to the analysis of liquid biofluids and such applications have attracted considerable attention in recent years. There have been, however, multiple strategies that are used to perform such real-time analysis and these options must be carefully considered to achieve optimum Raman spectra from liquid samples [28,29]. Several studies have reported the proof-of-concept of liquid sample analysis using Raman spectroscopy [30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…Jenkins et al developed a high-throughput (HT) serum Raman spectroscopy platform and compared dry and liquid data acquisition of serum samples for liquid biopsy of 30 colorectal cancer patients and 30 matched control patients [29]. Using a stainless steel high throughput substrate that allows up to 40 samples to be loaded at once, and 785 nm laser as the excitation wavelength, the maximum sensitivity and specificity obtained for discrimination of colorectal cancer patients were 77% and 81% respectively.…”

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confidence: 99%

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Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

2020

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There is compelling evidence in the literature to support the application of Raman spectroscopy for analysis of bodily fluids in their native liquid state. Naturally, the strategies described in the literature for Raman spectroscopic analysis of liquid samples have advantages and disadvantages. Herein, recent advances in the analysis of plasma/serum in the liquid state are reviewed. The potential advantages of Raman analysis in the liquid form over the commonly employed infrared absorption analysis in the dried droplet form are initially highlighted.Improvements in measurement protocols based on inverted microscopic geometries, clinically adaptable substrates, data preprocessing and analysis, and applications for routine monitoring of patient health as well as therapeutic administration are reviewed. These advances suggest that clinical translation of Raman spectroscopy for rapid biochemical analysis can be a reality.In the future, this method will prove to be highly beneficial to clinicians for rapid screening and monitoring of analytes and drugs in the biological fluids, and to the patients themselves, enabling early treatment, before the disease becomes symptomatic, allowing early recovery.

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Section: Data Preprocessingmentioning

confidence: 99%

Section: S~ [ × ] + [ × ] + [∑ ]mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

2020

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“…There have been few studies of vibrational spectroscopic techniques for the advancement of colorectal cancer diagnostics based on the analysis of blood serum [13,14].…”

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confidence: 99%

Novel spectroscopic biomarkers are applicable in non-invasive early detection and staging classification of colorectal cancer

Miškovičová¹,

Frýba²,

Petruželka³

et al. 2021

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awareness of physical examination or the limitations of an early diagnostic level. Despite the improving standard of modern medicine, mortality from CRC is still remarkably high and the prognosis remains poor in many cases because of disease detection at advanced clinical stages. Raman spectroscopy yields precise information, not only regarding the secondary structure of proteins but also regarding the discrimination between normal and malignant tissues. We investigated whether this method can be used for the diagnosis of CRC including initial stages. To acquire more detailed structural information, we tested a novel diagnostic approach based on a suitable combination of conventional methods of molecular spectroscopy (Raman and Fourier transform infrared) with advanced, highly structure-sensitive chiroptical techniques as electronic circular dichroism (ECD) and Raman optical activity (ROA) to monitor the CRC pathogenesis relating compositional, structural and conformational changes in blood biomolecules, some of which may be caused by pathological processes occurring during cancer growth, also at the beginning of the disease. Sixty-three blood plasma samples were analyzed using the combination of ECD and ROA supplemented by Raman and Fourier transform infrared (FT-IR) spectroscopies. The obtained spectra were evaluated together by linear discriminant analysis. The accuracy of sample discrimination reached 100% and the subsequent leave-one-out cross-validation resulted in 90% sensitivity and 75% specificity. There were also found the differences between the patients according to the clinical stage. The achieved results suggest a panel of promising biomarkers and indicate that chiroptical methods combined with conventional spectroscopies might be a new minimally invasive powerful tool for producing high-quality data, obtaining an accurate diagnosis of colorectal cancer through a peripheral blood sample, which is also able to determine the extent of this pathology. Further work needs to be carried out for these techniques to be implemented in the clinical setting.

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Serum Raman spectroscopy: Prognostic applications in oral cancers

et al. 2023

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Background Loco‐regional recurrences attributable to field cancerization and minimal residual cancer, remain prime causes of mortality in oral cancer (OC) subjects. The current study evaluates potential of serum Raman spectroscopy (SRS) to identify recurrence‐prone OC subjects. Methods Raman spectra of serum from eight healthy subjects (H) and 57 OC subjects (with‐recurrence [R], without‐recurrence [NR], and with suspicious‐lesions [S]), before (BS) and after (AS) surgical excision of tumor were recorded. OC subjects were followed‐up for 7‐years. Results DNA and protein alterations were observed in AS sera of all groups. 4‐, 3‐, and 2‐model multivariate analyses were used to stratify BS and AS groups. H spectra were 100% distinguishable from all other groups. AS, R and NR were distinguished with high accuracy (84%) in all models. No stratification (~50%) was observed BS. Conclusion SRS shows potential to identify recurrence prone subjects, post‐surgery, using serum collected as early as 1 week after surgery.

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A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics

Abstract: Translating Raman spectroscopy for colorectal cancer diagnosis with a focus on high-throughput design, inter-user variability and sample handling considerations.

Cited by 45 publications

References 25 publications

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

Novel spectroscopic biomarkers are applicable in non-invasive early detection and staging classification of colorectal cancer

Serum Raman spectroscopy: Prognostic applications in oral cancers

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