A High‐Throughput Assay of Cell‐Surface Interactions using Topographical and Chemical Gradients

Yang, Jing; Rose, Felicity R. A. J.; Gadegaard, Nikolaj; Alexander, Morgan R.

doi:10.1002/adma.200801942

Cited by 100 publications

(102 citation statements)

References 40 publications

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“…Another study developed substrates with microtopography gradients in one direction and with surface chemistry gradients in the orthogonal direction. [ 82 ] Fibroblast adhesion, proliferation and orientation were evaluated to create heat maps of cell behavior for the phase space of topography versus surface chemistry. These studies showcase polymer deposition to fabricate scaffolds containing a gradient in surface chemistry that induced uniform seeding of cells throughout porous scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial and High‐Throughput Screening of Biomaterials

2010

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Combinatorial and high-throughput methods have been increasingly used to accelerate research and development of new biomaterials. These methods involve creating miniaturized libraries that contain many specimens in one sample in the form of gradients or arrays, followed by automated data collection and analysis. This article reviews recent advances in utilizing combinatorial and high-throughput methods to better understand cell-material interactions, particularly highlighting our efforts at the NIST Polymers Division. Specifically, fabrication techniques to generate controlled surfaces (2D) and 3D cell environments (tissue engineering scaffolds) as well as methods to characterize and analyze material properties and cell-material interactions are described. In conclusion, additional opportunities for combinatorial methods for biomaterials research are noted, including streamlined sample fabrication and characterization, appropriate and automated bioassays, and data analysis.

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Section: Discussionmentioning

confidence: 99%

Combinatorial and High‐Throughput Screening of Biomaterials

2010

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“…[1][2][3] Considerable efforts are currently being focused on the understanding of the structural, mechanical, topographical and chemical cues cooperating, at different length scales, to dictate the biological response of a material and to determine the genotypic and phenotypic evolution of cells. [1] The goal is to translate this knowledge in fabrication technologies to reproduce and to exploit the observed complexity.…”

Section: Introductionmentioning

confidence: 99%

Direct Microfabrication of Topographical and Chemical Cues for the Guided Growth of Neural Cell Networks on Polyamidoamine Hydrogels

Reis

Fenili

Gianfelice

et al. 2010

Macromolecular Bioscience

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Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri-cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell-based arrays, stem cell therapy and cell-based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub-micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e-beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti-fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E-beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells.

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“…Two-dimensional gradients have also been reported, whereupon orthogonal to one gradient a second gradient is introduced. As such, every position on the gradient presents a unique combination of the two properties [27]. This gradient methodology is well suited to high throughput optimisation of a particular material property where a limited number of chemical components or properties are involved.…”

Section: Introductionmentioning

confidence: 99%

“…Gradient investigations employ the variation of the relative composition of two material properties by producing a sample that varies gradually, e.g. in chemical composition [26] or topography [27], across a convenient distance. Between the two compositional/morphological extremes, the response of the property of interest, e.g.…”

Section: Introductionmentioning

confidence: 99%

High throughput methods applied in biomaterial development and discovery

et al. 2010

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The high throughput discovery of new materials can be achieved by rapidly screening many different materials synthesised by a combinatorial approach to identify the optimal material that fulfils a particular biomedical application. Here we review the literature in this area and conclude that for polymers, this process is best achieved in a microarray format, which enable thousands of cell-material interactions to be monitored on a single chip. Polymer microarrays can be formed by printing pre-synthesised polymers or by printing monomers onto the chip where on-slide polymerisation is initiated.The surface properties of the material can be analysed and correlated to the biological performance using high throughput surface analysis, including time-of-flight secondary ion mass spectrometry (ToF-SIMS), Xray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements. This approach enables the surface properties responsible for the success of a material to be understood, which in turn provides the foundations of future material design. The high throughput discovery of materials using polymer microarrays has been explored for many cell-based applications including the isolation of specific 2 cells from heterogeneous populations, the attachment and differentiation of stem cells and the controlled transfection of cells.Further development of polymerisation techniques and high throughput biological assays amenable to the polymer microarray format will broaden the combinatorial space and biological phenomenon that polymer microarrays can explore, and increase their efficacy. This will, in turn, result in the discovery of optimised polymeric materials for many biomaterial applications.

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A High‐Throughput Assay of Cell‐Surface Interactions using Topographical and Chemical Gradients

Cited by 100 publications

References 40 publications

Combinatorial and High‐Throughput Screening of Biomaterials

Combinatorial and High‐Throughput Screening of Biomaterials

Direct Microfabrication of Topographical and Chemical Cues for the Guided Growth of Neural Cell Networks on Polyamidoamine Hydrogels

High throughput methods applied in biomaterial development and discovery

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